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Biomedical subjects

C J Herman

Publications and source records attributed to C J Herman.

At least 91 records · Page 5Linked to original sources
PubMed1979

Bone marrow fibre production in myelofibrosis: a quantitative study.

A digital image processing technique has been used to quantitate bone marrow fibre in histological material (both biopsy and necropsy) from 17 cases of myelofibrosis. An attempt was made to correlate the results of these measurements with other diagnostically relevant parameters. Bone marrow fibre was found to correlate significantly with absolute neutrophil count, the absolute count of immature granulocytic cells and with the number of immature granulocytic cells expressed as a percentage of the total leucocyte count. There was no significant correlation between marrow fibre content and platelet count for the series as a whole, but for those cases in which measurements were made following splenectomy these parameters were highly correlated. Bone marrow fibre content did not appear to correlate with liver or spleen weight at necropsy, nor with the duration of clinical manifestations of the disease. The significance of these findings is discussed.

Bone Marrow↗
PubMed1979

Immunofluorescence of histone H1 in stimulated lymphocytes measured by flow cytophotometry.

The modification of histones or their redistribution during the transition from actively transcribing chromatin to the heterochromatic chromosomes seems to play a major in regulation of gene expression. The purpose of this study was to monitor the change in immunofluorescence of histone HI during phytohemagglutinin stimulation in peripheral lymphocytes. The histone antigens were prepared from pig thymus, proven to be pure by gel electrophoresis and repeatedly injected as RNA-complexes into rabbits. The antihistone HI antiserum titer was 1:4000, and there was no cross-reactivity with other histone fractions as shown by microcomplement fixation tests. Affinity chromatography purified antibody after being labeled with fluorescein isothiocyanate was able to differentially stain HeLa cells as controls and those, where histone HI had been extracted by perchloric acid treatment. The measurements were done on a Los Alamos Scientific Laboratories-flow cytophotometer cell sorter. Staining peripheral lymphocytes resulted in a bimodal distribution. The increase in number of cells with high fluorescence intensity had its maximum about 20 hr before the maximum proliferative activity of the lymphocytes as measured by number of cells in S phase with the DNA-stain mithramycin.

Cell Cycle↗
PubMed1979

Automated cervical cytology screening: performance requirements and instrument evaluation.

Continuing progress towards automation of cervical cancer screening requires that criteria for clinical acceptability of automated systems be defined, and that methods be devised for effectively evaluating new technology. The potential roles of automation in cervical cancer detection, performance requirements, instrument evaluation and useful contributions from tuberculosis screening, automated white blood cell differential counting, signal detection theory and decision theory are discussed.

Automation↗
PubMed1976

Flow microfluorometric system for screening gynecologic cytology specimens using propidium iodide-fluorescein isothiocyanate.

Seventy cervical cytology specimens have been screened by a xero resolution flow analyzer-sorter using propidium iodide and fluorescein isothiocyanate as fluorochromes for nucleus and cytoplasm, respectively. This system shows a 1% sensitivity for detection of abnormal cells using only crude visual data analysis. Screening of clinical specimens was performed on the instrument with a 5.8% false negative rate and a 11.8% false positive rate by comparison with routine visual cytologic evaluation of the same samples.

Adenocarcinoma↗
PubMed1976

Goals of the cytology automation program of the National Cancer Institute.

The immediate goal of the Cytology Automation Program of the National Cancer Institute is the development of an automated system which will screen appropriate specimens from asymptomatic women for squamous cell carcinoma of the cervix and its precursor lesions, dysplasia and carcinoma in situ. This system should make one of three decisions on each specimen: normal, abnormal or inadequate with an acceptably low false negative rate. It is expected that the next step in evaluation of patients whose specimens have been identified as abnormal by the automated system would be a manual evaluation of a traditional cytologic specimen.

Autoanalysis↗
PubMed1968

DNA content of neurons in the cat hippocampus.

The DNA content of individual cell nuclei of cat hippocampus has been measured by means of a scanning cytophotometric technique. The pyramidal cells are tetraploid, whereas the remaining cell types, which include glial cells, interneurons, and the granule cells of the dentate gyrus, are diploid. Nuclei of tetraploid pyramidal cells are significantly larger than those of diploid granule cells.

Animals↗
PubMedDate not supplied

Effects of testicular irradiation on stem cell survival, hormonal environment and spermatogenic cells in Wistar rats.

Adult male Wistar rats were exposed to 3.0 Gy local testicular irradiation. Testes of irradiated and non-irradiated rats were examined histologically and flow cytometrically, at several intervals up to 78 days after irradiation. Concentrations of follicle-stimulating hormone, luteinizing hormone and testosterone determined at these intervals were not different from those of controls. The survival of stem cells were measured 11 weeks after irradiation (with doses varying from 1.0 Gy to 6.0 Gy at 0.6 Gy/min) by means of the repopulation index and by the number of haploid cells (spermatids). Correlation between both methods and relation to stem cell survival were discussed. The dose response curves yielded D0 values of stem cell survival of 2.33 +/- 0.06 Gy (repopulation index) and 2.08 +/- 0.08 Gy (number of haploid cells). The D0 value of the rat was not much different from that found in mice. It was concluded that the used parameters can offer insight when studying hormonal substances during irradiation.

Animals↗