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Biomedical subjects

C Jia

Publications and source records attributed to C Jia.

At least 37 records · Page 2Linked to original sources
PubMed2000

[The effect of hyperoxic solution on acute myocardial hypoxia in scalded rabbits].

OBJECTIVE: To investigate the effects of hyperoxic solution on acute myocardial hypoxia in scalded rabbits. METHODS: Rabbits inflicted with 30% TBSA III degree scald injury and/or acute myocardial ischemia were employed as the model. Animals were randomly divided into 4 groups, i.e. scalded rabbits treated with hyperoxic solution (A), scalded rabbits treated with normal saline (B), scalded rabbits without any treatment (C(b)) and normal healthy rabbits (C(h)). The cardiac function, blood rheology, blood gas analysis and the ratio of infarction to ischemic areas were dynamically monitored. RESULTS: (1) The cardiac function of the rabbits in B group was evidently worse than that in C(h) group (P < 0.01) and A group (P < 0.05). (2) The whole blood viscosity in A, C(b) and especially B groups was obviously increased compared with that in C(h) group (P < 0.05). While the viscosity in A group was much lower than that in B group (P < 0.01), it was similar to that in C(b) group (P > 0.05). (3) The PO(2) in A group was significantly higher than that in B and C (C(b), C(h)) groups (P < 0.01). (4) The ratio of infarction to ischemic areas in A group was remarkably lower than that in B group (P < 0.01). CONCLUSION: The hyperoxic solution may exert beneficial effects on acute myocardial ischemia in scalded rabbits. The mechanism might be related to the immediate correction of hypoxia in vital organs with simultaneous lowering of blood viscosity and the establishment of myocardial collateral circulation after the infusion of hyperoxic solution.

Animals↗
PubMed2000

[The influence of hTGFbeta(1) gene transfection on the proliferation of NIH 3T3 fibroblast].

OBJECTIVE: To investigate the influence of hTGFbeta(1) ene transfection on the proliferation of in vitro cultured NIH 3T3 fibroblast. METHODS: Lipofictin mediated co-transfected method was employed to transfect hTGFbeta(1) plasmid DNA into NIH 3T3 fibroblasts of the rats. Cell growth counting method, MTT, flow cytometry (FCM) and soft agar clone forming method were adopted to examine the biology of the transfected cells. RESULTS: (1) Fibroblast growth slowed down after hTGFbeta(1) transfection, especially on 4th to 6th days after the transfection, which was accompanied by decreased synthesis of DNA as indicated by the increase of G(1) phase percentage (from 39.9% to 66.2% P < 0.05) and the decrease of S phase percentage (from 40.2% to 26.8%, P < 0.05); (2) There was no evident change of all the phases in a cell cycle in the blank load transfection group and in control group. (3) There was a good correlation between MTT and cell counting method (co-efficient 0.992) (4) The formation rate of the soft agar clone of fibroblast decreased from 1.18% to 0.55% (P < 0.05) after hTGFbeta(1) transfection. CONCLUSION: hTGFbeta(1) gene transfection could inhibit fibroblast proliferation, which might be related to the interference of TGFbeta(1) with the DNA synthesis of fibroblasts.

3T3 Cells↗
PubMed2000

[Simultaneous spectrophotometric determination of trace ferrum and cobalt with 5-Br-PADAP and Tween-80].

A new simultaneous method for the determination of ferrum and cobalt with 5-Br-PADAP by spectrophotometry in the presence of Twen-80 is studied. The results showed that in pH = 3.4 HAc-NaAc buffer medium, ferrum and cobalt can formed colored complexes with 5-Br-PADAP. Its maximum absorption wavelengths are at 585 nm. Beer's law is obeyed for the concentration of ferrum in the range of 0-10 micrograms.25 mL-1 and 0-12 micrograms.25 mL-1 of cobalt. The apparent molar absorptivities are epsilon 585Fe = 7.18 x 10(4) and epsilon 585Co = 9.27 x 10(4) respectively. This method has been applied to the determination of ferrum and cobalt in water sample with satisfactory results.

Azo Compounds↗
PubMed1999

Synaptic organization and neurotransmitters in the rat accessory olfactory bulb.

The accessory olfactory bulb (AOB) is the first relay station in the vomeronasal system and may play a critical role in processing pheromone signals. The AOB shows similar but less distinct lamination compared with the main olfactory bulb (MOB). In this study, synaptic organization of the AOB was analyzed in slice preparations from adult rats by using both field potential and patch-clamp recordings. Stimulation of the vomeronasal nerve (VN) evoked field potentials that showed characteristic patterns in different layers of the AOB. Current source density (CSD) analysis of the field potentials revealed spatiotemporally separated loci of inward current (sinks) that represented sequential activation of different neuronal components: VN activity (period I), synaptic excitation of mitral cell apical dendrites (period II), and activation of granule cells by mitral cell basal dendrites (period III). Stimulation of the lateral olfactory tract also evoked field potentials in the AOB, which indicated antidromic activation of the mitral cells (period I and II) followed by activation of granule cells (period III). Whole cell patch recordings from mitral and granule cells of the AOB supported that mitral cells are excited by VN terminals and subsequently activate granule cells through dendrodendritic synapses. Both CSD analysis and patch recordings provided evidence that glutamate is the neurotransmitter at the vomeronasal receptor neuron; mitral cell synapses and both NMDA and non-NMDA receptors are involved. We also demonstrated electrophysiologically that reciprocal interaction between mitral and granule cells in the AOB is through the dendrodendritic reciprocal synapses. The neurotransmitter at the mitral-to-granule synapses is glutamate and at the granule-to-mitral synapse is gamma-aminobutyric acid. The synaptic interactions among receptor cell terminals, mitral cells, and granule cells in the AOB are therefore similar to those in the MOB, suggesting that processing of chemosensory information in the AOB shares similarities with that in the MOB.

Animals↗
PubMed1999

[Experience in the management of severe inhalation injury].

OBJECTIVE: To summarize our clinical experiences in the treatment of severe inhalation injury accompanying extensive burn. METHODS: Retrospective analysis of 30 cases of inhalation injury (1980-1996) was done, the cure rate, mortality, effective treatments and lessons of failure were summarized. RESULTS: As soon as the diagnosis was defined, tracheostomy should be done immediately, followed by oxygen therapy, tracheo-bronchial lavage, aspiration, and clearance of airway secretion. If there was pulmonary edema or severe bronchospasm, cortical hormone was used for a short time, in addition, the patients were encouraged to cough, breathe deeply, and change postures, as well as patted on the back and also positioned to facilitate posture drainage, etc. These measures were effective. In this series, there were thirty cases of severe inhalation injury. Fourteen patients(46.6%) were cured, sixteen patients died(53.3%). The result was basically satisfactory. CONCLUSION: It is possible to increase the cure rate of severe inhalation injury, and the treatment should be carried out conscientiously as early as possible, and great attention should be paid to prevent various complications.

Adolescent↗
PubMed1999

[Effects of PCMV4-hTGF beta 1 as nucleic acid vaccine on II* burn wound healing and postburn scarring in rats].

OBJECTIVE: To investigate the gene expression of PCMV4-hTGF beta 1 as nucleic acid vaccine regulation and the effects on burn wound healing and scarring in rats and study the feasibility of gene therapy in burns. METHODS: Sixty wistar rats were divided into three groups: 1. burn + nucleic acid vaccine; 2. nucleic acid vaccine only(control); 3. burn only(control). ELISA was used to determine the dynamic changes in anti-TGF beta 1 neutralizing antibody in serum; determination of the quantities of DNA retained in muscles by Southern blot and in situ hybridization; observation of the ratio in collagen I/III during the wound healing by special stain method. RESULTS: The level of anti-TGF beta 1 antibody in serum reached the peak at the third week after naked DNA was injected, and a slight drop in the 4th week. TGF beta 1 plasmid DNA could be detected 5 minute after injection, and lasted 3 hours. In situ hybridization showed a positive staining in muscle fibers 5 days after injection. During the day 0-day 9, the wound healing speed in vaccine group was faster than control, and after day 10, no significant difference was found between groups, but the ratio of collagen I/III was reduced remarkably in vaccine group. CONCLUSION: Injection of PCMV4-hTGF beta 1 in rats can really cause general immune response reaction. It was showed that PCMV4-hTGF beta 1 was similar to TGF beta 1, and it had the effect of stimulating of epidermic cells to accelerate wound healing at early stage, and at later stage, it was similar to anti-TGF beta 1 neutralizing antibody, having the effect of inhibiting hyperplasia of scar. So it is confirmed that PCMV4-hTGF beta 1 as nucleic acid vaccine has the effect of promoting healing of burn wound and controlling the formation of scar.

Animals↗
PubMed1998

Neurogenesis and migration of receptor neurons in the vomeronasal sensory epithelium in the opossum, Monodelphis domestica.

The sensory epithelium of the vomeronasal organ (VNO) contains primary chemosensory receptor neurons that project to the accessory olfactory bulb (AOB). In the present study, neurogenesis and cell migration in the sensory epithelium of the VNO were analyzed in opossums (Monodelphis domestica) by using bromodeoxyuridine (BrdU) labeling. 1) In the VNO of normal adult opossums, BrdU labeled a small number of cells localized in the basal region of the sensory epithelium. After 1 or 2 weeks of survival, the labeled cells appeared in the receptor cell layers and became receptor neurons, as indicated by coexpression of the G proteins G(i alpha2) or G(o alpha). 2) In the VNO in which the receptor neurons had been destroyed by removing the AOB, the number of BrdU-labeled cells in the reconstituting sensory epithelium was greatly increased compared with that in the intact VNO. The labeled cells were also located in the basal region of the sensory epithelium. 3) In the developing VNO (at postnatal day 10), more cells in the basal region of the sensory epithelium were labeled than in the adult VNO, indicating rapid cell proliferation; and there appeared to be more labeled cells in the basal region near the margins of the sensory epithelium where it meets the nonsensory epithelium. These observations demonstrate that, in the opossum VNO, there is a population of proliferating cells in the basal region close to the basal lamina in the sensory epithelium. The newly generated neurons in the basal region migrate vertically into the receptor cell layer.

Animals↗
PubMed1998

Segregated pathways in the vomeronasal system.

The vomeronasal system of mammals is chemoarchitecturally dichotomous. Two populations of receptor cells have been identified in the vomeronasal sensory epithelium based on the family of receptor proteins they express on their membranes. These two receptor cell populations express different G-proteins: the more basal population expresses Goalpha and the more apical population expresses Gialpha2. The Goalpha-expressing receptor cells project their axons to the posterior accessory olfactory bulb (AOB) whereas the Gialpha2-expressing cells project their axons to the anterior AOB. In all mammals studied to date, the anterior AOB is Gialpha2-positive and the posterior AOB is Goalpha-positive. These two parts of the AOB are also chemoarchitecturally heterogeneous with respect to their carbohydrate content as revealed both with lectin binding and immunoreactivity to monoclonal antibodies raised against carbohydrate moieties. However, species differences have been observed with respect to lectin binding, as with NADPH-diaphorase reactions and OMP immunoreactivity. Recent studies indicate that there are physiological and behavioral correlates to the dichotomy within the vomeronasal system.

Animals↗
PubMed1998

Heterogeneity in the accessory olfactory system.

The mammalian accessory olfactory bulb (AOB) is chemoarchitecturally heterogeneous in that it stains differentially with a number of markers; the receptor cells that project to the AOB are similarly heterogeneous. What is the significance of this heterogeneity? We have found that the AOB of the gray, short-tailed opossum, Monodelphis domestica, stains differentially with a number of 'markers': antibodies to olfactory marker protein (OMP) and the alpha subunit of the G protein Gi2, the lectin of Vicia villosa and NADPH-diaphorase. These markers stain the rostral AOB more strongly than the caudal AOB whereas, the G protein subunit G(o) alpha is located predominantly in the posterior subdivision of the AOB. This heterogeneity in the chemoarchitecture of the AOB may reflect a fundamental organizational dichotomy within the vomeronasal system that corresponds to a functional dichotomy. The vomeronasal sensory epithelium also exhibits a chemoarchitectural heterogeneity: receptor cells in the basal third are G(o) alpha-immunoreactive whereas the cells in the middle third are Gi2 alpha-immunoreactive. Tracing studies using WGA-HRP demonstrate that the neurons in the middle third of the vomeronasal sensory epithelium project their axons to the anterior AOB whereas those in the basal third appear to project to the posterior AOB.

Animals↗
PubMed1998

[The effect of ultrapure hyaluronic acid with different molecular weights on the healing of porcine full thickness skin wound].

Hyaluronic acid (HA) is an extracellular matrix having a high molecular weight. This study is to investigate (1) whether the ultrapure HA had effect on the healing of full thickness skin wounds in animals, and (2) the relation between the effect of HA and the size of the molecular weight of HA. The ultrapure HA with different molecular weights and HA with intermediate molecular weight were applied topically to the wounds from removal of full thickness of skin and topical application of normal saline was used as control. At 21st, day after the topical application, the tensile strength was reduced in wounds of groups of HA with high and intermediate molecular weights, but not in the group of low molecular weight. Using laser-Doppler scanning of the dynamic changes of the wound perfusion, in the 3rd day the blood supply to the wound was reduced in HA groups of high and intermediate molecular weights, but at the 7th day, the blood supply of all wounds in the two groups was increased. The study revealed that HA with different molecular weights had different actions on the contraction of wounds, the angiogenesis of the granulation tissues and the healing strength of the wounds. It was indicated that hyaluronan could be used for wound treatment or as a carrier, but its accurate composition must be carefully noted.

Animals↗
PubMed1998

[Association of apoB levels and nutrition of coronary heart disease patients].

Blood lipids and apo B levels were measured on 101 Coronary Heart Disease patients and 100 controls. The results showed that the apo B levels of patients were higher than that of controls. The average values were (877 +/- 129) mg/L and (800 +/- 95) mg/L respectively. It was found that there was close association among body mass index(BMI), ratio of waist circumference to hip circumference(WHR) and apo B levels. Besides, the results showed that dietary habit of "eating fat meat" might result in the increase of apo B concentration while the VC intake was negatively associated with serum apo B levels.

Adult↗
PubMed1998

[A 24-hour solid state Holter recording and analyzing system].

The design and functions of a new type of multi-channel solid-state Holter system were introduced in this paper. The flash memory card based recorder can record 24 hours or more of data from up to all 12 leads. The full function scanner software can analysis automatically and archive with complete editing capabilities.

Electrocardiography, Ambulatory↗
PubMed1997

Development of vomeronasal receptor neuron subclasses and establishment of topographic projections to the accessory olfactory bulb.

Previous studies of the adult vomeronasal system have shown that vomeronasal receptor neurons in the middle layer (expressing Gi alpha 2) and deep layers (expressing Go alpha) of the sensory epithelium project to the anterior and posterior parts of the accessory olfactory bulb (AOB), respectively. In the present study, the development of the two populations of vomeronasal receptor neurons and their segregated projections were investigated in the opossum, Monodelphis domestica. Antibodies to G proteins Gi alpha 2 and Go alpha were used to identify the two subpopulations of receptor neurons. The Gi alpha 2-immunoreactive (ir) cells and Go alpha-ir cells appeared between postnatal day 0 (P0) and postnatal day 3 (P3) and both types of cells increased in number during later development. The differential localization of Gi alpha 2-ir cells in the middle layer and Go alpha-ir cells in the deep layer of the VNO could be seen as early as P3 and became more prominent at later stages. The AOB was clearly identified at P10, and at this stage segregated projections of Gi alpha 2-ir fibers to the anterior part and Go alpha-ir fibers to the posterior part of the AOB were seen. The segregation of the two types of fibers in the AOB resemble that in the adult after P21. These results suggest that Gi alpha 2-ir and Go alpha-ir subpopulations of receptor neurons in the VNO develop in parallel, and that segregation of the two populations of receptor neurons in the VNO and the topographic projection to the AOB are established at very early stages during development.

Animals↗
PubMed1997

Segregated populations of mitral/tufted cells in the accessory olfactory bulb.

The dendritic distribution of mitral/tufted (M/T) cells in the opossum accessory olfactory bulb (AOB) was investigated using intracellular injection of Lucifer yellow and DiI labeling. Lucifer yellow labeling demonstrated that the primary dendrites of M/T cells are restricted to one of the two subregions (anterior or posterior) of the glomerular layer. When DiI was placed in the anterior or posterior subregion of the glomerular layer, virtually all of the labeled cell bodies in the AOB were located in the anterior or posterior part of the M/T cell layer, respectively. These results demonstrate that the anterior and posterior subregions of the AOB glomerular layer are termination sites for dendrites belonging to distinct populations of M/T cells.

Animals↗
PubMed1996

Subclasses of vomeronasal receptor neurons: differential expression of G proteins (Gi alpha 2 and G(o alpha)) and segregated projections to the accessory olfactory bulb.

Differential expression of G proteins (Gi alpha 2 and G(o alpha) and the separate central projections of Gi alpha 2- and G(o alpha)-immunoreactive (ir) vomeronasal receptor neurons were investigated in the mouse and rat using immunocytochemical methods. In the vomeronasal organ (VNO), receptor neurons with their cell bodies located in the middle layer (middle 1/3) of the vomeronasal sensory epithelium express Gi alpha 2. Axons of these Gi alpha 2-ir neurons can be followed from VNO to the anterior part, but not the posterior part, of the nerve-glomerular (N-GL) layer of the accessory olfactory bulb (AOB). Another population of receptor neurons, which are located in the deep layer (basal 1/3) of the vomeronasal sensory epithelium, express G(o alpha), and axons of the G(o alpha)-ir neurons can be traced to the posterior part, but not the anterior part, of the N-GL layers of the AOB. The axons of the two subclasses of receptor neurons are intermingled near the VNO and become segregated as they enter the AOB. Removal of the AOB results in retrograde degeneration of both Gi alpha 2-ir and G(o alpha)-ir receptor neurons in the VNO. These results suggest that at least two subclasses of receptor neurons exist in the VNO: the Gi alpha 2-ir neurons in the middle layer and the G(o alpha)-ir neurons in the deep layer of the VNO. The Gi alpha 2-ir neurons in the middle layer of the VNO project to the anterior part of the AOB, while the G(o alpha)-ir neurons in the deep layer of the VNO project to the posterior half of the AOB. These results are similar to our previous observations in the gray short-tailed opossum, suggesting that the existence of at least two subclasses of receptor neurons in the vomeronasal epithelium with differential projections to the AOB is a conserved feature among mammals.

Animals↗
PubMed1996

[Prediction of skin flap viability by laser Doppler scanning].

Twelve female pigs were used for the experiment. An island flap (8 cm x 15 cm) pedicled with the superficial circumflex iliac vessels was elevated on the flank of the pig. The blood flow of the flap was measured with laser doppler scanning at different times, i.e. before and after flap elevation; before clipping the feeding artery of the flap and 9 hours after clipping the artery; 5 minutes, 20 minutes, 40 minutes, 1 hour and 40 hours after reperfusion. The flap viability was also examined with naked eye and fluorography. Biopsy examinations were used as gold standard of flap viability. The results showed that predicting accuracy of flap viability by naked eye was 76%; fluorography was 94% and laser doppler scanning was 91.3%. No significant difference was found between the results from fluorography and laser doppler scanning while there was significant difference between naked eye and laser doppler scanning. The authors discussed the clinical applications of predicting skin flap viability.

Animals↗