Proteins of the basal and microvillous plasma membranes of the human placental syncytiotrophoblast were compared to elucidate the basis for structural and functional differences in the two membranes. Among the proteins common to both membranes were actin, alkaline phosphatase, albumin, transferrin, immunoglobulin G, proteins of Mr 35,000, 55,000 and 180,000, and five immunochemically detected proteins. Each membrane also contained unique proteins. Major microvillous cytoskeleton proteins of Mr 68,000 80,000 and 105,000 (alpha-actinin) were lacking or absent from basal membrane cytoskeletons which instead contained unique proteins of Mr 14,000, 16,000, 220,000 and 240,000. In addition, immunochemical analyses revealed four glycoprotein antigens unique to microvillous membrane and five unique to basal membrane. Fibronectin was also found to be exclusive to basal membrane. The difference in membrane-associated cytoskeletal proteins correlated with the different organization of actin microfilaments at the two membranes. The protein antigens unique to each of the two membranes provided further evidence for the polarization of membrane proteins and functions in the syncytium.

Cell Membrane↗

PubMedDate not supplied

Cell trafficking and role of adhesion molecules in psoriasis.

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Animals↗

PubMedDate not supplied

Glycine uptake by microvillous and basal plasma membrane vesicles from term human placentae.

Like most amino acids, glycine is present in higher concentrations in the fetus than in the mother. Unlike most amino acids, animal studies suggest fetal concentrations of glycine are minimally in excess of those required for protein synthesis. Abnormal glycine utilization has also been demonstrated in small-for-gestational age human fetuses. The mechanism(s) of glycine uptake in the human placenta are unknown. In other mammalian cells glycine is a substrate for the A, ASC and Gly amino acid transport systems. In this study human placental glycine uptake was characterized using microvillous and basal plasma membrane vesicles each prepared from the same placenta. In both membranes glycine uptake was mediated predominantly by the sodium-dependent A system. Competitive inhibition studies suggest that in microvillous vesicles the small percentage of sodium-dependent glycine uptake not inhibited by methylaminoisobutyric acid (MeAIB) shares a transport system with glycine methyl ester and sarcosine, substrates of the Gly system in other tissues. In addition there are mediated sodium-independent and non-selective transport mechanisms in both plasma membranes. If fetal glycine availability is primarily contingent upon the common and highly regulated A system, glycine must compete with many other substrates potentially resulting in marginal fetal reserves, abnormal utilization and impaired growth.

Cell Membrane↗

PubMedDate not supplied

Riboflavin uptake in microvillous and basal membrane vesicles isolated from full-term human placentas.

Riboflavin uptake was characterized using membrane vesicles isolated from the apical (maternal-facing) and basal (fetal-facing) membranes of the syncytiotrophoblast from full-term human placentas. Equilibrium [3H]riboflavin uptake was insensitive to variations in incubation medium osmolarity in contrast to [3H]alanine uptake into an osmotically sensitive space. Osmotic insensitivity suggested riboflavin binding to a membrane component. The dissociation constant of riboflavin binding was similar in microvillous (Kd = 2 microM) and basal membrane vesicles (Kd = 1 microM). Binding capacity was significantly higher in microvillous membranes (Bmax = 11.9 pmol/mg protein). The relatively high affinity binding to the membrane vesicles may represent a first step in riboflavin transport.

Alanine↗

PubMedDate not supplied

ATP independent calcium transport and binding by basal plasma membrane of human placenta.

The transport of large amounts of Ca2+ by the plasma membranes of human placental syncytiotrophoblast is essential to the mineralization of the growing fetal skeleton. We have investigated transport by the basal (fetal-facing) plasma membrane (BPM). Ca2+ was taken up by purified BPM vesicles in a time-dependent manner and equilibrium attained in approximately 60 min. The apparent equilibrium space was many fold higher than that determined using other substrates (e.g. leucine), suggesting that Ca2+ is concentrated or bound within the vesicles. The more rapid uptake and exit in the presence of A23187 indicates that membrane transport is rate limiting and that Ca2+ is internalized within the membrane space. The initial rate of uptake was approximately by measurement during the first 2 s of incubation. Concentration dependence data were fit to a Michaelis-Menten model with one saturable site and diffusion (Km = 12 microM; Vmax = 4 nmol/min/mg; KD = 39 nmol/min/mg/mM). Saturable Ca2+ binding (Kd = 16 microM; Bmax = 3.4 nmol/mg) was of lower capacity than previously observed for microvillous membrane.

Adenosine Triphosphate↗

PubMedDate not supplied

Transferrin receptors in the basal plasma membrane of the human placental syncytiotrophoblast.

Transferrin was identified in a preparation of human syncytiotrophoblast basal plasma membrane. Such transferrin was shown to be bound to an amphiphilic membrane protein of subunit mol.wt 94 000 by crossed hydrophobic interaction immunoelectrophoresis and immunoprecipitation with antibodies to transferrin. Basal plasma membrane bound 125I-labelled transferrin in a saturable, reversible manner with high affinity (Kd = 2.5 +/- 0.6 X 10(-9) M). The maximum binding capacity (0.9 +/- 0.2 pmol transferrin/mg membrane protein) was approximately a half of that of microvillous membrane. The basal membrane transferrin receptors were similar to microvillous receptors in that their affinity for diferric transferrin was higher than that for apo-transferrin and transferrin dissociation was negligible at pH 5.0 but rapid at pH 7.4. We conclude that syncytiotrophoblast basal plasma membrane possesses a receptor similar, if not identical, to that on the microvillous membrane. These receptors are thus in a position to participate in iron transfer to the fetus or potentially to have alternative functions in the syncytiotrophoblast.

Biological Transport, Active↗

PubMedDate not supplied

Type I insulin-like growth factor receptors in the BeWo choriocarcinoma cell (b30 clone) during cell differentiation.

The expression of insulin-like growth factor (IGF) receptors in the differentiating human trophoblast was studied using the b30 clone of the BeWo choriocarcinoma cell line (BeWob30) as a model system. This clonally derived cell line differentiates over 48-72 h, in culture, to form syncytiotrophoblasts when intracellular cAMP levels are elevated by exposure to 100 microM forskolin (FSK). IGF receptors were studied at various times during the differentiation process by measuring the specific binding of [125I]-IGF-I and [125I]-IGF-II to attached cells. First, [125I]-IGF-I bound to a single class of binding sites in the untreated cells (KD approximately 1-2 x 10-10 M) that exhibited binding specificity characteristic of the type I IGF receptor (IGF-I > or = IGF-II > > Insulin). FSK treatment resulted in a two- to threefold increase in the number of these binding sites. Increased receptor expression was observed as early as 24 h after FSK treatment and remained elevated for at least 72 h. Next, [125I]-IGF-II bound to two classes of binding sites in the untreated cells, a high-affinity (KD approximately 2.5 x 10(-10) M), low-capacity site and a low-affinity (KD approximately 6 x 10(-9) M), high-capacity site. The Bmax and KD of the high-affinity site suggested that it represented the type I IGF receptor. Competition studies revealed that 15-20 per cent of total [125I]-IGF-II binding only was sensitive to IGF-I competition in the untreated cells. After FSK treatment, however, unlabelled IGF-I inhibited 60-70 per cent of specific [125I]-IGF-II binding. Scatchard analysis revealed a two- to fourfold increase in the number of both binding sites with no change in their respective binding affinities. Cross-linking analysis demonstrated that [125I]-IGF-II bound to two structurally distinct binding sites in the untreated BeWob30 cell consistent with both the type I and II IGF receptors. After FSK treatment, however, there was an increase in the relative amount of [125I]-IGF-II associated with the higher affinity type I IGF receptor. The BeWob30 cells expressed no insulin receptors at any stage of differentiation. These data demonstrate that the BeWob30 choriocarcinoma cell line expresses both type I and II IGF receptors. Induction of cell differentiation is associated with an increase in type I IGF receptors expressed at the cell surface. These receptors bind IGF-II with high-affinity, providing additional binding capacity for locally available IGF-II. These data are consistent with specific roles for the type I IGF receptor in regulating differentiated trophoblast cell function. Furthermore, the early rise in type I IGF receptor number suggests they may play a regulatory role in the differentiation process itself.

Binding Sites↗

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Low-vision consultants: an innovative approach.

1. There is a growing population of persons suffering from visual impairments, and their needs must be addressed as their numbers increase. 2. Professionals in the field of ophthalmology have an obligation to meet the challenge of an increasing population of visually impaired persons by offering comprehensive service delivery to their patients. 3. The development of the low-vision consultant has yielded very positive results in the physical, psychological, and social rehabilitation of visually impaired patients.

Consultants↗

PubMedDate not supplied

Endosseous implants for total support and retention of a complete mandibular removable prosthesis.

This is a clinical report illustrating and discussing the use of endosseous root-form implants, both anterior and posterior to the mental foramen, in support of three separate Hader bars which in turn provide total occlusal support, resistance, and retention for a conventionally fabricated full mandibular overdenture. Principles of design, advantages, and disadvantages are listed.

Alveolar Bone Loss↗