[Severe acute dental infections].
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Brain Abscess↗
Biomedical subjects
C Lindqvist
Publications and source records attributed to C Lindqvist.
Flow cytometric analysis of bioluminescence emitted by recombinant baculovirus-infected insect cells.
Five recombinant baculoviruses, each containing a different insect luciferase gene encoding a protein with characteristic light emission properties, namely, luc GR (546 nm), luc FF (556 nm), luc YG (560 nm), luc YE (578 nm), and luc OR (593 nm) were constructed. All genes were inserted under the transcriptional control of the polyhedrin gene promoter of the Autographa californica nuclear polyhedrosis virus (AcNPV) and expressed in Spodoptera frugiperda insect cells during viral infection. The biological activity of the different luciferases was characterized by using intact recombinant baculovirus infected cells. Addition of the substrate, D-luciferin, immediately prior to the analysis allowed monitoring of light emission by flow cytometry. Also, the kinetics of the light emission of lucGR was analyzed with the flow cytometer. The emission peaks of the infected cells were clearly separated by wavelength scanning. Especially, the firefly luciferase (lucFF) had a broad peak and transient luminescence. The highest maximal intensity values in vivo were recorded for luc GR and luc YG. SDS-PAGE analysis showed that the major protein expressed had a molecular weight similar to authentic luciferase. Flow cytometry and insect luciferases with clearly separated emission spectra appear to be of value for sensitive in vivo analysis of gene promoter activity.
Animals↗
Gingival and dental status, salivary acidogenic bacteria, and yeast counts of patients with active or inactive Crohn's disease.
To investigate dental, gingival, and salivary aspects during an exacerbation of Crohn's disease, 53 patients with long-standing Crohn's disease were clinically, endoscopically, and radiologically examined. The patients were divided in two groups: 32 patients had active disease and 21 patients had inactive disease. Oral examination of the patients did not reveal any statistically significant differences in gingival or dental indexes between the groups, but patients with active disease tended to have higher scores of gingivitis than patients with inactive disease despite the fact that the patients with active disease were younger than those in remission. No differences between the groups were observed in salivary flow rate or buffering capacity. Lactobacilli and yeast counts of saliva tended to be higher among patients with active disease than with inactive disease, but the difference between the groups was significant only for high mutans streptococci counts.
Adult↗
Analysis of possible factors leading to problems after nonsurgical treatment of condylar fractures.
PURPOSE: An attempt was made to identify types of condylar fractures that, despite active nonsurgical treatment, lead to unsatisfactory clinical results. PATIENTS AND METHODS: Ninety-two dentate adult patients with unilateral condylar fractures were studied clinically and radiologically. On the basis of the information collected, problematic cases were identified. These consisted of patients with persistent occlusal change and/or marked deviation of the mandible on mouth opening after removal of maxillomandibular fixation or during follow-up. Radiologic analyses were performed using panoramic and Towne's views, and the results of problematic and nonproblematic cases were compared. RESULTS: Using clinical parameters, two problem groups were found. In patients with persistent malocclusion, ramus height was significantly reduced, irrespective of degree or direction of angulation between the fragments. Deviation of the jaw on mouth opening, but no occlusal disturbances, occurred in those with dislocated condyles. CONCLUSION: Problematic condylar fractures can often be identified preoperatively by means of simple radiographic measurements and these cases should probably be treated surgically.
Adolescent↗
Sagittal ramus osteotomies fixed with biodegradable screws: a preliminary report.
Nine patients underwent bilateral sagittal split osteotomies of the mandible. The osteotomies were fixed using two self-reinforced poly-L-lactide screws. No maxillomandibular fixation was used postoperatively. The preliminary results with the shortest follow-up of 15 months show that primary healing was normal. No late-term clinical complications were encountered during the follow-up, the longest of which is now 23 months.
Adult↗
Effect of anticancer drugs on patients with and without initially reduced saliva flow.
We reported earlier that despite the well-known cytolytic effect of anticancer drugs, mean stimulated salivary flow rates were not significantly affected during a 12-month follow-up of patients undergoing treatment for lymphomas (Laine et al. Oral Oncol, Eur J Cancer 1992, 28B, 125-128). Therefore, we set out to investigate in more detail the flow rates and composition of salivas in these patients, but now grouped according to their initial flow rate values, which had been assessed before the start of treatment. 49 patients of the original material (30 men and 19 women, mean age 49.9 years) were divided into hyposalivation and normal flow rate groups, according to their baseline values. Stimulated saliva flow > or = 0.8 ml/min was regarded as the limit for a normal flow rate. 11 patients were found to have reduced flow at baseline (hyposalivation group), while 38 patients had normal flow rate. Analysis of repeated saliva samples taken during the 12-month follow-up showed that flow rates remained significantly lower in the hyposalivation group compared with those of the other group (P < 0.001). Further, the concentrations of total protein, albumin, lysozyme, amylase, IgG, IgA and IgM were, and remained, all significantly higher in patients of the hyposalivation group. Counts for salivary mutans streptococci and yeasts were higher and remained significantly so among patients with hyposalivation than among those with normal flow rate while lactobacilli counts were higher in patients with normal initial flow rate.(ABSTRACT TRUNCATED AT 250 WORDS)
Adult↗
Cancers of the lip and oropharynx in different social and occupational groups in Finland.
Social status and occupation-specific risks of cancers of the lip, tongue, mouth and pharynx were studied in a nationwide series of 2369 men and 809 women diagnosed in Finland in 1971-1985 and recorded in the files of the Finnish Cancer Registry. Codes for social status (four classes) and occupation (336 categories) were drawn from the files of the 1970 Population Census. The standardised incidence rates (SIR) were defined as the ratios of observed and expected numbers of cases, the expected ones being based on the sex, age, site and calendar period-specific incidence rates in the Finnish population. The SIR of lip cancer in the lowest social class was five times that of the highest one, thus indicating the effects of social class differences in smoking habits. The effect of outdoor work on the development of lip cancer was also strongly supported by this study. The social class-adjusted SIR for fisherman was 2.7 (95% C.I. 1.3-5.0), for forestry managers 2.2 (1.2-3.6), for timber workers 1.9 (1.2-2.9) and for farmers 1.8 (1.2-2.6). For cancers of the oral cavity and pharynx there was no clear correlation with social class. However, the SIR for these cancers among farmers was significantly reduced. The occupation-specific SIRs pointed more to alcohol than smoking aetiology. In the case of cancer of the tongue, no aetiological clues whatsoever could be found. The role of direct occupational factors in the aetiology of any of these cancers seemed to be minimal.
Female↗
The effect of osteotomy and osteosynthesis in the mandibular condyle. A radiologic and histologic study in sheep.
Eighteen oblique osteotomies in sheep mandibular condylar necks were fixed with self-reinforced poly-L-lactide (SR-PLLA) (n = 9) or stainless steel (n = 9) screws (core diameters 2.4, 2.7, 3.0, or 3.5 mm). No intermaxillary fixation (IMF) was applied. The follow-up periods were 6, 12, and 24 weeks, respectively, after which the sheep were killed, and the status of the condylar head was assessed radiographically and histologically. The results showed that the fixation had been adequate. Bony destruction, osteophytes, and flattening of the condylar head, however, were frequently observed in both groups, as compared with the unoperated contralateral condyle. Histologic studies revealed that the thickness of the condylar head cartilage had increased and that the boundaries of the different cell layers were irregular, as was the junction of cartilage and bone. The diameter of the screw did not seem to have any influence on the occurrence of either radiologic or histologic signs of condylar degeneration. These findings indicate that screw fixation of condylar fractures in the mandible without IMF might result in degenerative joint disease.
Animals↗
Blindness after maxillofacial blunt trauma. Evaluation of candidates for optic nerve decompression surgery.
Clinical and computerized tomography (CT) findings in 10 patients with post-traumatic blindness after maxillofacial blunt trauma were evaluated as potential candidates for optic nerve decompression surgery. The total number of blind eyes was 14. All patients suffered from midfacial fractures (isolated zygomatic fracture in 4 and Le Fort fracture in 6 cases) predominantly caused by road traffic accidents. CT scanning and clinical findings allowed location of damage to the optic nerve region in all patients. The most common CT finding was swelling of the optic nerve. Fracture in the optic canal wall was seen in only 3 cases. The clinical situations characteristic of this type of injury make diagnosis and early surgical intervention difficult. It was concluded that two of the 10 patients might have benefitted from optic nerve decompression.
Adolescent↗
Risk of new primary cancer in patients with oropharyngeal cancer.
The relative risk of subsequent cancers was evaluated for a total of 9,092 patients with lip and oropharyngeal cancer recorded between 1953 and 1989 in the nationwide Finnish Cancer Registry. The observed numbers of patients were compared with those expected on the basis of the incidence rates in the Finnish population. There were 1,130 patients (12%) with a new cancer. The standardised incidence ratio (SIR) of contracting a new primary cancer was 1.2 for lip cancer patients (95% CI 1.1-1.3) and 1.4 for patients with oropharyngeal cancer (95% CI 1.2-1.4). Among lip cancer patients, a statistically significant excess risk was found for subsequent cancers in the oropharyngeal area (SIR 1.9, 95% CI 1.1-3.1), larynx (SIR 2.0, 95% CI 1.2-2.9) and lung (SIR 1.4, 95% CI 1.3-1.6), i.e. for cancers with tobacco aetiology. Among patients with oropharyngeal cancer there was an excess of lip cancer (SIR, 3.5, 95% CI 1.5-6.9), lung cancer (SIR 1.8, 95% CI 1.3-2.3) and leukaemia (SIR 2.3, 95% CI 1.0-4.3). Radiotherapy for the first primary did not increase the risk of new cancer.
Adult↗
Immunoaffinity purification of baculovirus-expressed rubella virus E1 for diagnostic purposes.
Three monoclonal antibodies, termed 4E10, 1E11:10, and 2D9:1, were generated against rubella virus. Immunoblot analysis with purified authentic rubella virus or recombinant baculovirus-expressed rubella virus structural proteins E1, E2, and C demonstrated that they were directed against the E1 envelope glycoprotein of the rubella virus particle. By using the yeast Ty virus-like particle system, it was possible to map the binding site of 1E11:10 within amino acids 236 to 286 of the E1 protein and the binding sites of 2D9:1 and 4E10 outside this region. Immunoaffinity purification with these monoclonal antibodies made it evident that they are useful for obtaining large quantities of pure baculovirus-expressed rubella virus envelope protein E1. The diagnostic potential of this immunoaffinity-purified recombinant rubella virus E1 protein compared with that of authentic rubella virus is demonstrated.
Animals↗
[Untreated teeth--a health risk].
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Bacterial Infections↗
Functional analysis of the human alpha 2C-C4 adrenergic receptor in insect cells expressed by a luciferase-based baculovirus vector.
A click beetle luciferase-based baculovirus expression vector is described for functional analysis and high level expression of a human alpha 2-adrenergic receptor (alpha 2AR) in Sf9 insect cells. The resultant recombinant baculovirus construct, AcLucGR-alpha 2(C4), was isolated by utilizing the light emitting properties of luciferase and used for abundant expression of the alpha 2C-C4 receptor protein in this lepidopteran insect cell line. A maximal expression of alpha 2-receptors at a level of 1.370 pmol/mg protein was obtained at 48 h after infection as determined by ligand-binding experiments using the alpha 2-receptor antagonist, [3H]rauwolscine. The receptor agonists, noradrenaline and clonidine, displaced the [3H]rauwolscine binding with Ki values 12.3 +/- 1.54 microM and 1.23 +/- 0.11 microM, respectively. The recombinant receptors were functionally intact since the agonists inhibited forskolin-stimulated cAMP production. Here, however, the maximal inhibition was obtained at 36 h after the infection. The results presented here, suggest that the baculovirus expression vector system (BEVS) provides a simple method for abundant expression of functional alpha 2-receptor subtypes. In addition, co-expression of luciferase proved to be useful for screening and isolation of the recombinant baculovirus.
Adrenergic alpha-Antagonists↗
Enhanced IL-4-mediated D10.G4.1 proliferation with suboptimal concentrations of anti-IL-4 receptor monoclonal antibodies.
Suboptimal concentrations of the anti-IL-4 receptor mAb M1 or M2 gave, in combination with IL-4, an enhanced proliferative response to the IL-4-responsive cell line D10.G4.1, compared to IL-4 alone. Increasing amounts of the M1 antibody inhibited the IL-4-dependent proliferation in a normal fashion. The enhanced IL-4-induced proliferation was only inhibited with the anti-IL-4 mAb 11B11, whereas the anti-IL-2 receptor antibodies had no effect. Addition of M1 or M2 antibodies to the IL-2-dependent cell line CTLL, known to express small amounts of IL-4 receptors and thereby also slightly responsive to IL-4, gave no enhanced IL-4-induced proliferation. Instead these antibodies were found to inhibit both the IL-4 as well as the IL-2-mediated CTLL proliferation.
Animals↗
Long-term stability of two different mandibular bridging systems.
To evaluate the benefits of narrow-beam roentgenography and spiral tomography for examination and monitoring of screw and plate fixation to bone we studied 22 rigid plate bridgings of mandibular defects. The plate reconstructions were of long duration or considered permanent. In 13 patients, AO-THORP (AO: Arbeitsgemeinschaft für Osteosynthesefragen; THORP: Titanplasma-beschichteten Hohl- und Vollkern-Rekonstruktionsplatten-Systems) reconstructions were used and in nine classic AO stainless steel plate bridgings. With the AO-THORP system, good bone apposition to the screws was evident in 96% (73/76 screws) of the cases. Fixation using the classic AO screws, however, was inadequate in 30% (20/66 screws) of the cases. Plate loosening was noted in 56% (5/9 plates). Examination using narrow-beam roentgenography and spiral tomography provided detailed information about bone resorption around the screws and provided a reliable tool for deciding on a follow-up regimen. When complications occur, they can be detected and treated early, reducing patient morbidity. In our opinion, classic AO plate bridging should always be considered temporary.
Bone Plates↗
Oral findings in patients with active or inactive Crohn's disease.
The frequency and type of oral mucosal lesions, dental infections, and salivary constituents were evaluated in 53 patients with Crohn's disease, who were divided into inactive, mildly active, and severely active groups on the basis of clinical and endoscopic criteria. Buccal biopsies from nine patients with active disease showed morphologic changes that suggested Crohn's disease-related lesions. Panoramic radiographs revealed more infectious foci in the teeth of patients with active Crohn's disease than in patients with inactive disease. Salivary flow rate, buffering capacity, total protein, amylase, and IgA and IgG concentrations did not differ with respect to the activity of Crohn's disease. The observed mucosal inflammation in patients with active Crohn's disease, although high in frequency, was mild and did not need therapy, but the great number of dental infections in association with the activity of Crohn's disease should be taken into account in the treatment of these patients.
Adult↗
Third molars confusing the diagnosis of carcinoma.
The purpose of this study was to describe cases of oral cancer in which the initial presenting symptoms were considered to be due to third molars and in which the underlying carcinoma therefore escaped early diagnosis. Between 1986 and 1990 a total of five cases with squamous cell carcinoma associated with a third molar (impacted, partially or totally erupted, or recently extracted) were treated at our unit. Because of acute or chronic symptoms related to disturbed eruption of third molars, the detection of a simultaneous malignant change and referral were delayed. In conclusion, all third molars, especially impacted, partially erupted, or overerupted ones, should be examined critically, especially if the patient has symptoms.
Adult↗
Decortication in the treatment of diffuse sclerosing osteomyelitis of the mandible. Retrospective analysis of 41 cases between 1969 and 1990.
Thirty-four patients with mandibular diffuse sclerosing osteomyelitis who had been treated by means of 61 decortications were evaluated retrospectively. Eighteen patients (53%) were free from symptoms on an average of 5.4 years after surgery. Of these, 12 had improved after their first operation. In the other six patients, decortication was performed two to four times before healing was clinically observable. Symptoms recurred in 75% of the cases within 12 months after surgery. Neither sex, location, extent, and chronicity of the disease nor the precise surgical technique used seemed to affect the outcome. The patients who exhibited improvement, however, were significantly older and more often edentulous than the patients in whom the symptoms recurred. Possible causes of failure were an insufficiently radical surgical procedure and retention of devitalized teeth in the decorticated area.
Adolescent↗