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Biomedical subjects

C Jersild

Publications and source records attributed to C Jersild.

At least 91 records · Page 5Linked to original sources
PubMed1976

Immunological in vitro parameters in patients with multiple sclerosis and in normal individuals.

The general immunological capacity of 40 patients with multiple sclerosis has been evaluated with lymphocyte transformation test including both mitogens (PHA and PWM) and antigens (PPD, Candida albicans, Staph. aureus and E. coli). Determination of T and B cells was performed by E-, EAC-rosetting and immunofluorescence for surface immunoglobulins. Compared with the results obtained in 42 normal individuals only minor differences were found.

Adult↗
PubMed1975

Mixed lymphocyte culture determinants and C2 deficiency: LD-7a associated with C2 deficiency in four families.

Four families with C2 deficiency were studied. Among eight HL-A haplotypes involved with C2 deficiency, five were HL-A 10,W18. Three homozygotes for C2 deficiency from different families were mutually nonreactive in mixed lymphocyte cultures (MLC) and the heterozygotes from the fourth family failed to react to the homozygous cells. It appeared that identical MLC determinants were associated with all the genes from the different families that related to C2 deficiency. Further experiments identified the MLC determinant, LD-7a, as being involved. These results suggest marked linkage disequilibrium between the genes for C2 deficiency and the major histocompatibility complex (MHC). Studies of possible recombinants have offered tentative evidence for the positioning of the locus for C2 deficiency with respect to other segments of the MHC.

Chromosome Mapping↗
PubMed1975

Hereditary C2 deficiency: Genetic studies and association with the HL-A system.

Herediatary C2-deficiency has been shown to be transmitted asn an autosomal recessive characteristic. Recent evidence indicates that some genetic factors involved in the control of the complement (C) system in both man and mice are governed by genes localized within the major histocompatibility regionmthis study describes a large pedigree of the paternal family of a C2-deficient patient with systemic lupus erythematosusl It is shown that this condition is transmitted as an autosomal recessive trait, the heterozygous carriers having approximately half normal levels of C2. Furthermore, this trait was shown to be inherited in close linkage with an infrequent HL-A typw, 2,4A2. The maternal, C2-defective chromosome was shown to be transmitted by HL-AW10, W18 haplotypemthis same haplotype was described in a similar study by Fu et al. (6) to be associated with C2 deficiencymfinally, a third haplotype HL-A2,W18 carrying a defective C2 gene was demonstrated in a part of this pedigree.

Complement C2↗
PubMed1975

Recognition by pregnancy serums of non-HL-A alloantigens selectively expressed on B lymphocytes.

A group of alloantibodies are found in pregnancy sera which react with antigens present on B lymphocytes and monocytes but are not detectable on the vast majority of unstimulated T cells. This specificity distinguishes them from HL-A antibodies which react with both cell types. They were readily recognized through indirect fluorescent antibody analysis by employing the combination of B-cell lymphoid lines and normal peripheral blood T cells. Different sera gave a variety of patterns of reactivity with a panel of 11 lymphoid lines. Similar differential patterns were also observed with normal B cells from different individuals particularly after concentrating the B cells. The antibodies were also cytotoxic to B cells and this procedure gave parallel results to the fluorescence method. The pattern of reactions obtained indicated a very heterogeneous system similar to that for HL-A. Special study of certain of the sera provided evidence that the lymphocyte-defined determinants of the mixed lymphocyte reaction system were involved. For convenience the term HL-B has been employed for these antigens.

Antibody Specificity↗
PubMed1975

Evidence for tryosine peptide homologies in the HLA antigens system.

The tetrameric HLA antigens are composed of two heavier chains which carry the alloantigenic determinants and two lighter chains identified as beta2-microglobulin. Although at least 40 different antisera are required to define the varying HLA specificities, it appears that these antigens may be closely related to each other and to the immunoglobulins. Through the use of a new electrophoretic technique, which is able to compare simultaneously the tyrosine peptides produced from radioiodinated cell surface proteins, this report gives evidence that HLA antigens of the three chromosomal loci may have similar amino-acid sequences. Since the retention of homologous tyrosine residues and a tendency for sequence preservation surrounding these residues are features of immunoglobulin structure, this may indicate that similarly conservative evolutionary mechanisms have been operative in the HLA allelelic proteins or that immunoglobulins and HLA antigens may indeed have a common evolutionary origin.

Antigen-Antibody Reactions↗
PubMed1975

Detection of measles antibodies in cerebrospinal fluid and serum by a radioimmunoassay.

Evidence that different structural components of the measles virus may act as antigens has been provided by the serologic methods of hemagglutination inhibition hemolysin inhibition, and nucleocapsid complement fixation. Using radioiodinated measles viral antigens, and immune precipitation assay has been designed that is capable of discriminating among various reactivities to measles viral structural components in serum or cerebrospinal fluid (CSF) and of distinguishing whether IgG and IgM antibody is involved. This technique has been applied to the study of measles antibodies in CSF and sera of patients with multiple sclerosis (MS) and other neurologic diseases. From data presented here, it was found that both groups of patients have individual reactivity to measles proteins, present in CSF and serum, whereas three normal CSF samples were found not to have such antibodies. It appears that oligoclonal immunoglobulins in CSF of MS patients may be detected by this method, and one patient with MS was found to have CSF IgM anti-measles antibodies.

Antibodies, Viral↗
PubMed1975

HL-A antigen, gene, and haplotype frequencies in Denmark.

Between 426 and 1,967 unrelated Danes have been HL-A typed for most presently known HL-A antigens of the LA (first), FOUR (second), and AJ (third) segregant series. Antigen, gene and haplotype frequencies with delta values are given. AJ series antigens are most strongly associated with some of the FOUR series antigens, and except for one case, the linkage disequilibrium between AJ and FOUR does not seem to be influenced by the LA series; the exception concerns HL-A9, RH-315, and 12: the RH-315 determinant is significantly more frequent on HL-A9, 12 haplotypes and on other HL-A12 carrying haplotypes. The term "superhaplotype" is suggested for gene constellations such as the HL-A9, RH-315, 12 "haplotype". It is suggested that the associations between cross-reacting antigens from one series with the same antigen from another series may reflect recent evolutionary divergence of the cross-reacting antigens.

Chromosome Mapping↗
PubMed1974

Two separate genes controlling stimulation in mixed lymphocyte reaction in man.

The genetic control of strong stimulation in the mixed lymphocyte culture reaction is determined by a separate gene (MLR-S) closely linked to the FOUR-locus of the HL-A chromosomal region. Three additional examples of siblings with recombination between FOUR-locus and MLR-S locus are presented which confirms the independent genetic control of mixed lymphocyte reaction from the control of HL-A antigens. The occurrence of two recombinant children in one family with four other children representing all possible HL-A haplotype-combinations, strongly supports the genetic mapping of the MLR-S determinants outside the HL-A chromosomal region. The experiments presented show that additional genes located within the HL-A region itself contribute with a weak stimulation of allogenic mixtures. These data are discussed in relation to the marginal stimulation of the mixed lymphocyte culture reaction which can be seen between unrelated individuals. It seems that a group of relatively histocompatible individuals can be defined by identity of the MLR-S locus but with differences on the weak MLC-determinants, and that this group for the purpose of clinical transplantation behaves as histocompatible individuals.

Adult↗