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Biomedical subjects

C J Weber

Publications and source records attributed to C J Weber.

At least 55 records · Page 3Linked to original sources
PubMed1983

Rapid detection of clindamycin resistance in Bacteroides spp.

High-level resistance to clindamycin can be accurately detected by the Wadsworth disk identification test. Of the 98 isolates of the Bacteroides fragilis group that were tested, 90 were inhibited by the 60-micrograms erythromycin disk and had clindamycin minimal inhibitory concentrations of less than or equal to 3.2 micrograms/ml. Of the remaining eight isolates, all were resistant to the erythromycin disk and had clindamycin minimal inhibitory concentrations of greater than or equal to 100 micrograms/ml.

Bacteroides fragilis↗
PubMed1980

Alternatives in pancreatic islet transplantation: tissue culture studies.

Culture of islets isolated from the pancreas of neonatal rats successfully preserved function for 6 days, as indicated by release of insulin into the culture medium and reversal of experimental diabetes bu cultured islet isografts. Release of insulin into the culture medium correlated with lowering of fasting blood glucose in the diabetic recipient. Intravenous glucose tolerance was normal in recipients of immediate and 24-h cultured islets but abnormal in recipients of 6-day cultured islets. Culture of the islets isolated from human pancreases (cadaveric) resulted in islet purification but at the expense of loss of islets, with only 1--5% surviving 1 wk of culture. During 8--10 days in culture, insulin release declined, and histological examination revealed only a few viable islets. Islets isolated from surgical specimens survived the culture conditions as demonstrated by viable cell clumps and insulin secretion. After an 85% pancreatectomy in the patient with chronic pancreatitis, islets were isolated, cultured for 7 days, and then autografted into muscle pockets and the subcutaneous space of the forearm. There was no evidence of function.

Animals↗
PubMed1979

Effect of vasopressin on vascular smooth muscle from cold- and warm-acclimated rabbits.

Contractile properties of helical auricular arterial strips from warm- and cold-acclimated (WA and CA) rabbits were studied to determine whether vascular smooth muscle shows a cold-induced alteration in response to vasopressen (Vp), similar to the effect seen with catecholamines. The CA response was decreased at all Vp concentrations tested and the decreases were significant at doses between 0.4--100.0 mUnits Vp/ml. Results are discussed in relation to the altered renal tubular response to Vp which has previously been reported to occur in cold-acclimated animals.

Acclimatization↗
PubMed1978

Tissue culture preservation and intramuscular transplantation of pancreatic islets.

Pancreatic tissue from neonatal rats was cultured for 6 days and then transplanted into isologous animals made diabetic with streptozotocin. The cultured pancreatic tissue was implanted either into the peritoneal cavity or into muscle pockets and showed survival by functional and morphological studies for longer than 40 weeks. Pancreatic tissue from a patient subjected to subtotal pancreatectomy for chronic pancreatitis was maintained in culture for 7 days, with evidence for insulin release into medium, sterility, and histological viability of islets. Autotransplantation of this tissue into muscle pockets of the forearm was tolerated well. Glucose tolerance improved following transplantation, but evidence for function of the graft (insulin, C-peptide) remains inconclusive, 4 months after transplantation.

Animals↗
PubMed1977

Tissue culture isolation and preservation of human cadaveric pancreatic islets.

Six specimens of human cadaveric pancreas were collagenase-dispersed and placed in tissue culture. Media were changed at 2 day intervals and assayed for insulin and amylase. Experiments were terminated after 8 days, and phase-contrast and light microscopy were performed on cultured tissue. Insulin content in media remained high for 6 days in five cases and for 8 days in four experiments; this correlated directly with morphologic viability of cultured tissue. Media amylase fell to zero after 4 days in four cases and after 6 days in two cases. These data support the concept that tissue culture may be an efficient method for (1) islet cell purification, since acinar tissue and amylase activity disappear, and (2) islet preservation.

Adult↗
PubMed1976

Factors influencing patency of femoropopliteal artery bypass grafts.

An analysis of 276 femoropopliteal bypass procedures performed in 264 patients at the Columbia-Presbyterian Medical Center over the past two decades showed a direct relationship of graft patency to preoperative popliteal artery runoff. Fuctional results were better than patency results. Sympathectomy and anticoagulation did not improve graft patency. The risk of amputation is outweighed by the benefits of restoration of blood flow to the ischemic extremity by a byass procedure.

Amputation, Surgical↗
PubMed1976

Hyperinsulinemia and hyperglucagonemia following pancreatic islet transplantation in diabetic rats.

Fasting blood glucose (FBG), serum immunoreactive insulin (IRI), plasma immunoreactive glucagon (IRG), body weight, and caloric intake were measured in long-term islet-isografted rats eight to 10 months following intraperitoneal islet transplantation in in age-matched, sham-operated, concurrently followed normal and diabetic controls. Islet recipients had normal body weights, but they were significantly polyphagic, hyperinsulinemic, and hyperglucagonemic when compared with normals. Fasting blood glucose levels were reduced by 10 per cent. Several factors may be related to the occurrence of these abnormalities in long-term islet-isografted rats, including (1) the mass of islets transplanted, (2) the age of donor tissue, (3) the heterotopic location of islet grafts, and (4) the lack of normal innervation of transplanted islet cells.

Age Factors↗