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Biomedical subjects

C J Allen

Publications and source records attributed to C J Allen.

45 records · Page 3Linked to original sources
PubMed1982

The use of a protein A conjugate in an indirect enzyme-linked immunosorbent assay (ELISA) of four closely related baculoviruses from Spodoptera species.

The virus particles from four closely related insect nuclear polyhedrosis virus (NPVs) isolated from their homologous Spodoptera spp. hosts were examined using an indirect enzyme-linked immunosorbent assay (ELISA), employing both swine anti-rabbit and protein A conjugate. Results from both systems indicated that S. littoralis NPV is serologically distinct from the other three viruses, S. frugiperda NPV, S. exempta NPV and S. exigua NPV, which are closely related. The relatedness of these viruses is discussed with reference to existing knowledge and the results obtained from both conjugate systems.

Antigens, Viral↗
PubMed1981

Biological and biochemical investigations on five European isolates of Mamestra brassica nuclear polyhedrosis virus.

Five multiply enveloped European isolates of Mamestra brassicae nuclear polyhedrosis virus (Oxford, German, French, Dutch and Danish) were found to be very closely related serologically using the enzyme linked immunosorbent assay (ELISA) double antibody sandwich method and immunodiffusion. By SDS-polyacrylamide gel electrophoresis of viral proteins and restriction endonuclease analysis of DNA using seven enzymes there appeared to be two variants as the Oxford and German isolates were distinct from the other three. The German isolate was shown to be more susceptible to Nonidet P40 detergent treatment affecting some nucleocapsid structural polypeptides which also reduced antigenicity in gel immunodiffusion plates. In bioassays of polyhedra, the Dutch isolate showed a higher LD50 than the other viruses although this was not statistically significant.

Antigens, Viral↗
PubMed1979

A comparative study of atropine methonitrate, salbutamol, and their combination in airways obstruction.

Dose-response relationships of the cholinergic antagonist, atropine methonitrate, and the beta-adrenergic agonist, salbutamol, were examined by cumulative dose techniques. A wet aerosol, 1.5 mg atropine methonitrate produced a maximum response. The response to 200 microgram of salbutamol from a pressurised aerosol was close to maximum. Secondly, the bronchodilator response of salbutamol microgram was compared with atropine methonitrate 2 mg and placebo in 18 asthmatic patients in a randomised crossover study. In 11 of them the bronchodilator response of the combination of salbutamol and atropine methonitrate was evaluated. Atropine methonitrate produced a similar peak bronchodilator effect to salbutamol, but its effect was more prolonged, the response being significantly greater at four and six hours than with salbutamol. The combination of drugs produced a significantly greater and more lasting bronchodilatation than either of the drugs alone. Despite mild side effects, atropine methonitrate, either alone or in combination with an adrenergic drug, appears to have a place in the treatment of sever reversible airway obstruction not adequately controlled by conventional treatment.

Aerosols↗
PubMedDate not supplied

Sputum cell counts and exhaled nitric oxide in patients with gastroesophageal reflux, and cough or asthma.

BACKGROUND: Gastroesophageal reflux (GER) is commonly associated with chronic cough and asthma, but there is little or no information on the nature of any associated airway inflammation. OBJECTIVE: To observe whether the association with GER worsens airway inflammation in patients with chronic cough or asthma. PATIENTS AND METHODS: The airway inflammatory indexes in induced sputum and exhaled air were examined in a cross-sectional study of 11 patients with cough and GER, nine patients with mildly symptomatic asthma and GER, nine patients with mildly symptomatic asthma without GER and nine normal, healthy control subjects. GER was shown objectively by 24 h ambulatory pH recording. RESULTS: The sputum total cell count, the proportion of neutrophils and macrophages, and the fibrinogen level were normal in all four groups, with no significant differences among the groups. The sputum eosinophil and metachromatic cell percentages, and eosinophil cationic protein levels were normal in patients with cough and GER. They were significantly increased in patients with asthma compared with healthy subjects (P<0.01) and patients with cough (P<0.01), but were not different between groups with and without GER. Exhaled nitric oxide levels showed similar results (P<0.01). The correlations between the number of episodes of reflux and the proportion of sputum eosinophils, neutrophils or exhaled nitric oxide were modest but not significant. CONCLUSIONS: GER, when associated with cough or mildly symptomatic asthma, does not cause or aggravate existing airway inflammation as measured by induced sputum cell counts and fibrinogen level, or by exhaled nitric oxide.

Adult↗