Search PubMed⌕ Search

Biomedical subjects

C Lane

Publications and source records attributed to C Lane.

86 records · Page 5Linked to original sources
PubMed1978

Requirement for 7-methylguanosine in translation of globin mRNA in vivo.

The 7-methylguanosine (m7G) residue present in the m7G5' ppp5'X-"CAP" structure of rabbit globin mRNA was removed quantitatively by periodate oxidation followed by beta-elimination in the presence of cyclohexylamine. The RNA thus treated was intact and exhibited no signs of degradation as examined by polyacrylamide gel electrophoresis in formamide. Assay for protein synthesis using a wheat germ cell-free system showed that the globin mRNA lacking m7G had lost most of its messenger activity. Identical treatment, of satellite tobacco necrosis virus (STNV) RNA, which does not contain the 5'-terminal "CAP" structure, resulted in no loss of its mRNA activity. Since the importance of the m7G residue in eukaryotic mRNA has not yet been shown essential for translation in vivo, both untreated and treated globin mRNAs were injected into frog oocytes and their translation into globin was measured at intervals over a ninety-six hour period. Globin mRNA either treated with periodate alone or lacking in m7g altogether were both found to have lost more than 90% of their activity in vivo.

Animals↗
PubMed1977

Subcellular compartmentation of albumin and globin made in oocytes under the direction of injected messenger RNA.

The Xenopus oocyte can be used to study the nature and specificity of the translational and post-translational systems of a normal living cell. We describe experiments combining messenger RNA microinjection and subcellular fractionation. Total Xenopus liver RNA directs the synthesis of albumin and vitellogenin contained within membrane vesicles; similarly, guinea pig mammary gland mRNA codes for membrane-bound protease-resistant milk proteins. In contrast, iodinated albumin protein injected into oocytes remains in the supernatant fraction, as does globin made on mRNA. The information encoded in the albumin messenger is therefore sufficient to specify synthesis of a membrane-bound product; moreoever, this highly efficient coupled translation-processing system may be of use in the study of the transfer of newly made proteins across membranes. A significant proportion (up to 20%) of newly made oocyte proteins enter a light membrane fraction, and many remain there. We speculate that these vesicles represent part of an important storage system.

Albumins↗
PubMed1977

Modified semiautomated method for free fatty acids in serum.

Modifications have been made to the reagent system of the semiautomated method for the determination of plasma free fatty acids by Baird et al. (1967), and results are reported of investigations into parameters affecting sensitivity and analytical range.

Animals↗
PubMed1975

Surveillance of some infectious diseases among aircrew personnel in Southeast Asia.

A 2-year analysis of specimens among aircrew personnel in Southeast Asia is reported. Stool specimens were examined for intestinal parasites and enteric bacteria, blood smears for blood parasites, and sera tested for transaminases (SGOT) and antibodies to Entamoeba histolytica, Toxoplasma gondii, Chikungunya and Japanese encephalitis viruses, Rickettsia tsutsugamushi, Leptospira sp. and Pseudomonas pseudomallei. One to four specimens each were obtained from 537 adult males and 20 females. There were 56 subjects with intestinal parasites as follows: Giardia lamblia (3.8%), Endolimax nana (2.6%), Entamoeba histolytica (1.4%), Entamoeba hartmanni (0.9%), Entamoeba coli (0.7%), Trichiuris trichiura (1.7%), Ascaris lumbricoides (1.4%), hookworm (0.9%), and Clonorchis sinensis (1.2%). Two individuals had malaria, Plasmodium vivax (0.6%). Pathogenic enteric bacteria were isolated from seven stool specimens; Shigella groups B and D (0.9%), Salmonella paratyphi (0.3%), and Arizona group (0.9%). Significantly elevated SGOT levels were found in 0.9% and antibodies to Japanese encephalitis virus in 1.5%, to Rickettsia tsutsugamushi in 1.2%, to Pseudomonas pseudomallei in 0.3%, to Entamoeba histolytica in 0.9% and to Toxoplasma gondii in 10.1%. No antibodies were found to Chikungunya virus or Leptospira sp.

Aerospace Medicine↗
PubMedDate not supplied

Radiotherapy outpatient review: a nurse-led clinic.

The study reported here was a descriptive account of the activities which take place at a traditional doctor-held radiotherapy review clinic. Comparison is made with a nurse-led clinic. Outcome measures, including number of interventions, consultation time, waiting time and degree of involvement with other support services were evaluated. Nurse consultations lasted significantly longer than those of the doctor, although waiting time was reduced. A greater number of referrals and liaison with other support services occurred and resulted in increased continuity of care. The results suggest that specialist nursing staff, given appropriate medical support, provide more effective care by using a holistic model for reviewing patients undergoing outpatient radiotherapy.

Ambulatory Care↗
PubMedDate not supplied

Leg ulcer care: an audit of cost-effectiveness.

Our objective was to establish an audit mechanism to determine the cost and effectiveness of leg ulcer care across two Health Authorities. Following identification of all patients with active ulcers over a two-month period, leg ulcer treatment, costs and outcome were prospectively audited over three months. We found that leg ulcer care is currently expensive and appears to be largely ineffective, especially when compared to the community clinic approach.

Cost-Benefit Analysis↗
PubMedDate not supplied

Family planning needs of adolescents.

Nurse practitioners working with teenagers need to take into consideration their level of psychosocial development. Examples of how nurse practitioners can provide family planning care to teenage clients based on their development are given. Two services, one center for young adolescents and one for college-age adolescents, are described.

Adolescent↗
PubMedDate not supplied

Principles of continuous quality improvement applied to intravenous therapy.

Documentation of the application of the principles of continuous quality improvement (CQI) to the health care setting is crucial for understanding the transition from traditional management models to CQI models. A CQI project was designed and implemented by the IV Therapy Department at Lawrence Memorial Hospital to test the application of these principles to intravenous therapy and as a learning tool for the entire organization. Through a prototype inventory project, significant savings in cost and time were demonstrated using check sheets, flow diagrams, control charts, and other statistical tools, as well as using the Plan-Do-Check-Act cycle. As a result, a primary goal, increased time for direct patient care, was achieved. Eight hours per week in nursing time was saved, relationships between two work areas were improved, and $6,000 in personnel costs, storage space, and inventory were saved.

Decision Trees↗