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Biomedical subjects

C Lagarde

Publications and source records attributed to C Lagarde.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

Selective uptake and sustained expression of AAV vectors following subcutaneous delivery.

BACKGROUND: Recombinant adeno-associated viral (rAAV) vectors are capable of long-term expression of secreted and intracellular proteins following delivery to muscle, liver, and the central nervous system. In this study, we have evaluated subcutaneous injection of rAAV encoding a variety of transgenes as an alternative route of administration for the systemic delivery of therapeutic proteins. METHODS: rAAV vectors encoding the human factor IX, human interferon-alpha 2a, murine erythropoietin (epo), and Escherichia coli lacZ genes were used for subcutaneous delivery into mature immunocompetent mice. Expression of factor IX and interferon in mouse serum was measured by ELISA. Expression of Epo was monitored by an increase in hemotocrit and by RIA. The tissue tropism of AAV transduction was determined by histochemistry following administration of the lacZ vector. RESULTS: Long-term protein expression (at least one year) is demonstrated in the serum of immunocompetent mice following subcutaneous delivery of AAV vectors encoding the human factor IX and interferon genes. The murine epo gene delivered via this route resulted in levels of Epo that correlate with increased hematocrits of up to 90% for a duration of nine months. rAAV encoding the lacZ gene revealed that the panniculus carnosus, a skeletal muscle layer of the skin, was transduced upon subcutaneous administration. CONCLUSIONS: This study shows that long-term expression of secreted proteins can be achieved using rAAV vectors injected subcutaneously as a single administration. The observation that the panniculus carnosus is the primary tissue transduced by rAAV illustrates the high tropism of rAAV for skeletal muscle.

Animals↗
PubMedDate not supplied

Distinctive clinical features between lymphoblastic and immunoblastic non-Hodgkin's malignant lymphomas (Kiel's classification) in a series of 141 patients.

Out of a series of 427 NHML based on the Kiel classification and without previous treatment, 186 were of high grade malignancy and among them were 83 lymphoblastic (LB) and 58 immunoblastic (IB) lymphomas which were included in this study. The LB and IB, which represent the majority of high grade NHML, were compared regarding their clinical features and courses. Eight main criteria were significantly different between these two groups: age of patient, immunological history, mediastinal and pleural involvement, primary or secondary involvement of the bone marrow, secondary leukaemia, secondary meningeal involvement, and involvement of the facial region. Some of these differences (mediastinum and meningeal involvement) are related to subtypes within LB. The differences between these two groups suggest that different treatment programs may be warranted.

Adult↗
PubMedDate not supplied

[Significance of chemotherapy complementary to radiotherapy in stages I and II in Hodgkin's disease].

In a series of 118 cases of Hodgkin's disease in stage I and II successively treated by irradiation alone or by chemotherapy followed by irradiation or by chemotherapy + irradiation + chemotherapy, a retrospective analysis brings out the share of classical radiotherapy in the cures associated with chemotherapy, which in providing a remission rate of the order of 90 p. 100 renders superfluous the maintenance of a long course of chemotherapy and of negligible interest the performance of an exploratory laparotomy.

Adolescent↗
PubMedDate not supplied

[Value of computer tomography during investigation of sub-diaphragmatic adenopathies occurring during the course of lymphomas and pelvic cancers (author's transl)].

A series of 60 patients were examined by abdominopelvic computer tomography 48 hours after foot lymphography. The diagnosis was Hodgkins's disease in 10 patients, lymphomas other than Hodgkin's in 20 cases, and 30 patients with pelvic carcinomas. The value of lymphography in lymphomas other than Hodgkin's is re-emphasized, and a good correlation exists between the results obtained and those observed with computer tomography, which is particularly useful for studying the upper lumbo-aortic regions. The group of Hodgkin's disease patients particularly demonstrates the limits of pelvic lymph node investigations with computer tomography, but supplies confirmation of the quality of examination of the lumbo-aortic chains. The group of patients with pelvic carcinomas is more heterogenous, and computer tomography is if greater value, mainly in cases with blocked lymphatics. Computer tomography examinations do not replace lymphography in the study of sub-diaphragmatic lymph node chains but are a useful complement as they demonstrate excluded or poorly opacified adenopathies. When lymphography is contra-indicated, computer tomography can supply valuable information, but the differential diagnosis of inguino-iliac chain lesions is difficult. Computer tomography is a logical procedure during pretreatment investigations whenever there is a doubt concerning possible lesions in the lumbo-aortic chains. It is the most valid guide for establishing details of a course of radiotherapy.

Adult↗