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C H Klaassen

Publications and source records attributed to C H Klaassen.

At least 19 recordsLinked to original sources
PubMed1995

Co-localization and functional coupling of creatine kinase B and gastric H+/K(+)-ATPase on the apical membrane and the tubulovesicular system of parietal cells.

Immunogold labelling of creatine kinase B (BB-CK) and gastric H+/K(+)-ATPase in the parietal cells of the stomach revealed colocalization of these two enzymes on the apical membrane and the membranes of the tubulovesicular system. Upon fractionation of hog parietal cells, a specific fraction of the BB-CK proteins remained associated with the purified vesicles, in which gastric H+/K(+)-ATPase is highly enriched. The BB-CK present in this highly purified preparation was able to support pronounced H+/K(+)-ATPase activity in K(+)-loaded vesicles in the presence of phosphocreatine and ADP, although only low levels of ATP were measured. In contrast, when pyruvate kinase, phosphoenolpyruvate and ADP were used as an ATP-generating system to sustain similar levels of H+/K(+)-ATPase activity, ATP levels were more than 10-fold higher. Changing the experimental conditions such that ATP levels were the same for both systems resulted in significantly elevated H+/K(+)-ATPase activities in the BB-CK/phosphocreatine system in comparison with the pyruvate kinase/phosphoenolpyruvate system. These results indicate that gastric H+/K(+)-ATPase has preferential access to ATP generated by creatine kinase co-localized on the membranes of the vesicles.

Adenosine Diphosphate↗
PubMed1992

Evaluation of an atrophic rhinitis vaccine under controlled conditions.

A vaccine containing inactivated cultures of Bordetella bronchiseptica, toxigenic Pasteurella multocida type D and dermonecrotic P multocida type D toxoid in an oil-in-water adjuvant was given to seven sows, with seven others acting as controls. Half the piglets in each litter were exposed intranasally when four days old to B bronchiseptica and when eight days old to toxigenic P multocida type D. There was considerably less sneezing in the litters of the vaccinated sows and when the piglets were 10 weeks old, only 18 per cent had deformed snouts compared with 74 per cent in the litters of the control sows. The average liveweight gain of the piglets born to vaccinated sows was significantly better (P less than 0.05) between two and 10 weeks of age than that of the piglets born to unvaccinated sows, although there were no significant lower respiratory tract lesions in either group. The conchal atrophy scores were significantly lower (P less than 0.001) in the piglets from the vaccinated sows and were negatively correlated (r = -0.37) with increasing liveweight gain. In the liters of the vaccinated sows, P multocida was not isolated from the nasal passages of the in-contact piglets and from only 7 per cent of those deliberately exposed compared with 65 per cent and 79 per cent, respectively, in the litters of the control sows. P multocida was isolated post mortem from the tonsils of 23 per cent of the piglets of vaccinated sows and from 87 per cent of those from unvaccinated sows.

Animals↗
PubMed1991

D-Dimer latex assay as screening method in suspected deep venous thrombosis of the leg. A clinical study and review of the literature.

We examined the usefulness of a new commercially available latex agglutination test, measuring cross-linked fibrin degradation products (D-Dimer) in patients suspected clinically of having deep venous thrombosis of the leg. In 33 patients both contrast venography and D-Dimer latex assay were performed. In this group the test had a sensitivity of 48% for the diagnosis and a specificity of 100%. With a prevalence of 64% in our group the negative predictive value was 52%. We conclude that the D-Dimer latex assay is not useful in the initial evaluation of patients with suspected deep venous thrombosis of the leg. A review of the literature is given.

Cross-Linking Reagents↗
PubMed1988

[The prophylactic effect of albendazole containing a pulse-release bolus on gastrointestinal helminth infection in calves].

The prophylactic effect of an albendazole containing 'pulse release' bolus on gastrointestinal helminth infection in calves during their first grazing season was tested on a farm in the Netherlands in 1986. Thirty calves were divided into two groups of young calves and two of old calves. On being turned out to grass, calves of one of these groups were given a bolus containing 3 x 2 grammes of albendazole, to be released after thirty-one, sixty-two and ninety-three days. The treated young calves showed a much lower egg-output, produced a lower infestation of herbage, displayed significantly lower pepsinogen levels and showed a significantly better gain in weight compared with the untreated young calves. Differences were not observed between the two groups of older calves, probably because of the fact that one of the treated calves had lost the bolus at an earlier stage.

Albendazole↗
PubMed1979

Glipizide ('Glibenese') in maturity-onset diabetes mellitus.

A clinical evaluation was carried out in 19 maturity-onset diabetics to assess the effectiveness of glipizide in the treatment of newly diagnosed patients (11) not responding to diet alone and of patients (8) who had been inadequately controlled on other oral antidiabetic agents. Patients were studied over a period of 6 months. All of the 11 newly diagnosed patients were adequately controlled with glipizide in a dose of 5 to 15 mg/day (mean 9.6 +/- 3.7 mg/day), as were 5 of the 8 previously treated patients with a dose of 10 to 25 mg/day (mean 18 +/- 6.7 mg/day). None of the patients became hypoglycaemic nor did the blood sugar levels indicate hypoglycaemia in the fasting state. Serum triglyceride levels did not change significantly during glipizide therapy, neither did the patients' weight. No side-effects were reported.

Aged↗