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C J Palenik

Publications and source records attributed to C J Palenik.

At least 19 recordsLinked to original sources
PubMed1994

Effectiveness of two types of sterilizers on the contents of sharps containers.

The purpose of this study was to evaluate the killing effect that a gravity steam autoclave (GSA), a high-vacuum steam sterilizer (HVA) or an unsaturated chemical vapor sterilizer (UCV) had on endospores present on strips or applied to dental needles within three sizes of sharps containers. Commercial spore strips containing 1.7 x 10(5) Bacillus stearothermophilus endospores were used, while the needles were soiled with an equal number of spores or with spores mixed with blood. Needles were tested capped and uncapped. Initial operating conditions were for the: (1) GSA were 129 kPa at 124 degrees C for 30 minutes; (2) HVA were 214 kPa at 131 degrees C for 10 minutes and (3) UCV, 172 kPa at 134 degrees C for 20 minutes. Strips and needles were placed within 3/4 filled containers. Fill materials consisted of needles, syringes, plastic sheaths and scalpel blades. Containers were processed upward or on their sides. Twenty-five strips or needles comprised a test group for each operational parameter. Processed strips and needles were aerobically cultured at 56 degrees C for 7 days. If sterilization was not accomplished after the initial period, additional exposure time was added. Results included: (1) soiled needles are more difficult than strips to sterilize; (2) capping or the presence of blood did not affect sterilization efficiency; (3) container positioning was important only for the smallest container; (4) additional exposure time was usually required when sterilizing soiled needles; (5) the HVA killed all spore challenges in a single sterilization cycle and (6) GSA and UCV were approximately equal in sterilization efficiency.(ABSTRACT TRUNCATED AT 250 WORDS)

Anesthesia, Dental↗
PubMed1992

Inhibition of microbial adherence and growth by various glass ionomers in vitro.

This study measured the in vitro inhibition of growth and adherence of five oral bacteria by glass-ionomer materials. Disks were prepared from two cavity liners and four restorative class materials, by use of Teflon plates with circular wells, five mm wide and two mm deep. The bacterial species tested included: A. viscosus, S. mitis, S. mutans, L. casei, and S. sanguis. Growth inhibition studies were performed by the spreading of 0.1 mL of standardized inocula over agar plates produced with selective media, followed by the direct application of glass-ionomer disks onto the agar. On other plates, disks were placed onto uninoculated agars for 48 h, followed by bacterial inoculation. All agar plates were incubated under optimal growth conditions for each bacterial species. The four restorative materials were also placed aseptically into sterilized bovine incisors and placed into sucrose containing broth media, inoculated with S. mutans for three days. Adhering materials were disclosed and scored. An ion-exchange electrode was used to measure fluoride release over a seven-day period for all six glass ionomers. The two cavity liners and two of the restorative materials produced the largest growth inhibition zones by direct contact. No growth inhibition occurred when the specimens were allowed to come into contact with the agars prior to inoculation. All four restorative materials reduced bacterial accumulations on enamel surfaces by over 80%. Elevations in short-term fluoride release levels were positively correlated with growth inhibition.

Actinomyces viscosus↗
PubMed1990

Effectiveness of steam autoclaving on the contents of sharps containers.

The effectiveness of steam autoclaving on bacterial endospores placed within five types of sharps containers was tested. A variety of container physical orientations within the autoclave were evaluated. Spores were present on commercial spore strips or placed onto capped and uncapped dental needles. All strips and needles present in empty or filled containers could be sterilized within 15 minutes when the containers were placed on their sides and their vents left open. The contents of containers processed in an upward position required between 30-60 minutes of autoclaving before being sterilized. The size and shape of the containers influenced ease of sterilization.

Dental Instruments↗
PubMed1988

AIDS in Indiana.

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Acquired Immunodeficiency Syndrome↗