Search PubMed⌕ Search

Biomedical subjects

C Henderson

Publications and source records attributed to C Henderson.

At least 109 records · Page 6Linked to original sources
PubMed1987

Role of Chlamydia trachomatis in postpartum endometritis.

To investigate the role of Chlamydia trachomatis in puerperal endometritis, 72 patients with endometritis following vaginal or cesarean section delivery were studied. Blood, urine and endometrial cultures for aerobic and anaerobic bacteria and for C trachomatis were performed for all patients. C trachomatis was isolated in 25% of patients with endometritis. Patients with post-vaginal-delivery endometritis and a positive C trachomatis culture had a later onset of infection as well as a favorable clinical response to treatment despite persistence of C trachomatis in their endometrial cultures at the end of therapy. Cephalosporins failed to eradicate C trachomatis from endometrial cultures after five days of intravenous therapy.

Adult↗
PubMed1985

Sex differences in the dendritic branching of dentate granule cells following differential experience.

Male and female hooded rats were raised from weaning in either a complex or an isolated environment in two separate replications. After one month, the brains were Golgi-Cox stained and dendritic fields of dentate gyrus granule cells were quantified. There was a sex difference in response to the environment. Females raised in the complex environment had more dendrite per neuron than females from the isolated environment in both replications. This difference was evident chiefly in the length of dendritic branches. Males showed few differences in response to the environments in either replication and, to the extent that there were differences, there was a slight tendency for isolated males to have more dendrite per neuron than males from the complex environment. In comparisons between the sexes within an environment, males had more dendritic material per neuron than females in the isolated environment while females had a larger dendritic tree than males in the complex environment. The above pattern of differences was not altered when hemisphere or location of the cell body within the granule cell layer were taken into account, although the shape of the dendritic tree varied with the cell's position in the layer in all groups. Thus, females show greater structural change in the dentate granule cells in response to these environments than do males.

Animals↗
PubMed1984

Differential rearing experience, gender, and radial maze performance.

Since both differential rearing and gender have been known to affect maze abilities, the present study examines the performance of male and female hooded rats raised from weaning in either a complex environment (EC) or isolated environment (IC) on the 17-arm radial maze. In two separate replications, EC rats learned the maze more quickly and accurately than IC rats, as assessed by total errors, the number of correct choices to the first error, and the number correct in the first 17 choices. However, EC rats were more likely than IC rats to employ an adjacent-arm strategy which may have contributed to their superior performance. There were no gender differences or environment by gender interaction effects on any measure of accuracy or adjacent arm strategy in either replication. It appears that the performance of both male and female rats on the 17-arm radial maze is similarly influenced by the rearing environment.

Animals↗
PubMed1983

Levels of circulating and urinary oestrogens during pregnancy in the marmoset monkey (Callithrix jacchus).

The levels of immunoreactive oestrone, oestradiol-17 beta and oestriol in plasma and urine were measured during early, mid- and late pregnancy in the marmoset monkey. In plasma, unconjugated oestrone remained less than 2% of total (conjugated plus unconjugated) oestrone throughout gestation, whereas unconjugated oestradiol-17 beta increased from 3% of the total value in early and mid-pregnancy to 35% in late pregnancy. The reversal in the unconjugated oestrone: oestradiol-17 beta concentration ratio from early (12:1) to late (0 . 15:1) pregnancy occurred despite the continuing predominance of oestrone in terms of total hormone. Total oestriol was measurable but in relatively low concentrations. Oestradiol conjugate was the predominant urinary oestrogen metabolite measured at each stage of pregnancy. The pattern of urinary oestrone and oestradiol-17 beta reflected plasma levels of total hormone, rather than unconjugated hormone, showing no further increase after mid-pregnancy. In contrast, oestriol increased throughout pregnancy and to a proportionately greater extent than oestrone or oestradiol-17 beta, but at lower absolute levels. High-pressure liquid chromatography of urine extract indicated the presence of considerable amounts of oestrogen immunoreactivity not accounted for by oestrone, oestradiol-17 beta and oestriol and with a retention time similar to that of 16 alpha-hydroxyoestrone. Gas chromatography and mass spectroscopy provided further evidence to suggest that 16 alpha-hydroxyoestrone is an abundant urinary oestrogen metabolite during pregnancy in the marmoset monkey.

Animals↗
PubMed1983

Circulating oestrogen concentrations during pregnancy in the African elephant (Loxodonta africana).

Oestrone, oestradiol-17 beta and oestriol were measured in plasma samples from non-pregnant and pregnant African elephants shot in the wild. Enzymic hydrolysis of plasma showed that approximately 90 and 96% of the total (i.e. conjugated plus unconjugated) concentrations of oestrone and oestradiol-17 beta, respectively were represented by conjugated hormones. Unconjugated oestrogens remained low (less than 50 pg ml) in all samples, with no distinction between non-pregnant and pregnant animals. Levels of total oestrone during pregnancy varied between 160 and 594 pg/ml but were not significantly different from non-pregnant values. Total oestradiol-17 beta concentrations were significantly elevated during pregnancy (P less than 0 X 01) and, despite considerable individual variation (193-1428 pg/ml), were consistently higher than non-pregnant values after 6 months of gestation. The elevated levels of oestradiol-17 beta resulted in a reversal of the total oestradiol-17 beta: oestrone concentration ratio at about 6 months of pregnancy. Concentrations of total oestriol did not exceed 103 pg/ml. An indirect method of measurement indicated that oestradiol-17 beta sulphate was probably the most abundant circulating oestrogen during pregnancy in the African elephant.

Animals↗
PubMed1983

Relationship between ovarian and placental steroid production during early pregnancy in the marmoset monkey (Callithrix jacchus).

Concentrations of progesterone, 17 alpha-hydroxyprogesterone, oestrone and oestradiol-17 beta in peripheral and utero-ovarian vein blood were measured during the first 60 days of pregnancy. The same hormones were also measured in peripheral blood samples from non-fertile cycles. Peripheral levels of 17 alpha-hydroxyprogesterone, oestrone and oestradiol increased gradually during early pregnancy whereas concentrations of progesterone declined. The patterns of secretion of progesterone, 17 alpha-hydroxyprogesterone and oestrone, but not oestradiol, were significantly different in fertile and non-fertile cycles by 15 days after ovulation. Comparison of hormone values in peripheral and utero-ovarian vein samples from ovaries with and without corpora lutea (Days 7, 9, 13, 21, 40 and 60 of pregnancy) showed that: (a) progesterone was secreted by the corpus luteum until at least Day 40 by which time there was also placental secretion; (2) although 17 alpha-hydroxyprogesterone was secreted by the corpus luteum, the relative contribution of luteal and placental secretion after Day 21 was not clear; (3) oestrone secretion by the corpus luteum was no longer detectable by Day 40, but placental oestrone secretion appeared to be present by this time; (4) the corpus luteum did not secrete significant amounts of oestradiol at any stage of early pregnancy, although there was evidence for placental secretion by Day 40. These results suggest that progesterone secretion by the corpus luteum of early pregnancy continues beyond the time when oestrogen secretion has declined. The corpus luteum to placental shift in the marmoset appears to occur at a later stage of pregnancy than it does in the macaque monkey and probably also in man.

17-alpha-Hydroxyprogesterone↗
PubMed1982

Formation and identification of cytoskeletal components from liver cytosolic precursors.

Liver cytosol forms a macroscopic fibrillary network in the presence of low concentrations of MgCl2. This process represents the generation of 3- to 11-nm filaments from soluble precursors, involving selectively at least 12 major polypeptides. Similar polypeptides are enriched in the detergent-insoluble fraction from hepatocytes, suggesting that they may be important constituents of the native cytoskeleton. AcA 34 gel-permeation chromatography resolves the cytosol into three independently "polymerizing" peaks: A, B, and C. The formation of filaments follows biphasic kinetics in peaks B and C, whereas peak A lacks the slow phase. Filament formation in all three systems is inhibited by 1-15 mM inorganic phosphate, 10 mM NaF, or 10 mM sodium molybdate. The polymerization of peak C only is inhibited by 0.2-2 mM ATP. CaCl2 (1-100 microM) has no apparent regulatory effect. Two-dimensional polypeptide analysis and peptide mapping show that actin is a major component of peak C, while peaks A and B contain prominent polypeptides that may be related to intermediate filament subunits. In addition, all three systems contain two or three high molecular weight (greater than 170,000) polypeptides that may participate in modulating and extending the filament network. The filaments from peaks A and B are soluble in 8 M urea and reform on removal of the urea in the presence of 5 mM MgCl2. The polypeptide composition remains constant through three such cycles.

Actins↗
PubMed1982

Plasma progesterone levels throughout the ovarian cycle of the common marmoset (Callithrix jacchus).

Radioimmunoassay of progesterone in marmoset plasma has been used to determine ovarian cycle length. Total cycle length was 30.1 +/- 3.8 days (mean +/- SD, n = 30, range 24-41 days, median 29.5 days). The pre-ovulatory (follicular) phase, during which progesterone levels were below 10 ng/ml, lasted for 8.8 +/- 3.7 days (mean +/- SD, n = 30, range 3-20 days, median 8.5 days). The post-ovulatory (luteal) phase, during which progesterone levels were greater than 10 ng/ml, lasted for 21.5 +/- 2.2 days (mean +/- SD, n = 30, range 14-29 days, median 21.5 days). Total cycle length was almost twice that recorded in an earlier study. The reasons for this difference are discussed.

Animals↗
PubMed1982

Role of WHO.

Explore the source record for details and available documents.

Breast Neoplasms↗
PubMed1980

The influence of extracellular hydrogen on the metabolism of Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium.

Strains of three anaerobic rumen bacteria, Bacteroides ruminicola, Anaerovibrio lipolytica and Selenomonas ruminantium, were able to use extracellular H2 to reduce fumarate to succinate. Each bacterium possessed membrane-bound hydrogenase and fumarate reductase activity. Membrane-bound cytochrome b was reducible by H2 and oxidizable by fumarate in each bacterium. The apparent Km values for hydrogen of the hydrogenases were 4 . 5 x 10(-6) M, 1 . 4 x 10(-5) M and 4 . 4 x 10(-5) M for B. ruminicola, A. lipolytica and S. ruminantium, respectively. The apparent Km values for fumarate of the fumarate reductases were approximately 1 . 0 x 10(-4) M for each bacterium.

Anaerobiosis↗
PubMed1978

The role of carbonate in the metabolism of glucose by Butyrivibrio fibrisolvens.

The amount of Na2CO3 added to semi-synthetic medium determined the length of the lag phase, the growth rate and the dry weight of three strains of Butyrivibrio fibrisolvens (WV1, NOR37, B835). With increasing CO3(2-) concentration the molar growth yield of bacteria, from glucosewas increased and, of the fermentation products, formate increased more than the other acids. CO3(2-)-limited cultures of strain WV1 (Group 2 Butyrivibrio) and strain NOR37 (Troup 1 Butyrivibrio) incorporated 14CO3(2-) into lactate and formate. In NOR37, lactate and formate had equal specific activities; in WV1, the formate specific activity was twice that of lactate. Strain WV1 had an active pyruvate synthase and an energy-dependent exchange between CO3(2-) and formate was demonstrated. In strain WV1 butyrate was produced mainly from glucose.

Carbonates↗