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Biomedical subjects

C J Leake

Publications and source records attributed to C J Leake.

28 records · Page 2Linked to original sources

An outbreak of type 2 dengue fever in the Seychelles, probably transmitted by Aedes albopictus (Skuse).

Between December 1976 and September 1977 the Seychelles group of islands in the Indian Ocean was struck by an extensive epidemic of dengue fever. The peak of the epidemic was in the last week of February. Type 2 dengue virus was isolated from patients and mosquitos. Aedes albopictus was the sole vector. The clinical picture was that of classical dengue. Haemorrhagic fever and the shock syndrome were not observed.Absenteeism from schools and offices, anamnestic questioning, and prevalence of antibodies in sera collected after the epidemic was over, indicated that approximately 75% of the population had been infected. Serological evidence was obtained of an epidemic of dengue in the islands more than 40 years earlier. This was confirmed by archival records.

Adolescent↗

Investigation of the ecology of Soldado virus on Puffin Island, North Wales.

The prevalence of Soldado (SOL) virus and SOL virus antibodies was investigated on immature sea birds and the argasid tick Ornithodoros (Alectorobius) maritimus collected on Puffin Island, North Wales. No SOL virus was recovered from 133 bird sera, but 2 of the birds exhibited neutralizing antibodies against SOL virus. Nine of 27 tick pools (226 individuals) and 34 of 173 ticks tested individually proved to be infected with SOL virus.

Animals↗

Cytopathic effect and plaque formation by arboviruses in a continuous cell line (XTC-2) from the toad Xenopus laevis.

Forty-six arboviruses were tested for c.p.e. and/or plaque formation in an amphibian cell line. C.p.e. was observed with a high proportion of the viruses tested. Comparative plaque assay, in the XTC-2 cells at 28 degrees C and Vero cells at 37 degrees C, suggests that these systems are comparable in sensitivity and susceptibility to infection. Practical uses of this cell line are discussed.

Animals↗

Isolations in a mosquito (Aedes pseudoscutellaris) cell line (Mos. 61) of yellow fever virus strains from original field material.

A simple, rapid and inexpensive method of isolating yellow fever (YF) virus from naturally infected mosquitoes, human liver and the serum of a sentinel monkey by inoculation of a continuous line of mosquito cells is described. The mosquito cells were more sensitive than suckling mice and marginally better than Vero cells for primary isolation. This is the first time that mosquito cells have been successfully used for primary isolation of YF virus from field material.

Aedes↗