Biomedical subjects

C H Shih

Publications and source records attributed to C H Shih.

At least 145 records · Page 8Linked to original sources

PubMed1991

An "attenuator domain" is sandwiched by two distinct transactivation domains in the transcription factor C/EBP.

C/EBP is a rat liver DNA-binding protein which can act as a transcription factor. Its N-terminal portion contains three distinct domains. The first domain (amino acids 1 to 107) appears to be a highly potent transactivator. The second domain (amino acids 107 to 170) does not appear to exhibit either activation or repression activity. This domain is defined as an "attenuator domain" because its presence under four different sequence contexts reproducibly decreases the effect of transactivation of C/EBP. The third domain (amino acids 171 to 245) is a relatively weaker transactivator with a striking proline-rich motif. Deletional analysis of this third domain has shown that a 45-amino-acid region is sufficient for transactivation. This region (amino acids 171 to 215) contains 12 proline, 6 histidine, and mainly hydrophobic or noncharged amino acids. Further mutational analysis of a highly conserved proline-octamer region within this domain indicates that a specific proline content is not crucial for transactivation.

Base Sequence↗

PubMed1991

Interlocking nailing of distal femoral fractures. 28 patients followed for 1-2 years.

We treated 28 fractures of the distal femur with a Grosse-Kempf interlocking nail, and the patients were followed for at least 1 year. Five fractures did not unite, and the rest healed after 5 (3-7) months. Nail breakage was the most serious complication; and the more distal the fracture was, the more easily the nail broke. With the addition of a cast brace and protected weight bearing, a locked nail is a good type of fixation for distal femoral fractures down to 4 cm above the condyles.

Adolescent↗

PubMed1991

Ipsilateral femoral neck and shaft fractures. Retrospective study of 33 cases.

Forty-two consecutive adult patients with a concomitant ipsilateral femoral neck and shaft fracture were treated. The neck fracture was initially missed in 7 patients. Surgery was delayed for a median of 7 days because of the patients' poor general conditions. Thirty-three patients were followed up for 2 (1-6) years. All 33 necks healed, with a union period of 3.3 +/- 1.7 months; and 28 shafts healed after 7.9 +/- 1.8 months. Thus, the femoral shaft determined the total union rate and union period. All the complications, (eight out of 33) occurred in the open procedure group. Our series reveals that ipsilateral femoral neck and shaft fractures have a relatively satisfactory outcome. Closed Küntscher's nailing or interlocking nailing with supplementary Knowles' pinning is the preferable technique.

Adolescent↗

PubMed1991

Effect of long-term alcohol ingestion on the femoral head of rabbit.

Twenty New Zealand rabbits were chosen for this study (5 for controls and 15 for the alcohol group). In the alcohol group, each rabbit was fed with rice wine (2 g/kg/day alcohol) for 1 year. Bone marrow pressure of the femoral basal neck, erythrocyte sedimentation rate (ESR), liver functions, uric acid, triglyceride, cholesterol, amylase, and blood alcohol level were measured at 0 day, 6 months and 1 year. Finally, the rabbits were sacrificed at the end of the study. The livers and femoral heads were examined for pathology. The bone volume and fat cell size of the femoral head were measured with a computerized "Bone Scanner". The results (1 year comparison) were a significantly higher level of cholesterol in the alcohol group (72 +/- 40 mg% vs 26 +/- 8 mg%, p less than 0.05) and greater bone marrow pressure (27.71 +/- 8.97 mmHg vs 18.20 +/- 4.09 mmHg, p less than 0.05), and no significant difference in triglyceride, uric acid and liver function, ESR, etc., when using Student's 2-tailed t-test. Liver changes (fatty liver) occurred in 3 animals in the alcohol group. The effect of alcohol on the trabecular bone volume was not significant (51.5 +/- 4.6 vol.% vs 50.0 +/- 5.2 vol.%), but the average fat cell size of the femoral head was significantly larger than that for normal controls (2,745 +/- 605 mu2 vs 2,185 +/- 458 mu2, p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Alcoholism↗

PubMed1991

Chondrosarcoma of bone: a statistical analysis of prognostic factors.

From 1980 to 1988, 30 cases of chondrosarcoma of the bone were treated surgically. Twenty-seven cases were followed from 6 months to 8.7 years (mean, 3.2 years). In all cases, a clinicopathological review was performed and the significance of prognostic factors were analyzed. The five-year survival of total patients was 88%. The survival was related to adequacy of treatment and histologic grade of the tumor. The five-year survival of adequately treated patients was 100%, but in inadequately treated patients it was 68%. At 8.7 years, 100% of the patients with grade-1 or grade-2 tumors survived, compared to 50% of the six-month survival rate in patients with the grade-3 tumors. None of the grade-1 or grade-2 chondrosarcomas metastasized, while 75% of grade-3 chondrosarcomas metastasized. The incidence of local recurrence was influenced by adequacy of treatment. At the final follow-up, the recurrence rate in the adequately treated group was 7%, while in the inadequately treated group it was 57%.

Adolescent↗

PubMed1991

Treatment of open femoral and tibial shaft fractures preliminary report on external fixation and secondary intramedullary nailing.

From January 1987 to May 1989, a total of 38 adult cases were treated with 15 femoral and 25 tibial shaft shifting procedures from external fixation to reamed intramedullary nailing and followed up for at least 1 year (average, 23 months) at the authors' institution. The indications for the shifting procedures included routine sequential femoral external fixation and failed tibial external fixation treatment such as loss of reduction, atrophic healing process and inability of patients to tolerate casting. The delay period for the shifting procedure was 5 days in the femur and 48 days in the tibia. The union rate for the femur was 93% (14/15), and the tibia, 96% (24/25). The union period after the shifting procedure was 4.8 +/- 1.7 months in the femur, and 5.2 +/- 1.8 months in the tibia. Range of motion of the knee and ankle were satisfactory. Deep infection was the most serious complication, and the tibia was involved more often than the femur (20% to 13%) (p greater than 0.05). The most favorable management of deep infection was local drainage till bony union. We conclude that a shifting operation can be considered as one of the alternative procedures for several femoral or tibial shaft open fractures after fixation with external fixation. Wound and pin tract care, a shorter period of external fixation, a longer delay period before nailing, and perioperative antibiotic use may significantly lessen the infection rate.

Adolescent↗

PubMed1991

Molecular analysis of the p53 alleles in primary hepatocellular carcinomas and cell lines.

We have examined p53 oncogene/anti-oncogene alleles in 10 different human hepatoma cell lines and 18 primary hepatocellular carcinomas. The p53 allele in these hepatoma cell lines appears to be a frequent target of mutation as demonstrated by Southern and Northern blotting, immunoprecipitation and Western blot analysis. In general, the steady state level of p53 specific RNA or protein in these hepatoma cell lines is higher than in normal liver. However, in three out of ten cell lines, normal-sized p53 mRNA cannot be detected. In contrast, the involvement of the p53 allele in primary hepatocellular carcinoma appears to be an exceedingly rare event. Steady state levels of p53 specific RNA in primary hepatomas are practically indistinguishable from those in normal adult liver. Using the polymerase chain reaction technique, we have amplified and subcloned exons 5, 6, 7 and 8 of p53 from 10 different hepatoma samples. DNA sequence analysis of these exon subclones reveals no apparent structural alterations. Finally, synthesis of p53 specific mRNA or protein in a HepG2 human hepatoblastoma cell line does not appear to be affected by gene expression and replication of human hepatitus B virus. Surprisingly, unlike many other kinds of human solid tumors, point mutations in p53 do not appear to be important in primary tumors of hepatocellular carcinomas.

Alleles↗

PubMed1991

Dedifferentiated chondrosarcoma arising from osteochondromatosis. A case report.

Multiple hereditary osteochondromata has a higher incidence (5%-25%) of malignant change into chondrosarcoma than solitary osteochondroma (1%-2%); but only less than 10% of all chondrosarcomas may undergo dedifferentiated change into fibrosarcoma or malignant fibrous histiocytoma. We have a case presented here with osteochondromatosis who was found to have dedifferentiated chondrosarcoma arising from pelvic osteochondroma. Radical extra-compartmental resection was done and followed by chemotherapy and radiotherapy. Recurrence was found 3 months later. Early recognition of malignant change and adequate surgical resection with adjuvant therapy may prevent local recurrence and increase the survival rate.

Adult↗

PubMed1991

Femoral neck fractures. 121 cases treated by Knowles pinning.

The femoral neck fracture remains one of the unsolved fractures. It is a fracture with a high incidence of nonunion and avascular necrosis. One hundred twenty-one mostly young adult patients with femoral neck fractures were treated by Knowles pins internal fixation. Patients were observed for an average of 32 months following surgery. Union occurred in all Garden Stage I and II undisplaced fractures, and the incidence of nonunion was 14% in Garden Stage III and IV displaced fractures. The incidence of avascular necrosis was 5.9% in undisplaced fractures and 34.5% in displaced fractures. There was no significant difference in avascular necrosis rates among age groups. Nonunion and avascular necrosis occurred mainly in displaced fractures. Knowles pinning offers the advantages of few technical failures, early weight bearing, high union rate, and low complication rate.

Adolescent↗

PubMed1990

Post-traumatic fat embolism syndrome--a 10 year retrospective study in Chang Gung Memorial Hospital.

Forty patients with post-traumatic fat embolism syndrome (FES) from January 1977 through December 1986 were retrospectively analyzed. Diagnosis was made according to the criteria modified by Guard. All 40 patients had at least two major criteria, namely change in consciousness and hypoxia. Twenty patients (50%) presented with full-blown clinical features. Prompt respiratory support with oxygen mask or nasal prongs was the first line of treatment. Forty per cent of patients responded well, whereas the others had to be advanced to endotracheal intubation and mechanical ventilation with positive end expiratory pressure. Steroids were given to 80% of patients and better results were achieved than in the group not treated with steroids. The mortality rate was low (2.5%) and only four patients suffered prolonged cerebral sequelae. The clinical course and prognosis cannot be predicted from the severity of the fracture.

Adolescent↗

PubMed1990

Lower extremity compartmental syndrome following snake-bite envenomation--one case report.

Acute compartmental syndrome develops when the intracompartmental pressure rises rapidly, even if only for a short duration. Loss of function and/or viability of the intracompartmental muscles may occur within a short period. Consequently early recognition and management are essential. We report a case where a young child with severe snake bite envenomation who and acute compartmental syndrome who had complete functional recovery following emergent fasciotomy and delayed primary closure.

Compartment Syndromes↗

PubMed1990

Transcriptional activation and repression by cellular DNA-binding protein C/EBP.

A putative transcription factor, C/EBP, isolated from rat liver nuclei, has been shown to bind to at least two different sequence motifs: the CCAAT promoter domain and a core sequence [GTGG(T/A)(T/A)(T/A)G] common to many viral enhancers, including simian virus 40 and human hepatitis B virus. It has been proposed that C/EBP might function as a positive transcription factor by facilitating the communication between promoter and enhancer elements through its dual binding activities to DNA. Surprisingly, results from three different approaches suggest that C/EBP functions as a transcriptional repressor to hepatitis B virus and simian virus 40. Further investigation indicated that C/EBP can function as both a transcriptional activator and a repressor, depending on the reporter gene system.

Base Sequence↗

PubMed1990

Tibial fracture with or without fibular fracture--clinical studies.

This report investigated 145 cases of closed tibial fractures treated with closed reduction and cast immobilization, and followed up at least for 2 years, from 1981 to 1984, at Chang Gung Memorial Hospital of Taiwan. The total delayed or nonunion rate was 28.3% (41 patients), and the healing period was 4.6 months on average (range, 3-6 months). Among them, the delayed or nonunion rate in the tibial fractures with fibular fracture group was 33.3% (35/105), but in the tibial fractures without fibular fracture group it was only 15% (6/40). The difference between the groups was statistically significant (P less than 0.05). The severity of soft tissue injury may be a very important prognostic factor for bone healing.

Adolescent↗

PubMed1990

Ulnar variance and Kienböck's disease. An investigation in Taiwan.

The correlation between negative ulnar variance and the occurrence of Kienböck's disease was evaluated in Taiwan. Two groups of subjects were studied. The first group consisted of 1000 normal subjects and the second of 18 patients with Kienböck's disease. Student's t-test was used to evaluate the significance of the difference between this and other published series. The mean was 0.313 mm in Group 1 and -1.222 mm in Group 2. The percentage with significant negative ulnar variance (the distal ulnar was at least 2 mm shorter than the radius) was 6.0% in Group 1 and 55.6% in Group 2. The difference between the two groups was significant. The mean ulnar variance of normal subjects in Taiwan differed significantly from the variance in Swedes and American blacks but not American whites. In Chinese patients with Kienböck's disease, the ulnar variance was predominantly negative, and the distribution of ulnar variance was similar to that of Swedish or American white patients. This study confirmed the association between negative ulnar variance and the occurrence of Kienböck's disease. This supports Hultén's hypothesis that negative ulnar variance may predispose certain individuals to the occurrence of Kienböck's disease.

Adult↗

PubMed1989

[Adult femoral shaft fracture treated with an intramedullary nail].

Femoral shaft fractures often happen, and usually indicate only one part of the traumatic injuries sustained by the body. Preoperative evaluation and management may be life-saving. The favored treatment of fracture itself is now intramedullary nailing. There were 144 adult cases treated with intramedullary nails and followed up for at least two years at Chang Gung Memorial Hospital from July 1981 to June 1984. The results revealed a high union rate 96.5% (139/144): closed method 100% (34/34), open method 95% (105/110). Significant complications requiring repeated annoying surgical reintervention were rare, only 4% (6/144), due to deep infection or nonunion. Bone union period was about four months. Range of motion of knee was also satisfactory. This study suggests enough benefit of the delayed closed intramedullary nailing in the management of femoral shaft fractures.

Adolescent↗

PubMed1989

Femoral shaft fracture treated with Hoffmann external skeletal fixator in young teens.

External skeletal fixation has been proved to be an effective method in treating complex femoral shaft fracture in adult patients with multiple trauma, extensive soft tissue injury or vascular injury which need reconstructive procedures. This method has the advantages of less extensive surgical exposure, easy wound access, and early ambulation. Twenty-two cases of femoral shaft fracture, between the ages of 10 and 15, were treated with the Hoffmann external skeletal fixator in Chang Gung Memorial Hospital from 1981 to 1985. The average time for union and removal of fixator was 72 +/- 8 days (from 44 to 150 days). The average leg length discrepancy was 0.6 +/- 0.3 cm. There was no deep wound infection, nonunion, rotationary deformity in closed fracture. All patients regained their range of motion of knee within 3 months after removal of the Hoffmann apparatus. The advantages of simple technique, less extensive surgical exposure, early ambulation, easy nursing care, patient comfort and no significant complications make it a good alternative method for treatment of femoral shaft fracture in young teens.

Adolescent↗

PubMed1989

Acute femoral neck fracture in children--preliminary report.

Nineteen children, aged 3-15 years, with acute femoral neck fracture were treated. The follow-up period ranged from 2 years to 6 years, 9 months (avg. 54.8 +/- 14.5 months). The clinical and radiological assessments were carried out according to the assessment system of Ratliff. Undisplaced fractures and all intertrochanteric fractures were treated conservatively and displaced ones by reduction and multiple pinning. The outcomes reveal 14 (79%) with good results, 2 (10.5%), fair and 3 (15.8%), poor. Four hips (21%) developed avascular necrosis with 2 requiring secondary surgical procedures. Both of them also had leg length discrepancy. Another 3 hips (15.8%) developed coxa vara, one of them had mild leg length discrepancy.

Acute Disease↗

PubMed1989

In vitro propagation of human hepatitis B virus in a rat hepatoma cell line.

A rat hepatoma cell line (Q7) of Morris hepatoma origin was transfected with a construct containing the tandem dimer genome of human hepatitis B virus (HBV) and the neomycin-resistant selection marker. The culture medium of several neomycin-resistant single-cell clones was found to accumulate high levels of secreted HBV surface antigen and core-related e antigen. HBV-specific replication intermediates, including relaxed circular and single-stranded DNA with a minus-strand polarity, could be found in both the intracellular fraction and the extracellular culture medium by the Southern blot procedure. One of these clones, designated Q7 HBV-21, was characterized in further detail. DNA polymerase activity was present in the virus particles produced by Q7 HBV-21 cells. Characteristic transcripts of HBV, including the 3.5-, 2.5-, and 2.1-kilobase mRNA as well as a core-gene-related transcript of 2.2 kilobases could be detected. Electron microscopic examination of the conditioned medium from Q7 HBV-21 cells identified 42-nm Dane-like particles as well as 22-nm subviral particles with a spherical or filamentous shape. This Q7 HBV-21 cell line has been maintained in the absence of neomycin for 1 year without losing the properties of HBV DNA replication and Dane-like particle production. Our results strongly suggest that the species barrier of HBV infection is at an early step of viral absorption onto or penetration into the target hepatocytes. This nonhuman system for HBV production in culture could be used to complement the human HepG2 system.

Animals↗