In confluent cultures of 3T3 fibroblasts, incubated for 24 h with 1,10-phenanthroline at 10(-5)--10(-9) M, the activity of prolyl hydroxylase was significantly increased. 1,10-Phenanthroline was inhibitory at concentrations greater than 10(-4) M. The stimulatory effect of 1,10-phenanthroline manifests itself after 6 h incubation and increased with time up to 48 h. 2,2'-dipyridyl and 5,6-dimethyl-1,10-phenanthroline were also stimulatory; a nonchelating analog, 1,7-phenanthroline had no effect. Cycloheximide did not modify the 1,10-phenanthroline effect. The stimulatory effect does not seem to depend on the shift of an inactive precursor of prolyl hydroxylase to an active form because 1,10-phenanthroline was shown to be ineffective in logarithmically growing cells. While dialysis of washed and homogenized cells significantly increased prolyl hydroxylase activity in cell extracts, undialyzed 1,10-phenanthroline treated samples exhibited higher prolyl hydroxylase activity than dialyzed controls. These data suggested to us that 1,10-phenanthroline and other chelating agents may be forming complexes with certain metal ions or protein-metal ions which are inhibitory towards prolyl hydroxylase.

2,2'-Dipyridyl↗

PubMed1975

The pregnant psychotherapist in training: some preliminary findings and impressions.

With more women entering general medical and psychiatric training, the more that supervisors and staff can understand the impact of the resident's pregnancy on the resident and others, the more helpful they can be to her and she to herself. The authors' work was done at what was formerly the Woman's Medical College of Pennsylvania, which has had a larger percent of women in psychiatric training than most programs. This report is derived from a pilot study on the pregnant therapist.

Female↗

PubMed1970

Ohio hospitals' quality control and staff utilization program.

Explore the source record for details and available documents.

Hospital Administration↗