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Biomedical subjects

C Kurashima

Publications and source records attributed to C Kurashima.

40 records · Page 3Linked to original sources
PubMed1985

Focal lymphocytic infiltration in thyroids of elderly people. Histopathological and immunohistochemical studies.

Histopathological studies were made on the thyroid glands obtained from 169 autopsy cases of patients, ranging in age from 63 to 97 years, who had not had collagen diseases. Focal lymphocytic infiltration was found in 29 (17.2%) of 169 thyroid glands. Although the relationship between the incidence and age was unclear among elderly people, there was a tendency of a prevalence in females with respect to severity of focal lymphocytic infiltration. Immunohistochemically, a predominance of T cells (80-90%) was found in the thyroid lesions. The infiltrating T cells were composed of chiefly OKT 4+ or Leu 3a+ subset (60-80%) and lesser number of OKT 8+ or Leu 2a+ subset (20-40%). An increase in the percentage of OKT 8+ or Leu 2a+ cells, however, was observed in the tiny lesions infiltrating the interfollicular connective tissues. In aged patients over 63 years old, the average weight of thyroid glands without lymphocytic infiltration, obtained from female patients, was approximately 11 g, and the average weight of those with focal lymphocytic infiltration was approximately 17 g, whereas there was no significant difference in the weight of thyroid glands obtained from male patients with and without focal lymphocytic infiltration (14 g). The findings in the present study suggest that focal lymphocytic infiltration in thyroid glands is a focal sign of an immunological disorder which is based on autoimmunity associated with aging.

Aged↗
PubMed1985

Production of monoclonal antibody against amyloid fibril protein and its immunohistochemical application.

An autopsy case of multiple myeloma (IgA-lambda) with extensive amyloid arthropathy of the systemic joints was described. Heavy deposits of amyloid (amyloidoma) were observed in the articular cavities of the joints. Furthermore, numerous amyloid deposits were found in the walls of small blood vessels in the general organs. Incubation of the paraffin sections of amyloid with potassium permanganate produced little loss of Congo red affinity and apple-green birefringence under polarized light. A crude preparation of amyloid protein was isolated from a frozen intra-articular mass, which had been obtained at necropsy, and injected into the footpads of BALB/c mice with complete Freund's adjuvant. The immune spleen cells were fused with myeloma cells (P3 X 63-Ag8.653) under the presence of polyethylene glycol. Hybridoma cell lines, producing supernatants which reacted not only with amyloid substances but also with normal human tissues, were omitted from the subjects of recloning, and one hybridoma cell line (Am-1) producing a specific monoclonal antibody (MAb) against the immunized amyloid substance was finally obtained. Using the indirect immunoperoxidase method, the specificity of MAb Am-1 was confirmed in the cryostat sections as well as in the formalin-fixed paraffin sections of various organs of the case from which the crude amyloid protein was obtained and used for immunization. Amyloid deposits in 25 cases with amyloidosis or amyloid deposits were examined with MAb Am-1, and 2 cases showed positive reactivity with Am-1. These 2 cases presented primary amyloidosis and focal amyloid deposits in the oral cavity, respectively; Congo red staining of amyloid in these cases showed resistance to treatment with potassium permanganate, as observed in the amyloid of the original case used for immunization. Trypsin treatment of the sections resulted in a loss of positive reactivity with MAb Am-1 in all these cases. This result indicates that Am-1 recognizes a substance composed of protein molecules. Furthermore, the immunohistochemical distribution of Am-1 positive reaction was consistent with the histological distribution of substance which could be stained by Congo red and showed apple-green birefringence under polarized light. These results have suggested that Am-1 reacts with a portion of amyloid protein which is resistant to treatment with potassium permanganate followed by Congo red staining.

Aged↗
PubMedDate not supplied

Immunological alterations with aging-laying a stress on recent progress in Japan.

Age-related decline of immune functions mainly occurs in the T-cell-dependent immune system and this is precede by physiological thymic involution starting after puberty. Age-related alterations of T cells are seen in their number, subsets and qualities. In relation to the qualitative change of aging T cells, disturbance of intracellular signal transduction is responsible for the impairment of proliferation and cytokine production upon antigenic stimulation. Thymus, but not bone marrow, is responsible for aging of the T-cell-dependent immune system. The thymic capacity to induce T cells starts to decline early in life. Older thymus induces different subsets of T cells which are functionally less active T cells than those of young thymus. In association with a decline in immune functions to exogenous antigens, autoimmune phenomena increase with advance of age, which are observed as production of autoantibodies and auto-reactive T cells. The possible site for controlling thymic function is discussed.

Journal Article↗