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Biomedical subjects

C Harwood

Publications and source records attributed to C Harwood.

5 recordsLinked to original sources
PubMed1998

Role of a p53 polymorphism in the development of human papillomavirus-associated cancer.

The E6 oncoprotein derived from tumour-associated human papillomaviruses (HPVs) binds to and induces the degradation of the cellular tumour-suppressor protein p53. A common polymorphism that occurs in the p53 amino-acid sequence results in the presence of either a proline or an arginine at position 72. The effect of this polymorphism on the susceptibility of p53 to E6-mediated degradation has been investigated and the arginine form of p53 was found to be significantly more susceptible than the proline form. Moreover, allelic analysis of patients with HPV-associated tumours revealed a striking overrepresentation of homozygous arginine-72 p53 compared with the normal population, which indicated that individuals homozygous for arginine 72 are about seven times more susceptible to HPV-associated tumorigenesis than heterozygotes. The arginine-encoding allele therefore represents a significant risk factor in the development of HPV-associated cancers.

Alleles↗
PubMed1994

Papulonecrotic tuberculide and erythema induratum as presenting manifestations of tuberculosis.

We describe a patient with two tuberculides, erythema induratum and papulonecrotic tuberculide. At presentation these were the only manifestations of tuberculosis and they responded dramatically to antituberculous therapy. With the rise in incidence of tuberculosis in Western countries these and other presentations of tuberculosis will be seen with increasing frequency and may provide the only clue to the underlying diagnosis.

Adult↗
PubMed1989

Dystrophin in skeletal muscle. I. Western blot analysis using a monoclonal antibody.

The value of analysing dystrophin on Western blots of skeletal muscle for the differential diagnosis of Xp21 muscular dystrophies is now fairly well established. Here we describe a sensitive system based on monoclonal antibodies to dystrophin. The specificity of the antibodies was established and experiments were undertaken to identify the source of dystrophin-related protein bands which were detected on blots of normal skeletal muscle. These investigations formed a necessary preliminary study to the application of the assay to samples of muscle obtained at biopsy from patients with Duchenne and Becker muscular dystrophy.

Antibodies, Monoclonal↗
PubMed1982

A compact computer printer for quality control spirometric data collection.

A new microcomputer, the Datamite III, was tested with a standardized group of known physiologic spirometric inputs. These test inputs were developed by the National Institute for Occupational Safety and Health, and are recommended by the Association for Advancement of Medical Instruments. They exhibit a wide range of flows and volumes along with initial and terminal hesitation in order to test, under the most severe conditions, all aspects of automated data collection, such as initiation and termination thresholds, sampling rate, back extrapolation logic and data reduction. For all measured spirometric parameters, the accuracy of the Datamite III in computing data from these curves was well within all published requirements. Correlation coefficients of 1.000 were obtained between computed and delivered values for forced expiratory volume at 1s (FEV1), forced vital capacity (FVC), FEV1/FVC, and forced expiratory flow between 25% and 75% of the FVC (FEF25-75). In addition, the Datamite III provides maximum technician support for enhanced quality control through unique program features.

Computers↗