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Biomedical subjects

C J Anderson

Publications and source records attributed to C J Anderson.

114 records · Page 7Linked to original sources
PubMedDate not supplied

An improved measurement of progesterone in saliva and clinical applications.

Measurement of progesterone in saliva offers several advantages when compared to assays of serum progesterone, especially when ovarian activity is being assessed. Most published methods for the determination of progesterone in saliva are based on assays developed in research centers, which employ "in-house" reagents that are critically dependent on supplies of highly selected antisera. In this report, the adaptation of a readily available commercial progesterone "kit", the Pantex Immunodirect Progesterone (125I) is described for the measurement of salivary progesterone. A single extraction step was added, however, to improve assay performance and to ensure that total salivary progesterone was measured.

Female↗
PubMedDate not supplied

Enhancing reciprocity between mother and neonate.

Thirty mothers of newborn infants were assigned to one of three equal-sized treatment groups to assess the effects on maternal responsiveness of an early intervention designed to familiarize mothers with the capabilities and individual characteristics of their infants. Mothers in one treatment group observed the administration of the Brazelton Neonatal Behavior Assessment Scale to their infants, were given an explanation of each item on the scale, and were told their infants' responses. Mothers in a second group were given the same individualized explanations about the scale items and their infants' responses, but did not observe the events. A third group of mothers, which served as control group, received individual instruction on infant furnishings. Maternal responsiveness was enhanced significantly in the "show-and-tell" group but not in the "tell-only" group. Parallel effects on infant responsiveness were also observed. '

Adaptation, Psychological↗
PubMedDate not supplied

Combined topical and subconjunctival anesthesia in cataract surgery.

A combined technique of topical anesthesia (TA) and subconjunctival anesthesia (SCA) was used in 73 consecutive patients undergoing scleral tunnel phacoemulsification cataract surgery. Medical records were evaluated preoperatively, intraoperative, and postoperatively. A patient questionnaire was used to obtain subjective intraoperative and postoperative information. Preoperative and intraoperative sedation of varying degrees was necessary. Ninety-five percent of the patients reported no pain. No patients required additional retrobulbar or peribulbar anesthesia. The most frequent postoperative problems were pain, a need for patching, and headache. The combined TA and SCA technique appeared safe, medically acceptable, and cost-effective.

Administration, Topical↗
PubMedDate not supplied

Development of the anti-ribosomal P autoantibody response.

OBJECTIVE: Autoantibodies to the ribosomal P phosphoproteins (anti-P) appear almost exclusively in SLE. The mechanism by which these autoantibodies appear is unknown. The aim of this study was to determine if IgM and IgG isotype switching occurs during the development of the anti-P autoantibody response. METHODS: Patients who acquired IgG anti-P during clinical observation had their serial serum samples from this period screened for IgM and IgG anti-P by immunoblots and ELISAs. Rabbits were immunized with the immunodominant peptide of the ribosomal P proteins ("P-peptide") cross-linked to bovine serum albumin. Their serial samples were also analyzed for IgM and IgG anti-P before and after repeated immunizations. RESULTS: Two patients were identified who developed transient low titer IgM anti-P that preceded the development of their IgG anti-P. Both patients were clinically well during their peak of IgM anti-P, and developed flares of disease coincident with their high titered IgG anti-P response. The "P-peptide"-immunized rabbits developed an IgM anti-P response that preceded IgG anti-P, and that decreased over time. CONCLUSION: We conclude that IgM to IgG isotype switching occurs in the anti-P autoimmune response. These findings suggest that the autoimmune response to the ribosomal P proteins may be antigen-driven.

Adult↗