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Biomedical subjects

C Gutierrez

Publications and source records attributed to C Gutierrez.

At least 181 records · Page 10Linked to original sources
PubMed1985

Nasal fractures in children: influence on the development of the nose.

Fifty-seven patients between the ages of zero and 16 years, treated for nasal fractures during childhood by means of closed reduction, have been subjected to follow-up examination after development of the nose has ceased. These patients have been compared to a control group of 50 persons without any known nasal trauma. No difference was found between the two groups with regard to functional complaints, but deviations of the osseous and cartilaginous pyramid, humps and saddle formations, as well as spine formations and deviations of the septum, were particularly prevalent in the fracture group. Crista formation and angulation were found to be equally frequent in both groups. The findings are discussed.

Adult↗
PubMed1985

The importance of nasal fractures during different growth periods of the nose.

Fifty-seven patients who had sustained nasal fractures during childhood were divided into three groups, according to the age at which the fracture had taken place. These three groups were based on the normal periods of development of the nose. They were compared with regard to possible sequelae after fracture. The results are discussed and an example from each group is shown.

Adult↗
PubMed1985

Essential and nonessential sequences in malPp, a positively controlled promoter in Escherichia coli.

A plasmid bearing the malPp promoter was digested with Bal31 to obtain a set of deletions with closely spaced endpoints in the upstream region of this promoter. Some of these deletions were sequenced, and their effect on malPQ expression was determined after having transferred them onto the chromosome. We found that a site which binds the cyclic AMP receptor protein in vitro and which is centered at position -93 with respect to the site of transcription initiation could be deleted without affecting malPQ expression. In contrast, the activity of the malPp promoter decreased abruptly when the deletions reached position -72. The downstream region of the promoter was analyzed by using a technique of "sequence replacement" which involved the selection of Mal+ pseudorevertants from strains which carried small deletions in the -25 region. The pseudorevertants, which expressed the malPQ operon in a manner indistinguishable from wild type, had grossly different sequences downstream from position -38, except for a few positions, some of which must be important for promoter function. By combining all presently available information, it is suggested that the malPp promoter contains three binding sites for its activator, the product of gene malT. These sites are defined by three quasi-identical hexanucleotides present in one orientation around position -37 and twice in the other orientation around positions -60 and -73.

Base Sequence↗
PubMed1985

Chromosomal translocation 1/29 in Santa Gertrudis sires.

Chromosomal analysis of 38 Holstein, 10 Santa Gertrudis, and 5 Zebu sires revealed for the first time the occurrence of the Robertson translocation in this breed. The importance is stressed of cytogenetic studies for sires.

Animals↗
PubMed1984

Point mutations that reduce the expression of malPQ, a positively controlled operon of Escherichia coli.

malPQ is one of three operons controlled by the positive regulator gene malT. With the objective of defining DNA sequences essential for malPQ transcription, we looked for cis-dominant mutations that reduced the level of expression of this operon. We first constructed malP-lac fusion strains, selected from one of them a series of mutants resistant to p-nitrophenyl-beta-D-thiogalactopyranoside (a bacteriostatic compound that enters the cells via lac permease), and retained the clones that contained a mutation reducing the expression of the hybrid operon in a cis-dominant fashion. Nineteen such mutations were sequenced, and their effect on an otherwise wild type malPQ operon was studied. Three of them mapped in a transcribed portion of the operon, and are believed to exert their effect at the translation level. The others map upstream from the transcription startpoint (co-ordinate +1) and help define three DNA segments that must play a predominant role in transcription initiation: the Pribnow box (from positions -7 to -12); and two inverted repeats, extending from position -32 to -36, and -59 to -63, respectively, which are proposed to constitute part of the binding site for MalT protein.

Base Sequence↗
PubMed1984

Intranuclear inclusions in hypothalamic neurons: a study in the ageing hamster.

Intranuclear inclusions have been observed in a wide variety of locations throughout the nervous system. In the present work we have studied the inclusions that appear within the nucleus of non-secretory neurons from both paraventricular and supraoptic nuclei of the hypothalamus of the ageing hamster. We described four morphological types, namely, micro-filamentous, crystalloid, circular and capsuled inclusions. Their relationships with both the nuclear membrane and the nucleolus are discussed in the light of their ultrastructural features.

Aging↗
PubMed1983

Analysis of baseline and BrdU-dependent SCEs at different BrdU concentrations.

In the present paper we have used a rationale based on the development of theoretical equations that define sister-chromatid exchange (SCE) frequencies as a function of two variables, namely the baseline (BrdU-independent) and the BrdU-dependent SCE frequencies. The experimental design includes the estimation of SCE frequencies in second division chromosomes when both cycles occurred in the presence of BrdU and when BrdU incubation took place only during the first cycle in a wide range of BrdU concentrations. The final SCE yields in second division chromosomes could be separated into three different components: (1) The BrdU-independent, 'spontaneous' or baseline SCEs, whose low but biologically significant frequency was calculated to be about 0.06 SCEs per pg of DNA; this figure could be similar for most of the cell types; (2) the BrdU-dependent SCEs whose frequency increases with BrdU dose, probably as a result of BrdU substitution for thymidine; (3) the BrdU-dependent SCEs as a consequence of other cellular factors such as disturbance of nucleotide pool sizes. At high BrdU concentrations (300 microM upward) the three components appear to have a significant value in the final SCE yield, whereas at lower BrdU doses the third component seems to be negligible.

Bromodeoxyuridine↗
PubMed1983

The nasal passage following rhinoplastic surgery.

A follow-up examination has been carried out of 238 patients suffering from nasal obstruction and mouth-breathing. Only 75 per cent of the patients achieved normal nasal passages. The results were significantly poorer in patients suffering from allergic or vasomotor rhinitis, in cases of long-standing mouth-breathing, and in those cases in which the patient had been subjected to earlier rhinoplastic surgery. Our investigation has indicated that a re-evaluation of the indications for surgery in the above-mentioned cases is necessary, and that a more cautious attitude should be taken regarding the possibility of achieving a normal nasal passage following rhinoplastic surgery.

Adolescent↗
PubMed1983

Comparison of radionuclide images and radiographs for skeletal metastases from renal cell carcinoma.

68 metastatic bone lesions proven by biopsy, follow-up radionuclide bone imaging, or radiographs in 18 patients with surgically confirmed renal cell carcinoma were retrospectively analyzed. Bone imaging demonstrated 62 lesions (91%): 48 definite, 7 questionable 'hot' lesions, and, 2 definite, 5 questionable 'cold' lesions. The spine was the most common site. 15 patients had more than one metastasis. 27 lesions were undiscovered by radiographs. Serum alkaline phosphatase was elevated in 12 of 18 patients. It is concluded that radionuclide bone imaging is more sensitive than radiographic examination for bony metastasis from renal cell carcinoma, and that it is important to recognize 'cold' metastatic lesions on the bone imaging in patients with renal cell carcinoma.

Adenocarcinoma↗
PubMed1983

Diffuse abdominal uptake of Ga-67 citrate in a patient with hypoproteinemia.

A 3-wk-old male, with abdominal distention and severe hypoproteinemia from poor nutrition, underwent a study that showed a persistent diffuse abdominal uptake of Ga-67 citrate, indicating pyogenic or tuberculous peritonitis. However, there were no corresponding clinical or laboratory findings. After a 1-wk course of hyperalimentation with albumin, furosemide, and protein, repeat radiographs showed reduction in bowel gas. It is suggested that hypoproteinemia should be considered as a possibility in the differential diagnosis when there is diffuse abdominal uptake of Ga-67 citrate, with careful clinical correlation. Possible mechanism of Ga-67 uptake in the peritoneal cavity is suggested.

Diagnosis, Differential↗
PubMed1982

Columella implants. Reconstruction of the anterior septum.

A follow-up study was conducted of implants used to replace the anterior septal cartilage. The implants used were (1) the cartilaginous septum after correction, (2) parts of the bony septum (when it was impossible to use the cartilaginous septum), (3) or bank cartilage (when there was insufficient autograft material). One hundred cases were examined 12 months after surgery and divided into three groups according to the material used. There was no statistical difference between the percentages of satisfactory results in the three groups: 66% satisfactory and 33% unsatisfactory. Further analysis of the unsatisfactory results indicated that the most difficult feature is judging the size of the implant: too small an implant results in instability, reduction in the nasolabial angle, and retraction of the columella. Previous surgery increases the risk of poor results.

Cartilage↗
PubMed1981

Obtaining a gentle contour to the columella by modifying the maxillary spine.

There are several structures involved in columellaplasty, and all affect the support and shape of the tip, the columella, and the upper lip. The present paper considers only the problem of the deformities of the maxillary spine and its lower extension over the midline, causing broadening and asymmetrical development of the columella as well as a short upper lip, and an obtuse columella-lip angle. During rhinoplasty, the nasal spine can be exposed and reshaped by carving and remodeling with drilling. At the same time, the support this structure provides for the columella and tip can be retained. This paper is based on follow-up of 50 patients on whom surgical correction of the maxillary spine was performed with satisfactory results in 90 percent.

Female↗
PubMed1980

[Removal of foreign bodies from the upper airways and the bronchial tree of small children].

Food remnants are the foreign bodies most frequently aspirated by young children. Larger foreign objects are nearly always rejected spontaneously. In many cases, however, aspiration of foreign bodies lead to death by suffocation. From March 1975 to August 1979, 27 endoscopies removing 22 foreign bodies have been carried out by us. All children were less than 7 years old. The foreign bodies most commonly found were peanuts. Endoscopy was not only carried out when diagnosis was clear, but also if there was suspicion of foreign body aspiration. It is nowadays a treatment without risk when the following conditions are met: 1. Careful medical preparation leading to bronchial dilatation. 2. Effective collaboration with a paediatric anaesthetist. 3. Exact knowledge of the bronchial tree of children.

Bronchi↗
PubMed1979

Purification of human T and B cells by a discontinuous density gradient of percoll.

A simple technique for isolating human peripheral B and T cells is described. This method is based on the different mobility of cells in a discontinuous density gradient of Percoll. A population with a mean of 72.8% of surface stable Ig (B cells) is found in the top layer (fraction I) whereas T cells with less than 3% of Ig are distributed in the two lower layers (fractions II and III). B cells obtained by this method were highly viable and reacted strongly with anti-DRw specific sera, whereas T cells did not respond. The clear-cut difference between positive and negative reactions makes this technique very suitable for DRw typing. The proliferative response to PHA is decreased in fractions I and III while greatly increased in fraction II. The different mitogenic responses in the two fractions containing T cells probably represent two functionally distinct T cell subsets. Cells from all fractions are less activated than unseparated cells when Con A and PWM are used as mitogen. The technique is very rapid, avoids the interaction of cells with their specific receptors and gives both T and B cells in one step, all advantages over other methods commonly used.

Antilymphocyte Serum↗