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Biomedical subjects

C Jensen

Publications and source records attributed to C Jensen.

At least 109 records · Page 6Linked to original sources

Endotoxins release histamine by complement activation and potentiate bacteria-induced histamine release.

The histamine-releasing capability of bacterial lipopolysaccharides (LPS) was examined in human leukocyte suspensions. LPS alone did not release histamine, but it was found to enhance the histamine release caused by bacteria in basophils from persons sensitized to these bacteria. In the presence of serum, LPS was able to release histamine through complement activation. It is speculated that endotoxins reinforce release of histamine caused by bacteria in persons sensitized to these microorganisms, and a direct mediator release via complement activation might play a role in septic conditions.

Bacteria↗

Absorption of individual fatty acids from long chain or medium chain triglycerides in very small infants.

Using stool samples previously obtained for a nutritional balance study of very small infants, the intake, excretion, and percent absorption (%A) of individual fatty acids were quantitated by gas-liquid chromatography in two groups of patients; one receiving a formula providing fat as long chain triglycerides (LCT), the other providing 40% fat as medium chain triglyceride (MCT). The total fat intake for each group was 7.22 g/kg/day (MCT group), 6.02 g/kg/day (LCT group) and the absorption was 6.79 g/kg/day (MCT group) and 5.41 g/kg/day (LCT group). The %A of individual acids was comparable in both groups being greater than 99% for MCT and being lowest (62%) for C18:0 FA. As a consequence, infants in the MCT-fed group had a better total %A of fat, 95.2 vs 89.9% and absorbed more fat than the LCT group.

Chromatography, Gas↗

Influence of bacterial endotoxins on basophil histamine release. Potentiation of antigen- and bacteria-induced histamine release.

The histamine-releasing capability of lipopolysaccharides (LPS) was examined in human leukocyte suspensions. LPS alone did not release histamine, but was found to enhance the histamine release caused by anti-IgE. Also the IgE-mediated histamine release caused by specific antigens (allergens or bacteria) in sensitized individuals was enhanced by LPS. The potentiating effect of LPS was observed in grass pollen and dog dander allergic patients as well as in patients sensitized to E. coli or Staph. aureus bacteria. No potentiation was obtained by exposure to unspecific allergens or bacteria to which the persons were not sensitized. Bacteria can release histamine by immunological or nonimmunological mechanisms, and only the immunological histamine release was found to be potentiated by LPS. It is speculated that endotoxins reinforce release of histamine caused by allergens in allergic patients or by bacteria in persons sensitized to these microorganisms.

Allergens↗

Influence of neuraminidase and N-acetylneuraminic acid on basophil histamine release in vitro.

Since N-acetylneuraminic acid (NANA) in cell membrane glucocalyx mediates or modulates a variety of actions, such as mediator release, we examined a possible modulating role of this amino sugar in histamine release from human basophil leukocytes. Removal of NANA from the cell membrane by the enzyme neuraminidase caused a dose-dependent histamine release. Removal of smaller amounts of NANA enhanced histamine release induced by anti-IgE, Concanavalin A and the calcium ionophore A23187, and reduced the interval between addition of antigen and initiation of histamine release. Pretreatment with free NANA had the opposite effects, i.e. a diminished and delayed maximal histamine release. The hypothesis that NANA in the cell membrane modulates the cellular response to stimulation was further substantiated by demonstrating that the altered response was reflected by a change in the sensitivity of the cell to extracellular calcium. NANA in the cell membrane glucocalyx thus seems to modulate the basophil response to stimulation by modulating transmembraneous calcium transport.

Adolescent↗

Basophil histamine release in cord blood. Regulatory role of IgE.

Thirty-two cord blood samples were studied for histamine releasing capability by using a sensitive glass microfibre-based histamine analysis. Histamine was obtained after challenge with anti-IgE in 24 of the 32 samples. However, the net release in cord blood was only 25% of that in adult blood and no relationship was found between histamine release response, total IgE in cord plasma, and a family history of atopic diseases. The low histamine release in cord blood seemed to be associated with the immunological IgE receptor complex activation and not with an immature basic cell function, since the calcium ionophore A23187 which bypasses the receptor complex induced identical histamine release curves in cord and adult blood. Furthermore, when comparing the results of passive sensitization of basophils from new-born and adult persons, the new-born basophils possessed a significant fraction of free IgE receptors, whereas in adults most of the receptors were occupied by IgE.

Adult↗

Bacteria and their products release histamine and potentiate mediator release: new aspects in airway diseases.

The possibility that bacteria and their products play a role in airway diseases, due to their capacity to release histamine, was investigated in blood leucocytes from patients suffering from respiratory diseases and from healthy individuals. The results clearly show that bacteria release histamine by immunological and non-immunological mechanisms and that endotoxins enhance mediator release caused by allergens in allergic patients or by bacteria in persons sensitized to these organisms. Furthermore, a great increase is obtained in mediator release by the combination of immunological and non-immunological mechanisms.

Adult↗

Serum-cholesterol-lowering effect of metronidazole and possible mechanisms of action.

In five patients with Crohn's disease long-term therapy with metronidazole (400 mg b.i.d.) was followed by a significant reduction of total serum cholesterol from 179 mg/dl to 156 mg/dl, 134 mg/dl, and 143 mg/dl, after 2-4 months, 6 months, and 9-12 months, respectively. Lipoprotein analysis before and after 3 weeks of administration of metronidazole (400 mg/day) to five normolipemic volunteers revealed that LDL-cholesterol was reduced by 21% (P less than 0.05), whereas HDL-cholesterol remained unchanged. Biliary secretion of cholesterol and bile acids were reduced by 13% and 20% (P less than 0.05), respectively, which might suggest a decreased sterol synthesis. The amount and percentage of intestinal cholesterol absorption were decreased by 33% and 22% (P less than 0.05). Thus, a possible decrease in sterol synthesis and a reduction of cholesterol absorption might be responsible for the serum-cholesterol-lowering effect of metronidazole. However, caution should be taken when considering metronidazole for long-term treatment of patients with hypercholesterolemia due to possible side effects.

Bile↗

Bacteria-induced histamine release. Examination of the bacterial cell wall components peptidoglycan, teichoic acid and protein A.

The histamine-releasing capability of whole bacteria was examined in leukocyte suspensions from normal individuals. Both gram-positive and gram-negative bacteria caused basophil histamine release. It is probably the bacterial cell wall which interacts with the basophil cell surface leading to release of histamine, since cell walls showed higher histamine releasing capability than the whole bacteria. The releasing effect of the bacterial cell wall components peptidoglycan, teichoic acid and protein A was examined. The peptidoglycan preparations were found to be more potent than the corresponding whole bacteria and cell walls. Since peptidoglycan is found in the cell wall of both gram-positive and gram-negative bacteria, it might be a common factor responsible for histamine release by different bacteria. No release was obtained by teichoic acid, whereas protein A caused histamine release in leukocytes from allergic patients, but only a poor release in normal individuals. The initial step in protein A-induced histamine release might be a binding of protein A to IgE on the cell surface, since removal of cell-bound IgE reduced the release and a high correlation was found between protein A- and anti-IgE-induced histamine release.

Bacterial Physiological Phenomena↗

Objective comparison of physical dysfunction after neck dissection.

Thirty-five patients who underwent a total of 44 neck dissections of various types were prospectively studied to compare differences in postoperative shoulder function. Those who underwent a radical neck dissection suffered the greatest reduction in shoulder movement and had severely abnormal electromyograms. Those who underwent modified neck dissection with preservation of the spinal accessory nerve suffered less loss of shoulder function than the radical neck dissection group, but not to a significant degree at 16 weeks; however, the electromyograms of patients who underwent modified neck dissection were significantly better than those of the radical neck dissection group, which suggests that these patients may improve with time. Indeed, a reevaluation of several patients at 1 year showed improvement in both shoulder function and electromyograms in those who underwent modified neck dissection. Patients who underwent supraomohyoid neck dissection that involved minimal dissection of the spinal accessory nerve had minimal loss of shoulder function and usually, normal electromyograms at 16 weeks that documented less injury to the spinal accessory nerve. Again, these patients had improvement with time. A correlational analysis revealed that the physical parameters correlated well with the electromyographic findings, whereas each patient's perception of disability did not. These findings suggest that, in patients in whom it is oncologically sound, a neck dissection that spares the spinal accessory nerve offers significant benefit in terms of shoulder function.

Accessory Nerve↗

Prevention of lesioning-induced precocious puberty in female rats by neonatal implantation of fetal preoptic tissue.

Damage to the medial preoptic area (MPOA) was produced in one-day-old female rats by bilateral electrolytic lesions or by aspiration of preoptic tissue. Both procedures resulted in similar advancement of vaginal opening and the first ovulation. A further group of rats was lesioned or aspirated in the MPOA and immediately bilaterally implanted with medial preoptic tissue collected by the puncture method from 17-19-day-old female rat fetuses. After autopsy, tissue destruction and implants located in the MPOA could be identified in 5 females that showed significantly later onset of puberty than both the lesioned or aspirated and the untreated controls. The findings suggest that the elimination of neurones and not a stimulatory effect of electrolytic lesions on GnRH secretion is responsible for the acceleration of sexual maturation recorded after lesioning of the MPOA. Possible reasons for the failure to identify the grafts in most of the implanted females are discussed.

Animals↗

Component probability and component reinforcer rate as biasers of free-operant detection.

Six pigeons were trained on multiple schedules whose components were concurrent variable-interval extinction and concurrent extinction variable-interval schedules. In Experiments 1a and 1b the stimuli signaling the components were two different light intensities, and in Experiments 2a and 2b they were two identical intensities. The components of the multiple schedule changed probabilistically after each reinforcer. In Experiments 1a and 2a, the probability of presenting the components was varied over five conditions and a replication. In Experiments 1b and 2b, the component probability was .5 and the component reinforcer rates were varied systematically over five conditions and a replication. The data, analyzed according to the Davison-Tustin behavioral detection model, confirmed that the discriminability of the stimuli signaling the components was high when the stimuli were different, and low when the stimuli were the same. Discriminability, measured by log d, was unaffected by component probability variation and by component reinforcer-rate variation. When discriminability was high, bias, or the response allocation between the two keys, was more strongly affected by variation of reinforcer rate within components than by variation of component probability, but the reverse was found when discriminability was low. The results suggest that free-operant detection performance is controlled by the rates of reinforcers in periods of time in which stimuli signal differential contingencies. These periods comprise the components when the component stimuli are discriminable, and comprise the total session when the components are indiscriminable. An extension of the Davison-Tustin behavioral detection model that incorporates these results is presented.

Journal Article↗

Purulent pericarditis in children. Review and case report.

Acute purulent pericarditis is infrequent in children. Only around 200 cases have been reported in the western literature in the past 25 years (1-10). From the Scandinavian countries only two cases have been described (6, 7). Purulent pericarditis is usually a complication of upper respiratory tract infection. Mortality is very high in unrecognized or inadequately treated cases. Early diagnosis and appropriate treatment with adequate surgical drainage and antimicrobial therapy are vitally important for the outcome.

Child, Preschool↗

Altered expression of the H+ ATPase in Streptococcus faecalis membranes.

Evidence is presented that expression of the H+ ATPase in S. faecalis is influenced by the extracellular pH and K+ level during growth. Altered expression was detected by assay of F1 ATPase and electrophoretic analysis of membrane proteins. K+-limited growth caused about a 2-fold increase in the F1 ATPase. The effect of growth at pH 6, 7 and 9 was studied. Compared to cells grown at pH 7, growth at pH 6 increased the F1 ATPase about 2-fold while growth at pH 9 reduced the F1 ATPase by nearly 4-fold. The elevated F1 ATPase activity in the pH 6 cells was associated with an increase in the F1 ATPase alpha and beta subunits in the membrane while the decrease in F1 ATPase in the pH 9 cells was associated with a marked loss of the alpha subunit. It is suggested that intracellular protons may act as effectors which regulate expression of the F1F0 gene cluster at the level of translation.

Electrophoresis, Polyacrylamide Gel↗

Lectin-mediated reactions in histamine release caused by bacteria.

The bacteria-induced release of histamine was studied in human basophil leukocytes and in isolated rat mast cells. Whole bacteria of Staph. aureus caused release in a 98% pure population of peritoneal mast cells from germ-free rats, indicating a non-immunological mechanism and a direct interaction between the bacteria and the target cells. Probably the bacterial cell wall interacts with the cell membrane, since a preparation of the bacterial cell wall caused a dose-dependent release of histamine from basophil leukocytes similar to that induced by whole bacteria, and repeated washing of whole bacteria did not change the release. Inhibition studies by lectin-binding sugars indicate that aminosugars on the bacterial surface of Staph. aureus interact with lectins on the basophil cell membrane leading to histamine release.

Adult↗