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Biomedical subjects

C Jones

Publications and source records attributed to C Jones.

At least 667 records · Page 37Linked to original sources
PubMed1981

Thermography in the assessment of peripheral joint inflammation--a re-evaluation.

The reproducibility and sensitivity of quantitative infra-red thermography as a measure of peripheral joint inflammation was reassessed. Experiments were carried out in a temperature-controlled room at 20 degrees C. Initial stabilization experiments showed that in normal, medium sized, joints, there was an initial rapid cooling phase followed by a slower cooling phase which lasted longer than two hours. In the knees the differences between normal and active rheumatoid joints increased the longer patients remained in the room but for practical reasons a 30-minute stabilization period was subsequently chosen. In views of hands and fingers, rebound increases in skin temperature after entering the room, together with lesser differences between inflamed and non-inflamed joints, were found. The results suggested that the thermographic technique examined was adequate for detecting inflammatory changes in knee, ankles and elbows but unsatisfactory for quantification of inflammation in the small joints of the hands.

Adult↗
PubMed1981

An acute lymphoblastic leukemia which produces human T cell growth factor.

We report the production of T cell growth factor activity by a PHA stimulated acute lymphoblastic leukemia cell line designated HSB-2. Factor production was enhanced by the addition of phorbol myristate acetate to the cultures. The growth factor will enhance the proliferation of PHA stimulated thymocytes and sustain the proliferation of growth-factor dependent human T cell blasts. The characteristics of the cell line are described.

B-Lymphocytes↗
PubMed1980

Domestic management: first and foremost--home.

Domestic management of mental handicap hospitals presents a challenge to staff who often have to cope with outdated methods of cleaning, difficult areas to clean, and insufficient money to spend on new equipment. Catherine Jones, a domestic services manager, outlines some of the problems of management in this type of hospital.

Hospitals, Special↗
PubMed1980

The family meets the hospital. A laboratory forecast of the encounter.

Families, like persons, can be considered as unitary cases. Like persons, families can be classified before treatment begins in an effort to predict their responses and thus, to formulate the best treatment program. Although many classification schemes have been proposed in the past, evidence is presented on the first successful use to our knowledge of a family classification scheme to predict a family's response to a family-oriented treatment program. The classification scheme grouped families according to their typical style of adapting to new situations. A family's classification was based on its performance in a standard laboratory problem-solving situation in which its interaction patterns could be measured precisely and objectively. This approach to classification successfully predicted the family's engagement in a family-oriented, inpatient treatment program for adolescents.

Adolescent↗
PubMed1980

Isolation and localization of DNA segments from specific human chromosomes.

Recombinant DNA techniques have been combined with somatic cell genetic methods to identify, isolate, and amplify fragments of human DNA localized at specific regions of human chromosome 11 selected as a model system. A library of genomic DNA segments has been constructed, in lambda Charon 4A bacteriophage, from the DNA of a somatic cell hybrid carrying a portion of human chromosome 11 on a Chinese hamster ovary cell background. Using a nucleic acid hybridization technique that distinguishes human and Chinese hamster interspersed, repetitive DNA, we have been able to distinguish recombinant phages carrying DNA segments of human origin from recombinant phages carrying DNA segments of Chinese hamster origin. We have isolated 50 human DNA segments thus far and have characterized 5 in detail. For each DNA segment characterized, a subsegment that carries no repetitive human DNA sequences has been identified. These segments have been used as hybridization probes in experiments that localize the DNA fragment on the chromosome. In each case an unequivocal chromosomal localization has been obtained with reference to a panel of hybrid cell clones each of which carries a deletion of a portion of the short arm of chromosome 11. At least one DNA segment has been identified which maps to each of the four regions on the short arm defined by the panel of hybrid cell clones used. The approaches described here appear to be general. They can be extended to produce a fine structure map of human chromosome 11 and other human chromosomes. This approach promises implications for human genetics generally, for the human genetic diseases, and possibly for understanding of gene regulation in normal and abnormal differentiation.

Animals↗
PubMed1980

How one family perceives another: the relationship between social constructions and problem-solving competence.

This paper delineates a rarely studied but important family process: how a family perceives and understands the relationships in other families it knows. We hypothesize that these perceptions of other families are fundamental components of a family's shared construing of its social world. Families differ in how they perceive other families, specifically, and in their approach to construing or apprehending their social world, generally. We have hypothesized that these general differences also play a crucial role in shaping the style and competence of family problem-solving. A family's approach to any problem depends upon how it construes or interprets the social context of that problem. Using these general concepts of family life, we construct specific hypotheses linking family problem-solving and interfamily perception. We describe methods for measuring both family problem-solving and how a family perceives other families. Findings confirm our major hypothesis: A family that searches for underlying and subtle patterns in a problem-solving situation will develop a more differentiated and organized conception of other families.

Adolescent↗
PubMed1980

Hyperemesis gravidarum due to thyrotoxicosis.

A case of hyperemesis gravidarum at 9 weeks' gestation is described for which no cause could initially be found. Intravenous feeding was needed as a life preserving measure and following improvement on this regime the patient went into a thyrotoxic crisis which was successfully diagnosed and treated. The continuing pregnancy and its outcome is described. The need for practitioners to remember vomiting as a presenting sign of thyrotoxicosis is stressed.

Adult↗
PubMed1980

Chromosomal assignment of the gene for folylpolyglutamate synthetase to human chromosome 9.

An auxotrophic mutant, GAT-, derived from the Chinese hamster cell line CHO-K1 and exhibiting multiple growth requirements for glycine, adenine, and thymidine, has been shown to be deficient in one of the folate-dependent enzymes, folylpolyglutamate synthetase (FPGS). This mutant was fused with normal human lymphocytes and human lymphoblasts and 41 GAT+ primary hybrid clones were isolated in medium lacking glycine, adenine, and thymidine. In addition, 71 secondary clones were isolated after growth of four primary hybrid clones in enriched medium, which allows losing the complementing human chromosome without losing cell viability. Analysis of human isozyme markers in the 112 primary and secondary clones established a syntenic relationship between the GAT marker and AK1, an isozyme marker for human chromosome 9. Enzyme studies of the parental and hybrid cells not only showed restored enzyme activities of FPGS in the hybrids, but also demonstrated that several enzyme characteristics of FPGS in the hybrids are consistent with those of the human enzyme. Thus, it is concluded that the human gene coding for the enzyme FPGS which complements the auxotrophic mutant GAT- in the CHO-K1 cells can be assigned to human chromosome 9. This assignment provides an additional selective marker for mammalian cell genetic analysis in which human chromosome 9 can be selectively and stably retained in the cell hybrids.

Animals↗
PubMed1980

Evidence for a steroid receptor in rheumatoid synovial tissue cells.

One mechanism by which glucocorticoids could exert their anti-inflammatory action is via rapidly saturable, stereo-specific cytoplasmic protein receptors. This report is of an investigation into such a possibility in synovial cells. Synovium, obtained from knee joints of rheumatoid patients undergoing surgery, was incubated with clostridiopeptidase A and trypsin-EDTA to obtain cell suspensions. These, together with cells obtained from synovial fluid aspirated from patients with rheumatoid arthritis, were identified by electron microscopy. Duplicate samples of these cell suspensions were incubated with increasing concentrations of H3Dexamethasone (1 x 10(-10)M-1 x 10(-9)M) for 30 minutes at 37 degrees C. Analysis of the proportion of steroid bound to whole cells showed evidence for specific, rapidly saturable, receptors in the cells obtained from synovial tissue, but this was not found in synovial fluid cells. Electron micrographs showed that cells obtained from synovial tissue consisted of synovial fibroblast - and macrophage-types, lymphocytes, monocytes and macrophages. Polymorphonuclear leucocytes appeared to be absent. However, in synovial fluid cell type polymorphonuclear leucocytes were the predominant cell type. We concluded from this, that one or more of the cell types present in synovial tissue contain a specific steroid receptor, but that this is lacking in synovial fluid polymorphonuclear leucocytes.

Arthritis, Rheumatoid↗