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C Hauser

Publications and source records attributed to C Hauser.

150 records · Page 9Linked to original sources
PubMed1984

Spontaneous and pokeweed mitogen-induced in vitro IgG production specific for S. aureus cell wall determinants in man.

A system has been established to produce in vitro IgG specific for cell wall determinants of S. aureus by human peripheral blood mononuclear cells (PBMC). PBMC of healthy individuals, of patients with S. aureus infections and of patients with other bacterial infections were cultured for 12 days. In the culture supernatants (SN) total IgG was determined by a competitive RIA, and IgG to purified cell walls (PCW) of S. aureus strain H by a two step ELISA. PBMC of 11 healthy persons produced anti-PCW IgG upon stimulation by pokeweed mitogen (PWM). This indicates the presence in some healthy persons of circulating B cells which can be induced in vitro to synthesize PCW specific IgG. PBMC of S. aureus infected patients, however, synthesized anti-PCW IgG in culture medium alone. This is the first description of spontaneous in vitro production of specific IgG during a bacterial infection and may be analogous to short phases of spontaneous specific IgG production described after immunizations and viral infections. Finally, compared to healthy individuals, an increased total IgG synthesis in vitro by PBMC obtained from patients with S. aureus and from patients with other severe bacterial infections was found. It is concluded that this polyclonal B cell activation has been initiated in vivo. Its biological significance is unknown.

Adult↗
PubMed1979

[Histopathologic classification of malignant melanoma of the skin. Retrospective study of 149 cases].

In a retrospective study, the histological slides of 149 cutaneous melanomas are classified according to CLARK et al. (1969) into four levels of dermal invasion and three types of melanoma. Simultaneously, the depth measured in millimeters is added in a slightly modified version of BESLOW's method. This demonstrates the practicability of CLARK'S classification with only a few routine sections. The presumptions are (1) The tumor must be cut through its largest extension. (2) The bordering parts of the lesion in the epidermis must be examinable. (3) The specimen must be excised down to the subcutaneous fat. (4) Beside the Hämalaun-Eosin stains, a Van Gieson Elastica stain should be available to determine the level of invasion. Although it is easy to recognize level V, it is difficult to distinguish level II from III or III from IV in some cases. Our material, as compared to the literature, contained more nodular melanomas and lesions of levels IV and V. The reasons for this are discussed. The prognosis of nodular melanomas is worse than that of superficial spreading melanomas even when they are classified into the same level of invasion. A good correlation between the survival rate and the level of invasion is shown. In addition, the prognostic value of the histological staging can probably be optimized by measuring the vertical extent of the lesions in millimeters.

Humans↗
PubMedDate not supplied

Allergen specificity and endothelial transmigration of T cells in allergic contact dermatitis and atopic dermatitis are associated with the cutaneous lymphocyte antigen.

Recent investigations have indicated a role for antigen-specific T lymphocytes in the local skin immunity. The cutaneous lymphocyte antigen (CLA) is supposed to represent a skin-homing receptor for T cells. Inhibition experiments with specific monoclonal antibody demonstrate that CLA participates in selective transendothelial migration of memory/effector T cells in vitro by interaction with E-selectin on endothelial cell layers after activation with proinflammatory cytokines. In addition, the receptor-ligand pairs VLA-4/VCAM-1 and LFA-1/ICAM-1 are involved in this process. Moreover, only CLA+, CD45RO+ (memory/effector) T cells freshly isolated from peripheral blood of patients with allergic contact dermatitis or atopic dermatitis specifically proliferate in response to the respective allergen. CLA-, CD45RO- T cells from these patients do not respond to the allergens. In contrast, memory T cells from asthmatic individuals and patients with both asthma and atopic dermatitis express the allergen specificity in both T cell subsets. Tetanus toxoid, a systemically acting antigen, also induces a proliferative response in both CLA+ and CLA- memory/effector T cell subsets. These results strongly support the selective role of CLA in homing T cells to the cutaneous tissues and therefore playing a role in the local immunity and inflammatory reactions of the skin.

Adult↗
PubMedDate not supplied

[Gleich's syndrome].

BACKGROUND: Episodic angioedema with eosinophilia is a new syndrome associating hypereosinophilia, episodic angioedema and elevation of immunoglobulin M. This syndrome, first described by Gerald Gleich, has a good prognosis and no organ involvement. CASE REPORT: A 39-year old woman developed for 10 years, recurrent episodes of swelling, weight gain of 4 to 6 kg, hypereosinophilia and hyperimmunoglobulinemia M and G. All symptoms disappeared spontaneously within a few days. Numerous investigations (cutaneous, cardiac, pulmonary, immunologic, parasitologic) were negative. The patient was recently hospitalized because of increased recurrence of episodes and major discomfort. We noted hypereosinophilia up to 30. 731 eosinophils/mm(3), elevated lactate dehydrogenase to 902 U/l (N: 204-412), elevated eosinophile cationic protein to 371 microg/l (N<12) and elevated immunoglobulin E to 140 U/l (N<100). Renal, pulmonary and cardiac functions were normal. Bone marrow biopsy showed no abnormality. DISCUSSION: Gleich's syndrome is a benign but often incapacitating disease of unknown etiology. Systemic glucocorticoids may control flare-ups.

Adult↗