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Biomedical subjects

C Johansson

Publications and source records attributed to C Johansson.

At least 91 records · Page 5Linked to original sources
PubMed1998

A histomorphometric evaluation of screw-shaped implants each prepared with two surface roughnesses.

Four different surface modifications were designed. Forty screw-shaped implants were divided into 4 groups, 10 screws in each. Every screw was prepared with 2 different surface topographies. The surface topography was measured with a confocal laser scanning profilometer and the surface roughness was characterized using 1 height, 1 spatial and 1 hybrid descriptive parameter. After 12 weeks in rabbit bone all screws were histomorphometrically evaluated. Blasted surfaces demonstrated more bone in contact to implant surface compared with turned surfaces. Most bone in close contact to implant surface was found for a surface blasted with 75 microns sized particles, numerically characterized with an average height deviation (Sa) of 1.4 microns, an average wavelength (Scx) of 11.6 microns and a developed surface area ratio (Sdr) of 1.5.

Aluminum Oxide↗
PubMed1998

Analysis of the 5-HT1A receptor involvement in passive avoidance in the rat.

1. The effects of the 5-HT2A/2C agonist DOB, the selective 5-HT1A agonist NDO 008 (3-dipropylamino-5-hydroxychroman), and the two enantiomers of the selective 5-HT1A agonist 8-OH-DPAT (R(+)-8-OH-DPAT and S(-)-8-OH-DPAT) were studied in a step-through passive avoidance (PA) test in the male rat. 2. The 5-HT1A agonists injected prior to training (conditioning) produced a dose-dependent impairment of PA retention when examined 24 h later. R(+)-8-OH-DPAT was four times more effective than S(-)-8-OH-DPAT to cause an impairment of PA retention. Both NDO 008 and the two enantiomers of 8-OH-DPAT induced the serotonin syndrome at the dose range that produced inhibition of the PA response, thus, indicating activation of postsynaptic 5-HT1A receptors. 3. Neither NDO 008 nor R(+)-8-OH-DPAT induced head-twitches, a behavioural response attributed to stimulation of postsynaptic 5-HT2A receptors. In contrast, DOB induced head-twitches at the 0.01 mg kg(-1) dose while a 200 times higher dose was required to produce a significant impairment of PA retention. 4. The impairment of PA retention induced by both NDO 008 and R(+)-8-OH-DPAT was fully blocked by the active S(+)- enantiomer of the selective 5-HT1A antagonist WAY 100135 and the mixed 5-HT1A/beta-adrenoceptor antagonist L(-)-alprenolol. In contrast, the mixed 5-HT2A/2C antagonists ketanserin and pirenperone were found to be ineffective. Moreover, the beta2-adrenoceptor antagonist ICI 118551, the beta-antagonist metoprolol as well as the mixed beta-adrenoceptor blocker D(+)-alprenolol all failed to modify the deficit of PA retention by NDO 008 and R(+)-8-OH-DPAT. None of the 5-HT1A or 5-HT2A/2C receptor antagonists tested or the beta-blockers altered PA retention by themselves. 5. A 3 day pretreatment procedure (200+100+100 mg kg(-1)) with the tryptophan hydroxylase inhibitor p-chlorophenylalanine (PCPA) did not alter PA retention and did not prevent the inhibitory action of the 5-HT1A agonists, indicating that their effects on PA do not depend on endogenous 5-HT. 6. The effects of NDO 008 on PA were also studied using a state-dependent learning paradigm. NDO 008 was found to produce a disruption of PA when given either prior to training or retention or both prior to training and retention but it failed to affect PA retention when given immediately after training. .7 These findings indicate that the deficit of passive avoidance retention induced by the 5-HT1A agonists is mainly a result of stimulation of postsynaptic 5-HT1A receptors but not 5-HT2A receptors. The 5-HT1A receptor stimulation appears to interfere with learning processes operating at both acquisition and retrieval.

DOM 2,5-Dimethoxy-4-Methylamphetamine↗
PubMed1998

Increase in nitric oxide formation after chronic voluntary exercise in spontaneously hypertensive rats.

The effect of chronic voluntary exercise on the plasma level of nitrate, a major stable metabolite of nitric oxide (NO) was studied in spontaneously hypertensive rats (SHR). Exercise consisted of spontaneous running in wheels for 3-35 days. Blood samples were collected after 3, 7, 14, 21 and 35 days of exercise and all samples were drawn after the running wheel had been locked during the preceding 12 h. The plasma nitrate level was significantly (P < 0.05) elevated in SHR after 35 days of exercise. Surprisingly after 7 days of exercise a significant (P < 0.001) decrease in the nitrate level in plasma was noted. Further research is needed to elucidate this biphasic change in nitrate seen in this study. The elevated level of plasma nitrate seen after 35 days of voluntary exercise was still present up to 36 h after termination of exercise. We conclude that exercise training in SHR elicits an enhanced formation of NO.

Animals↗
PubMed1998

Pityrosporum orbiculare-reactive T-cell lines in atopic dermatitis patients and healthy individuals.

The yeast Pityrosporum orbiculare is one of the factors that may contribute to atopic dermatitis (AD). In the present study we compared the T-cell response to P. orbiculare in 12 AD patients with specific immunoglobulin (Ig)E antibodies (Ab) in serum against P. orbiculare with that of six non-atopic healthy controls. Freshly isolated peripheral blood mononuclear cells (PBMC) were cultured for 3 days in the presence of P. orbiculare extract. The proliferative response as measured by [3H]-thymidine incorporation was significantly higher in the AD patients than in the healthy controls (P < 0.05). Furthermore, significantly higher levels of interleukin (IL)-5 (P < 0.05), as analyzed by ELISA, were produced by PBMC from the AD patients compared to the healthy controls. Pityrosporum orbiculare-reactive T-cell lines (TCL) established by P. orbiculare stimulation of PBMC for 11 days produced significantly higher levels of IL-4 and IL-5 after stimulation with anti-CD3 Ab and showed a higher IL-4/interferon (IFN)-gamma ratio (P < 0.05) in the AD patients compared to the healthy controls. The higher proliferative PBMC response to P. orbiculare and the Th2-like cytokine production by P. orbiculare-stimulated TCL from AD patients indicate that P. orbiculare may play a role in maintaining skin inflammation in AD.

Adolescent↗
PubMed1998

A volumetric study of guided bone regeneration around titanium implants in the New Zealand white rabbit.

Previous studies have shown the ability for bone to grow under occlusive membranes. This study was undertaken to determine the time required for bone to form in the space created under the membrane and to determine the amount of bone that may be grown under the membrane. Thirty-two New Zealand white rabbits were divided into three groups. A Brånemark implant, having a diameter of 3.75 mm with a length of 7 mm, was placed in each tibia and Gore-Tex membrane was draped over the implant on the experimental side and tethered to the wound margin. Sixteen rabbits were sacrificed at six weeks, eight at twelve weeks, and eight at eighteen weeks. At six weeks the available space under the membrane was filled to 68 per cent, at twelve weeks it was 45 per cent, and at eighteen weeks 54 per cent. A comparison of bone height measurements on test and control sides showed a significant difference (p = 0.0001) at the three time intervals. A comparison of grown bone volumes (test vs control) was also statistically significant (p = 0.0001). The ability to grow bone under an occlusive membrane was confirmed but the long-term survival rate and ability to support load needs to be investigated.

Analysis of Variance↗
PubMed1998

Evidence that decreased heart rate in thyroid hormone receptor-alpha1-deficient mice is an intrinsic defect.

Using a telemetry system with implantable transmitters, we recorded heart rate, electrocardiogram (ECG), body temperature, and locomotor activity continuously in awake, freely moving mice deficient in the thyroid hormone receptor-alpha1 (TRalpha1). We have previously reported that the TRalpha1-deficient mice have a 20% lower mean heart rate and a 0.5 degrees C lower body temperature compared with wild-type control animals. In this study we found that when 3,5, 3'-triiodothyronine (T3) was given once a day, there was a parallel increase in heart rate (occurring 1 day later in the TRalpha1-deficient mice than in controls) and body temperature. Analysis of single-lead ECG revealed a prolonged QRS and Q-Tend time in the TRalpha1-deficient mice, which was shortened after T3 treatment. Monophasic action potential durations, measured in hearts from anesthetized mice at 90% of repolarization, were significantly prolonged in TRalpha1-deficient mice. Air-jet stress and a single injection of an anticholinergic agent induced a parallel increase, and a beta-adrenergic receptor blocker induced a decrease in heart rate in both groups. There was no difference in beta-adrenergic receptor density. The results indicate that the TRalpha1-deficient mice have a specific defect in intrinsic heart rate regulation.

Action Potentials↗
PubMed1997

Structural and catalytic properties of the expressed and purified NAD(H)- and NADP(H)-binding domains of proton-pumping transhydrogenase from Escherichia coli.

Proton-pumping nicotinamide nucleotide transhydrogenase from Escherichia coli contains three domains: the hydrophilic domains I and III harbor the binding sites for NAD(H) and NADP(H), respectively, and domain II represents the membrane-spanning region. Proton translocation involves primarily domain II but possibly also domain III, which contains the essential betaAsp392 residue. In the present investigation, the major portions of domain I (EcTHSalpha1 and EcTHSalpha2) and domain III (EcTHSbeta1) were overexpressed in E. coli and purified therefrom. EcTHSbeta1 was purified mainly in its holoform containing approximately 95% NADP+ and 5% NADPH. When combined, EcTHSalpha1/EcTHSalpha2 and EcTHSbeta1 were catalytically active, indicating native-like structures. Due to the lack of structural information and its possible role in proton pumping, EcTHSbeta1 was primarily characterized. Substrate-binding characteristics and conformational changes were investigated by fluorescence and CD. Fluorescence arising from the single betaTrp415 of EcTHSbeta1 was quenched upon binding of NADPH by resonance energy transfer, an effect that provides an important tool for investigating substrate interactions with this domain and the determination of Kd values. The apparent relative binding affinity for NADPH was found to be about 50 times higher than that for NADP+. Circular dichroism was used to estimate secondary structure content and for conformational analysis of EcTHSbeta1 in the absence and presence of added substrates at various temperatures. Results show that domain III complexed with NADPH or NADP+ adopts different conformations. Isoelectric focusing and native gel electrophoresis experiments support this finding. It is proposed that these structural differences play a central role in a conformationally-driven proton pump mechanism of the intact enzyme.

Amino Acid Sequence↗
PubMed1997

enod40 induces dedifferentiation and division of root cortical cells in legumes.

Under nitrogen-limiting conditions Rhizobium meliloti can establish symbiosis with Medicago plants to form nitrogen-fixing root nodules. Nodule organogenesis starts with the dedifferentiation and division of root cortical cells. In these cells the early nodulin gene enod40, which encodes an unusually small peptide (12 or 13 amino acids), is induced from the beginning of this process. Herein we show that enod40 expression evokes root nodule initiation. (i) Nitrogen-deprived transgenic Medicago truncatula plants overexpressing enod40 exhibit extensive cortical cell division in their roots in the absence of Rhizobium. (ii) Bombardment of Medicago roots with an enod40-expressing DNA cassette induces dedifferentiation and division of cortical cells and the expression of another early nodulin gene, Msenod12A. Moreover, transient expression of either the enod40 region spanning the oligopeptide sequence or only the downstream region without this sequence induces these responses. Our results suggest that the cell-specific growth response elicited by enod40 is involved in the initiation of root nodule organogenesis.

Journal Article↗
PubMed1997

Internal rectal intussusception seldom develops into total rectal prolapse.

PURPOSE: This study was designed to analyze how often internal rectal intussusception develops into total rectal prolapse. METHODS: Repeated investigations with defecography were performed in 312 patients because of persisting symptoms. In 79 patients who had a rectal intussusception at the first defecography, results of the second defecography and the patients' records were studied. RESULTS: A total of 38 patients had not undergone any surgical treatment of rectal intussusception or rectal prolapse between the first and second defecographies. One of these patients had a rectal prolapse at the second defecography, and another developed a clinical prolapse after the second defecography. CONCLUSIONS: The present study demonstrates that the risk of developing a rectal prolapse in patients with rectal intussusception is small. This risk should, therefore, not be used as an indication for surgery.

Administration, Oral↗
PubMed1997

Nitric oxide synthase inhibition blocks phencyclidine-induced behavioural effects on prepulse inhibition and locomotor activity in the rat.

The ability of the nitric oxide synthase inhibitor, NG-nitro-L-arginine methyl ester (L-NAME), to block the behavioural effects of the potent psychotomimetic, phencyclidine, was tested in rats using two different behavioural models. L-NAME was found to block both phencyclidine-induced disruption of prepulse inhibition of acoustic startle and phencyclidine-induced stimulation of locomotor activity. A selective action of L-NAME on the effects of phencyclidine was indicated, since L-NAME did not alter the effects of amphetamine, another potent psychotomimetic, in these behavioural models. These observations suggest that a nitric oxide-dependent mechanism may be involved in the effects of phencyclidine in the central nervous system.

Animals↗
PubMed1997

(-)Alprenolol potentiates the disrupting effects of dizocilpine on sensorimotor function in the rat.

The beta-adrenoceptor antagonist as well as serotonin 5-HT1 receptor antagonist, (-)alprenolol, was found to potentiate the disrupting effect of the noncompetitive NMDA receptor antagonist, dizocilpine, on prepulse inhibition (PPI) of the acoustic startle response (ASR) in the rat. The facilitating effect of dizocilpine on ASR amplitude was also potentiated by (-)alprenolol. (-)Alprenolol by itself did not affect either of these measures. These effects did not seem to be related to the unselective beta-adrenoceptor antagonist property of (-)alprenolol, since combined pretreatment with the beta1- and beta2-adrenoceptor antagonists, metoprolol and ICI 118551, did not alter the effects of dizocilpine on startle behaviour. However, a serotonergic influence was suggested by the fact that a facilitating effect of dizocilpine on ASR amplitude was also obtained by pretreatment with the 5-HT precursor, L-5-HTP, in benserazide-pretreated rats. Furthermore, pretreatment with the 5-HT2 selective receptor antagonist, MDL 100907, significantly reduced the (-)alprenolol-induced potentiation of the effects of dizocilpine on startle behaviour, while the 5-HT3 selective receptor antagonist, ondansetron, failed to do that. Finally, the (-)alprenolol-induced potentiation of the effects of dizocilpine was significantly reduced by pretreatment with the atypical antipsychotic, clozapine, and by the potential antipsychotic and selective dopamine D2 receptor antagonist, raclopride. This study suggests that altered 5-HT activity may influence the effects of psychotomimetic drugs such as dizocilpine on sensorimotor function, and this observation may have implications for the pharmacological treatment of schizophrenia in humans.

Alprenolol↗
PubMed1997

Crystal size and properties of superparamagnetic iron oxide (SPIO) particles.

The properties of a superparamagnetic iron oxide (SPIO) model contrast agent have been studied. The test material, HEP-SPIO, contained iron oxide multicrystal agglomerates coated with heparin, polyanionic, naturally occurring glycosaminoglycan. Fractionation of the HEP-SPIO suspension showed the existence of colloidally stable particles ranging from approx. 100 nm down to single crystal sizes. The small (< 20 nm) particles represented the major number fraction of particles present, but only approx. 2% of the total iron oxide mass. The volume weighted average diameter of the individual iron oxide crystals forming the multicrystal agglomerates was found to be 11-12 nm using transmission electron microscopy and vibrating sample magnetometry (VSM) techniques. Comparable results were obtained with X-ray diffraction and Mössbauer spectroscopy. A number of additional SPIO properties could also be determined on a routine VSM, such as the distribution standard deviation for the log-normal distribution of crystal sizes, the magnetic susceptibility, the magnetic remanence, and the intrinsic magnetization (magnetic moment) of the iron oxide. These parameters are useful tools for evaluation of the magnetic characteristics and contrast efficacy of SPIO contrast agents.

Chemical Fractionation↗
PubMed1997

Influence of implant diameters on the integration of screw implants. An experimental study in rabbits.

The influence of diameter on the integration of titanium screw-shaped implants was studied in the rabbit tibia by means of removal torque measurements and histomorphometry. Implants 3.0, 3.75, 5.0, and 6.0 mm in diameter and 6.0 mm long were inserted through one cortical layer in the tibial metaphyses of nine rabbits and allowed to heal for 12 weeks. The implants were then unscrewed with a torque gauge, and the peak torque required to shear off the implants was recorded. The histologic analysis in undemineralized ground sections comprised (1) a gross description of the implant sites and assessments of (2) the total implant length in bone and (3) in the cortical passage, as well as (4) the thickness of the cortical bone adjacent to the implants. From the removal torque values obtained and morphometric measurements, a mean shear stress value was calculated for each implant type. The biomechanical tests showed a statistically significant increase of removal torque with increasing implant diameter. The resistance to shear seemed to be determined by the implant surface in supportive cortical bone, whereas the newly formed bone at the periosteal and endosteal surfaces did not seem to have any supportive properties after 12 weeks. It is suggested that wide diameter implants may be used clinically to increase implant stability.

Animals↗
PubMed1997

Surface characterization and biological evaluation of spark-eroded surfaces.

Forty commercially pure titanium implants were prepared with a spark-eroding process in order to create highly increased surface roughnesses. Two degrees of roughness were achieved by altering the applied current. Surface topographical characterization was performed with SEM and an optical profilometer. The surface composition and oxide layer were investigated using Auger scanning microscopy. In the present study, there was a large difference between the stipulated and measured surface roughness, indicating the need for a careful surface characterization in each new study. After 12 wk in rabbit bone, no statistically significant difference was found with respect to peak removal torque and histomorphometric analyses. The results from the present study provide no support for further increase of the surface roughness than that possible to achieve with a blasting technique.

Journal Article↗
PubMed1997

The effects of triiodothyronine (T3) on heart rate, temperature and ECG measured with telemetry in freely moving mice.

We have set up a telemetry system and recorded heart rate, electrocardiogram (ECG), body temperature and the locomotor activity in awake freely moving mice. The telemetry system (DATA Sciences, St Paul, MN, USA) comprises a transmitter implanted in the peritoneal cavity and a receiver (RA1010) placed underneath the home cage. The signal from the transmitter includes the electrical activity of the heart and the body temperature. The results show that four days after the surgical procedure the mice have recovered and regained a clear circadian rhythm. The heart rate varied under baseline conditions between 432 and 618 beats min-1 and the body temperature between 35.1 and 37.7 degrees C (based on 60 min mean values). A clear time correlation between heart rate, body temperature and locomotor activity was found. As an evaluation of the method we injected T3 s.c. during a period of 4 days. Further, we were interested in whether it was possible to measure an integrated physiological response to T3 and further investigate the time course for the effect. After one day of treatment with triiodothyronine there was a significant increase in body temperature and locomotor activity. The increase in heart rate was seen after 2 days. The ECG recording revealed a significantly shortened QTend- and QRS-time. No significant difference in the PQ-time was found. This method may be of great importance in studies of genetically manipulated mice.

Animals↗
PubMed1997

Duration and mechanisms of the increased natural cytotoxicity seen after chronic voluntary exercise in rats.

We have recently shown that in vivo natural cytotoxicity is enhanced after chronic exercise in spontaneously hypertensive rats (SHRs). In the present report, we have studied the duration of this augmentation and some possible mechanisms involved. Exercise consisted of voluntary running for 4-5 weeks, with the running distance ranging from 2.7-15.6 km day(-1) during the last week of running. In vivo cytotoxicity was measured as clearance of injected 51Cr-labelled YAC-1 lymphoma cells from the lungs. The in vivo natural cytotoxicity was increased in running SHRs, and also in SHRs that had their running wheel locked for 24 and 48 h prior to the experiment, and was still present after 96 h. The enhancement of in vivo cytotoxicity after 5 weeks of exercise was abolished after an acute injection of the beta-adrenergic receptor antagonist timolol (0.5 mg kg(-1) i.v.), indicating that catecholamines are involved in this augmentation. Interestingly, 24 h after the last exercise bout, the increased natural cytotoxicity could be blocked by timolol. The opioid receptor antagonist naloxone given subcutaneously for 7 days by osmotic pumps (6 mg kg(-1) h(-1)) could not reverse the increased in vivo cytotoxicity seen in the running SHRs, suggesting that opioid receptor mechanisms are not involved, or at least not the naloxone-sensitive mu-receptor. Natural immunity was not influenced by the histamine H2 receptor antagonist ranitidine, either in controls or in runners, indicating that the natural killer cell-regulatory effect of histamine is not present in SHRs and does not seem to be involved in the exercise-induced changes in natural immune function. We conclude that the augmentation of in vivo natural cytotoxicity after voluntary chronic exercise in rats is long-lasting and that the augmentation is partly mediated by beta-adrenergic receptors.

Adrenergic beta-Antagonists↗
PubMed1996

Peptidyl conjugates of adenosine 5'-carboxylic acid synthesized and evaluated as ligands for P2 purinoceptors.

The role of extracellular ATP in vivo and the various cellular responses mediated by P2 purinoceptors have not yet been fully elucidated, in part depending on the lack of subtype-specific high affinity antagonists. Here we describe the synthesis of a new class of compounds, peptidyl derivatives of adenosine 5'-carboxylic acid, among which some have inhibitory effects in certain P2 purinoceptor-carrying biological systems, e.g., glioma and smooth muscle cell lines and isolated smooth muscle tissue preparations from guinea pig vas deferens and urinary bladder.

Adenosine↗