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Biomedical subjects

C Guo

Publications and source records attributed to C Guo.

At least 127 records · Page 7Linked to original sources
PubMed1998

[Determination of adenosine and 3'-deoxyadenosine in Cordyceps militaris (L.) Link. by HPLC].

The establishment of a quantitative determination method by HPLC for adenosine and 3'-deoxyadenosine in Cordyceps militaris is reported. The sample was extracted with water under ultrasonic management for 2 hours. Chromatographic conditions: YWG C18 10 microns 5 mm x 200 mm; mobile phase consisted of NaH2PO4-NaOH buffer solution (pH 6.8); UV detection at 260 nm. The recoveries of adenosine and 3'-deoxyadenosine were 95.8% +/- 0.64% and 100.6% +/- 0.65% (n = 5) respectively.

Adenosine↗
PubMed1998

[A preliminary study on the relationship between nutritional status and postoperative complications in patients with head and neck malignancies].

OBJECTIVES: To study the role of nutritional factor in the development of postoperative complications in patients with head and neck malignancies. METHODS: Postoperative complications of 96 patients with head and neck malignancies who were operated on were surveyed. Twenty-seven developed postoperative complications; the remaining 69 went uneventful. Comparisons of nutritional status and clinical variables between these two groups were made. RESULTS: 1. The prevalence of poor preoperative nutritional status in the complication group (56%) was higher as compared with that in the noncomplication group (20%) (P < 0.001); 2. values of body weight, triceps skin fold thickness, arm circumference, arm muscle circumference, and creatinine-height index in the complication group decreased much more significantly than in the noncomplication group (P < 0.001); 3. the nitrogen and calorie intake within the first postoperative week was lower in the complication group than in the non-complication group (P < 0.001). CONCLUSIONS: Nutritional factor plays a very important role in the development of postoperative complication and the perioperative nutritional support for head and neck surgical patients should not be ignored.

Adult↗
PubMed1998

[Genetic analysis of varicose vein of lower extremities].

OBJECTIVE: To make a hereditary analysis of varicose vein of lower extremities(VVLE), including hereditary pattern, sex ratio and the transmitted expression male and female of varicose vein of lower extremities. METHODS: The ascertainment probability was estimated according to Weinberg's proband method. The value of segregation ratio P and proportion of sporadic cases X were estimated by the maximum likelihood score method. The calculations were performed using computer program of segregation analysis. The VVLE cases were random samples from inpatients and outpatients of the authors' hospital from 1991 to 1996. RESULTS: There were 68 pedigrees including 80 nuclear families in 3 different mating types:UAU, UAA and AAA. UAU, UAA and AAA were analysed together. UAA and UAU (multiplex families) were analysed respectively. All the values were compatible with autosomal dominant inheritance of incomplete penetrance; the penetrance frequencies were 91.94%, 78.14% and 69. 92% respectively. The proportion of X was 24.32%. The UAU was compatible with autosomal recessive inheritance; the proportion of sporadic cases was 36.75%. CONCLUSION: VVLE is compatible with autosomal dominant and recessive inheritance. The sex ratio of male:female is 2.39:1. The transmission is possible from male and female to male and female both.

Female↗
PubMed1997

A new nutritional assessment in patients with oral and maxillofacial malignancies.

Combined subjective and objective nutritional assessment was performed on admission in 127 patients with oral and maxillofacial malignancies. On the basis of the nutritional assessment result, three typical nutritional parameters-body weight (BW) (X1), mid-upper-arm circumference (MAC) (X2), and hand grip strength (HGS) (X3) were used to establish a new nutritional assessment method that was developed by a computer-based discriminant analysis. The established model was as follows: Y1 = -126 + 1.09X1 + 1.34X2 + 0.23X3; Y2 = -95.63 + 0.96X1 + 1.17X2 + 0.19X3. In the model Y1 was regarded as good nutrition and Y2 as malnutrition. The larger value of Y stood for the patient's nutritional status (i.e. Y1 > Y2, well nourished; Y1 < Y2 malnourished). The new nutritional assessment correlates well with the combined subjective and objective nutritional assessment with a total agreement rate of 88.2%. With its simplicity and accuracy, the new nutritional assessment deserves a wide application in clinical situation.

Adult↗
PubMed1997

Responses of plasma fibronectin to the changes of dietary protein levels in rats.

In this study, the plasma fibronectin was evaluated as a nutritional marker in protein- and protein-energy malnourished rats in comparison with plasma prealbumin, albumin, and total protein. The plasma fibronectin was determined by nephelometric assay based on the interaction of an antigen and antibody. The rats were subjected to three diets, containing 20, 5, and 0% casein, respectively, for 5 wk except in the last 2 wk, the rats on the 0% casein diet were re-fed on a 20% casein diet. The results showed that in the rats on a 5% casein diet (protein malnourished), the plasma fibronectin concentration rose significantly in the early stage and normalized thereafter. In the rats subjected to a 0% casein diet (protein-energy malnourished), the plasma fibronectin concentration did not decline quickly in response to protein depletion, compared with the plasma prealbumin, albumin, and total protein concentrations. Following the re-feeding of a 20% casein diet in the last 2 wk, the plasma fibronectin concentration was restored earlier than other plasma protein concentrations. No significant change in plasma fibronectin concentration was found in the rats receiving a 20% casein diet (control) over the 5 wk feeding period. It is concluded that plasma fibronectin may not be a suitable marker for protein or protein-energy malnutrition, though it is a sensitive index for nutritional rehabilitation.

Animals↗
PubMed1997

Factors influencing survival rate in adenoid cystic carcinoma of the salivary glands.

Ninety-one cases of adenoid cystic carcinoma (ACC) of the salivary glands with more than ten years' follow up were studied to investigate factors influencing the survival rate of patients, which vary according to site, histological type, clinical stage and nature of therapy. The data were statistically analysed for survival curves. Log rank tests were employed to assess the statistical significance of various groups. As a result, it may be concluded that tumour site, clinical stage and histological type are the important factors influencing the prognosis. ACC of the palate and parotid, early clinical stage, glandular/tubular histological type, and tumour without nerve involvement had the best prognosis. ACC in the submandibular gland, maxillary antrum and tongue, advanced clinical stage (stage III and IV), solid histological type, and tumour with nerve involvement had a poor prognosis.

Adult↗
PubMed1997

[Epidemic trend of tuberculous meningitis in children of Heilongjiang province].

OBJECTIVE: To investigate the epidemic trend of tuberculous meningitis in children of Heilongjiang province in recent ten years, and to provide scientific evidence for control of the disease. METHODS: The data, registered by 82 counties' antituberculosis institute, of tuberculous meningitis patients under 14 years were analyzed, and the epidemic trend of the disease was also discussed. RESULTS: The incidence of tuberculous meningitis was 9.58 per 10,0000 populations in 1982, 2.17 per 10,0000 populations in 1992. The rate of annual decrease was 13.8%. CONCLUSION: Participation of the government, improvement of pelple's life standard, control of sources of infection, and vaccination of BCG are effective measures for decreasing the incidence of this disease.

Adolescent↗
PubMed1997

[Production of anti-enteric adenovirus monoclonal antibody by electrofusion and semi-solid medium culture].

Ten million splenocytes from the enteric adenovirus (Ad40/41) immunized mice were fused with 3 x 10(6) myeloma cells by electrofusion. Then, the cells were selectively cultured in methyl cellulose semi-solid medium. Eighteen clones of hybridoma were directely picked out from the culture plates and 7 of them could secret high level specific antibodies to enteric adenovirus. So far, the anti-Ad40/41 virus monoclonal antibodies were successfully generated by the protocal of electrofusion accompanied with selective culture in methyl cellulose semi-solid medium.

Adenoviridae↗
PubMed1997

[Cellular immunity in childhood iron deficiency anemia with recurrent respiratory infections].

OBJECTIVE: To examine interleukin-2 (IL-2) activity,serum soluble IL-2 receptor (sIL-2R) level and T lymphocyte subsets in peripheral blood from 63 childhood iron deficiency anemia (IDA) patients with recurrent respiratory infections(RRI). METHODS: IL-2 activity, sIL-2R level and T lymphocyte subsets were assayed by MTT, ELISA and APAAP, respectively. RESULTS: IL-2 activity, percentages of CD3+ and CD4+ cells as well as the ratio of CD4+/ CD8+ cells in the patients were significantly lower ( P < 0.01),while sIL-2R levels were higher than that in normal controls (P < 0.01). No significant change was found in the percentage of CD8+ cells. CONCLUSION: Cellular immunity was impaired in childhood IDA with RRI.

Anemia, Iron-Deficiency↗
PubMed1996

The 12;21 translocation involving TEL and deletion of the other TEL allele: two frequently associated alterations found in childhood acute lymphoblastic leukemia.

A recurrent t(12;21)(p13;q22) has recently been described in human acute lymphoblastic leukemias (ALLs). This translocation fuses TEL and AML1, two genes previously cloned from translocation breakpoints in myeloid leukemias. In addition, allelic loss of the TEL gene can be detected in 15% to 22% of childhood ALLs. In the present study, we have sought allelic deletions of TEL and the presence of the t(12;21) in 50 children with B-lineage ALL, using a combination of microsatellite typing, fluorescent in situ hybridization (FISH), and analysis of the fusion transcripts resulting from the TEL-AML1 gene fusion. Our results indicate that the association between the t(12;21) and the deletion of the nontranslocated allele of TEL is among the most frequent abnormalities observed in B-lineage ALLs. FISH analysis using several cosmid probes showed that, in one patient with a t(12;21) translocation involving TEL, the second allele had an intragenic deletion. This observation points to TEL as the actual target of 12p12-13 deletions in patients that associate a t(12;21) with a deletion. The TEL-AML1 fusion RNA was found in all patients with the t(12;21) whereas the reciprocal AML1-TEL transcript was only found in a subset of patients, suggesting that only the protein product encoded by TEL-AML1 is likely to play a role in leukemogenesis. The observation that, in two patients with the t(12;21), a deletion of TEL was only present in a subclone indicates that this deletion was a secondary event that occurred after the translocation. The frequent occurrence of TEL deletions in patients with t(12;21) suggests that the deletion of the normal TEL allele subsequent to the t(12;21) provides a further proliferative advantage to leukemic cells.

Adolescent↗
PubMed1996

Kinship and familiarity as factors affecting social transfer of food preferences in adult Mongolian gerbils (Meriones unguiculatus).

Experiments were carried out with Mongolian gerbils (Meriones unguiculatus) to assess whether a socially mediated acquisition of diet selection exists in this species. Results showed that a gerbil was influenced in its diet choices by information extracted during a brief period of interaction with a familiar conspecific that had recently eaten a novel food. Data revealed that the acquisition of a food preference from a conspecific depends on the existence of a social bond between the interacting gerbils. Either genetic relatedness (being brother or sister raised in different litters) or familiarity (being bred in the same litter or being member of a reproductive pair) is necessary for the transfer of information. Unfamiliar and unrelated observer gerbils did not selectively choose their demonstrator's food.

Age Factors↗
PubMed1996

Genomic organization of TEL: the human ETS-variant gene 6.

We have constructed a detailed map of the genomic region containing the ETS-variant gene 6 (ETV6), involved in translocations and deletions associated with hematologic malignancies. Thirty-eight cosmids were characterized belonging to two contigs spanning 340 kb, and an EcoRl restriction map was developed. The gap between the two contigs, 2 kb in size, was closed by PCR. The contigs contain the complete coding sequence and the 5' and 3' UTRs of ETV6. Eight exons accounting for the ETV6 cDNA sequence were identified. The helix-loop-helix (HLH) motif is coded by exons 3 and 4, whereas exons 6-8 code for the ETS DNA-binding domain. All introns show consensus 5' donor and 3' acceptor splice sites. Introns 1 and 2 span 100 and 82 kb, respectively, and introns 3-7 range from 15 to 1.3 kb. An alternative exon 1 (exon 1B) is localized in intron 2. The 5' end of the ETV6 gene is associated with a CpG island characterized by the presence of four Notl, four Sacll, and three BssHll recognition sites and several SP1- and AP2-binding motifs. Alternative polyadenylation at the 3' end of the ETV6 gene generates the three transcripts of 6200, 4300, and 2400 nucleotides, respectively. The ETV6 gene spans 240 kb and is flanked at its 5' and 3' end by D12S1697 and D12S98, respectively. The markers D12S1095 and D12S89 are located in the first intron. Two new DNA polymorphisms were identified in the ETV6 gene, which will be useful for the analysis of loss of heterozygosity reported for the ETV6 gene in leukemia.

Base Sequence↗
PubMed1996

Clinical observation on efficacy of electro-acupuncture therapy in hyperplasia of mammary glands and its effect on immunological function.

Based on our work on electro-acupuncture therapy of hyperplasia of mammary glands and in order to observe further the relationship between immunological function and E2 in hyperplasia of mammary glands, we determined the change not only in immunological function before and after electro-acupuncture in 44 in-patients with hyperplasia of mammary glands and having the same menstruation date, but also in electro-acupuncture treated rat hyperplasia of mammary glands model. We found that there was decrease of immunological function in hyperplasia of mammary glands and this function could be remarkably enhanced by electro-acupuncture both in hyperplasia of mammary glands patients and in rat hyperplasia of mammary glands model. The mechanism implies it might be that the inhibitory effect on immunological function exerted by high concentration of E2 was lowered by electro-acupuncture, hence the immunological function was reinforced. The results of this study are important in exploration of both the pathogenesis of this disease and the mechanism of electro-acupuncture, and in reducing the incidence of breast carcinoma.

Adult↗