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Biomedical subjects

C Legendre

Publications and source records attributed to C Legendre.

233 records · Page 13Linked to original sources
PubMed1981

Inhibition of membrane-bound hepatic 3 hydroxy-3 methyl glutaryl CoA reductase as the consequence of altered membrane fluidity.

The activity of hydroxy methyl glutaryl-CoA reductase in microsomes from rat and from human liver was inhibited in a non-competitive manner by fenofibric acid. High affinity of the microsomal preparation for the ligand allowed a one-step purification of the microsomal enzyme preparation, using a Sepharose gel coupled to the phenol analogue of fenofibric acid. Ther Arrhenius plots of partially purified hydroxy methyl glutaryl-CoA reductase in the microsomal fraction from rat liver showed that the break in the activation energy at 11 degrees C was abolished by the ligand. The results in the present study may be consistent with a modulation of membrane-bound HMG-CoA reductase activity.

Animals↗
PubMed1979

Amydopyrine pancytopenia: detection of leucocytotoxic antibodies by a 51Cr-release test.

Amydopyrine is known to cause agranulocytosis by an immunological mechanism [2]. However, pancytopenia due to amydopyrine is extremely rare and usually mild and transient [3]. This report describes a patient with severe pancytopenia presumably due to amydopyrine sensitivity. Granulocytotoxic and lymphocytotoxic antibodies were detected in the patient's serum using a 51Cr-release test.

Aminopyrine↗
PubMed1975

The use of directly frozen cells in LDA assay.

A method of direct freezing is described which requires a cryoprotective medium consisting of human AB serum and DMSO, an ultra-low temperature freezer and a liquid-nitrogen refrigerator. Fresh and directly frozen cells were compared in the LDA assay. Direct freezing is quite reliable for normal lymphocytes, PHA transformed lymphocytes or leukaemic blasts used as targets. However, a controlled freezing procedure may be necessary to preserve the effector cell activity.

Antilymphocyte Serum↗
PubMedDate not supplied

Renal transplantation.

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Cell Adhesion Molecules↗
PubMedDate not supplied

[Rapamycine and mTOR inhibitors: from bench to bedside].

Rapamycin is a macrocyclic lactone with antifungal and antibiotic properties isolated from Streptomyces hygroscopicus during the 70's. Studies of rapamycin properties in yeast led to the discovery of TOR (Target Of Rapamycin) and its mammalian analogue, mTOR. mTOR is a central regulator of cell growth and proliferation in response to environmental stimuli such as growth factors or nutrients. There are two proteins that have been shown to be regulated by mTOR in response to a broad range of mitogenic stimuli. The translation regulation induced by mTOR is mediated by the p70 S6 kinase activation and the 4E-BP1 inhibition. Both proteins participate in the regulation of translation process and growth in cells stimulated by either mitogens or hormones. Antiproliferative effects of rapamycin and analogues have been demonstrated on numerous cell types, explaining the development of these drugs in clinical practice: as immunosuppressive drugs in solid organ transplantation, in oncology for the treatment of various types of cancer, and for the prevention of restenosis after coronary angioplasty. Rapamycin is a potent immunosuppressive drug used in solid organ transplantation for the prevention of acute rejection. In oncology these antiproliferative effects are evaluated in several types of cancers. Rapamycin is now widely used for coating stents to reduce post-stenting restenosis phenomenon after coronary angioplasty. Finally, rapamycin is now evaluated in various diseases characterized by proliferative disorders.

Animals↗
PubMedDate not supplied

[Improvement of blood filtrability with a purified extract of black currant anthocyanosides in cynomologus monkeys on a fat diet].

A fat diet has been administered for 3 months to Cynomologus monkeys treated with blackcurrant anthocyanosides as compared with others receiving no treatment. Blood filtrability of animals given a fat diet alone was significantly lowered (20.8%; p less than 0.01) as compared with that of animals receiving a standard diet. Blood filtrability of animals receiving a fat diet plus blackcurrant anthocyanosides was not significantly different from that of animals given a standard diet. Emphasis is laid on a new approach to the action of this type of product on blood vessel contents as compared with the known action on the container.

Animals↗