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Biomedical subjects

C L Prosser

Publications and source records attributed to C L Prosser.

At least 55 records · Page 3Linked to original sources
PubMed1974

A study of pace-maker activity in intestinal smooth muscle.

1. Electrical activity of longitudinal muscle from cat intestine was recorded in the double sucrose gap.2. Approximately 20% of the preparations demonstrated slow, spontaneous fluctuations of membrane voltage, slow waves. This activity, although quite uniform in a given preparation, showed considerable inter-preparation variation with respect to amplitude, frequency and wave form.3. Application of steady hyperpolarizing current decreased slow-wave frequency and increased slow-wave amplitude while depolarizing currents increased frequency and decreased amplitude.4. Some preparations with no spontaneous slow-wave activity developed slow waves when the membrane was hyperpolarized into a given range which, depending on the preparation, varied in size from 10 to 40 mV. Step or ramp depolarization of the membrane from hyperpolarized levels triggered slow waves in some preparations.5. When the membrane potential of a slow-wave generating preparation was clamped at the resting potential, spontaneous inward-directed current transients were observed.6. No changes in membrane conductance were observed during the course of a slow wave.7. The slow-wave pattern was simulated for individual preparations by applying the membrane current measured under voltage clamp to the passive membrane resistance and capacitance measured independently under current clamp.8. In addition to the defined slow-wave activity, voltage-dependent oscillations in membrane potential were sometimes observed.9. Application of 10(-5)M ouabain irreversibly blocked slow waves and produced a membrane depolarization equal to or slightly greater than the slow wave crest. Repolarization of the membrane to the resting potential, or hyperpolarization, failed to restore slow-wave activity.10. Removal of external potassium produced a reversible sequence of events almost identical to those following ouabain application.11. Replacement of 50% of the external sodium chloride with sucrose produced no changes in slow-wave activity with respect to rates of rise or fall, maximum amplitude or frequency. Sucrose replacement of all external sodium chloride eliminated slow waves after 5 min; however, activity could be restored by a slight hyperpolarization. Longer exposures to the modified bath abolished activity.12. Following a conditioning exposure to potassium-free Krebs solution, readmission of potassium at normal concentration produced a mean hyperpolarization of 20.5 mV and in spontaneous preparations an arrest of activity.13. Pump current in sodium-loaded, non-spontaneously active preparations was measured by voltage clamp and was observed to be voltage-dependent.14. The results of this study indicate that an electrogenic pump is present in longitudinal muscle of cat duodenum, and that oscillations in the level of pump current produce slow waves.

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PubMed1973

Potentiation by endotoxin of responses associated with increases in calcium conductance.

Bacterial endotoxin at concentrations of 0.03 mug/ml enhanced the following responses, each of which reflects calcium influx: (1) contraction of spiral intestine retractor muscle of dogfish; (2) amplitude of postsynaptic potential of squid giant synapse as a function of presynaptic depolarization; (3) membrane responses of deep abdominal extensor muscle of crayfish, stimulated intracellularly; and (4) slow transient (calcium) current in trabeculae of frog atrium under voltage clamp. Corticosteroid antagonized the endotoxin effect on the smooth muscle, not on the frog atrial current. One mechanism of action of endotoxin may be to increase transmembrane calcium currents.

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