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Biomedical subjects

C J Issel

Publications and source records attributed to C J Issel.

95 records · Page 6Linked to original sources
PubMed1970

Serologic evidence of bunyamwera group arbovirus infections in Wisconsin and texas deer.

Sera of 81 white-tailed deer from south Texas and 283 white-tailed deer from Wisconsin were tested for neutralizing and hemagglutination-inhibiting antibodies to arboviruses of the Bunyamwera group. Neutralizing antibodies were detected in 100% of the Texas deer sera and hemagglutination-inhibiting antibodies were detected in 61% of those sera collected in 1969 and 78% of those sera collected in 1963. The prevalence of both neutralizing and hemagglutination-inhibiting antibodies in Wisconsin deer sera varied from 72-100% and 42-79% respectively depending on the geographic area tested in the years 1963 and 1969.

Animals↗
PubMedDate not supplied

Lentivirus antigen purification and characterization: isolation of equine infectious anemia virus gag and env proteins in one step by reverse phase HPLC and application to human immunodeficiency virus glycoproteins.

We describe here a one step HPLC technique for purifying the four gag proteins (p26, p15, p11 and p9) and two env glycoproteins (gp90 and gp45) from purified equine infectious anemia virus (EIAV), a member of the lentivirus subfamily of retroviruses. The purification procedure employs a reverse-phase phenyl Radial-pak cartridge contained in a high pressure radial compression chamber in which a shallow, multistep acetonitrile gradient is applied at ambient temperatures. The purified proteins are recovered at an efficiency of 60-70%. Moreover, the isolated components retain their antigenicity and are suitable for a variety of biochemical analyses including protein sequencing. The purification of EIAV gp90 and gp45 represents the first successful isolation of a lentivirus glycoprotein from purified virus preparations. The availability of these separated proteins permitted direct protein sequencing which confirmed the previously reported env gene sequence and provides important antigens for the development of diagnostic immunoassays and subunit vaccines. The procedures described appear applicable to other lentiviruses, including human immunodeficiency virus (HIV), and perhaps to hydrophobic membrane proteins in general.

Antigens, Viral↗