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Biomedical subjects

C Lambert

Publications and source records attributed to C Lambert.

At least 199 records · Page 11Linked to original sources

Psychological complications from the use of hypno-sedatives in dental practice.

The use of hypno-sedatives in dentistry, diazepam, and nitrous oxide, may result in psychological sequelae for the patient. These problems are not remedied by technical approaches to treatment. They may be decreased by a better understanding of suggestion, personality, and anxiety. The study of the use of suggestion is recommended.

Abreaction↗

An evaluation of elastic tissue staining.

The Special Procedures Laboratory of the Department of Pathology at M.D. Anderson Hospital was concerned with the nationwide shortage of hematoxylin. The amount of this dye required for various staining solutions was calculated to determine thrifty usage. Some solutions required more hematoxylin than was available and prompted the serious investigation of existing techniques and new methods for routine laboratory use. Evaluation of various techniques resulted in a significantly successful modification of Gomori's aldehyde fuchsin technique, which was subsequently adopted as a routine procedure for elastic tissue staining in the Special Procedures Laboratory.

Aldehydes↗

Intranuclear localization of mercury in vivo.

Purified nuclei isolated from mice challenged with nonlethal levels of mercury chloride (10-(3)M) in drinking water for 4 to 7 weeks (experimental) and from animals given deionized water (control) were fractionated and the subsequent fractions were analyzed for mercury by flameless atomic absorption. Control (active) euchromatin contained 1.75 +/- 0.53 micrograms of mercury per milligram of DNA. There was a 12- to 15-fold enrichment of mercury in the euchromatin fraction of challenged animals. Mercury was not detected in control (inactive) heterochromatin, and only trace levels (parts per billion) appeared in experimental heterochromatin. It seems likely that mercury can be incorporated into chromatin as a metal-protein complex, but the possibility of protein-mercury-DNA or mercury-DNA complexes within euchromatin cannot be excluded.

Animals↗