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Biomedical subjects

C Godin

Publications and source records attributed to C Godin.

At least 55 records · Page 3Linked to original sources
PubMed1991

Bradykinin stimulates DNA synthesis in competent Balb/c 3T3 cells and enhances inositol phosphate formation induced by platelet-derived growth factor.

Both platelet-derived growth factor (PDGF) and bradykinin were found to induce a growth response in Balb/c 3T3 cells. However, whereas PDGF brought about a five-fold increase in the incorporation of [3H]thymidine into DNA, the response to bradykinin was never more than 50%. When bradykinin was present simultaneously with sub-optimal concentrations of PDGF the response was about 15% greater than with PDGF alone. In contrast, if the cells were made competent by a 5 hr preincubation with PDGF which was then washed away, subsequent addition of bradykinin induced a more than two-fold increase in incorporation of [3H]thymidine into DNA compared with competent cells subsequently incubated with serum-free medium alone. Bradykinin also acted synergistically with insulin when the two agents were added simultaneously to competent cells. PDGF induced marked increases in the concentration of inositol phosphates at 30 min after stimulation, but by this time point any effect of bradykinin had disappeared. However, the simultaneous presence of PDGF and bradykinin induced increases at 30 min that were 50-100% greater than with PDGF alone. It is concluded that the pathways by which PDGF and bradykinin initiate a growth response in BALB/c 3T3 cells only partly overlap. Their actions on the synthesis of inositol phosphates exhibit distinctive temporal characteristics, but can be co-operative at 30 min and at earlier time intervals. This effect was found to be time-dependent, and developed over the first 5 min.

Animals↗
PubMed1990

Hip fractures and Parkinson's disease. A clinical review of 94 fractures treated surgically.

A series of 87 parkinsonian patients with 94 hip fractures were reviewed to reexamine the evidence for internal fixation versus hemiarthroplasty. Forty-seven subcapital Garden Type I and Type II fractures and transcervical fractures were treated by nailing, and 47 Garden Type III and Type IV fractures were treated by hemiarthroplasty. There were twice as many complications in the hemiarthroplasty group as in patients treated by nailing, including four wound infections and five dislocations, all with uniformly bad results. Patients treated by nailing regained greater ambulatory independence than those with a hemiarthroplasty. The parkinsonian patients with hip fractures treated by internal fixation had better results than those treated by hemiarthroplasty; hemiarthroplasty may be even contraindicated.

Aged↗
PubMed1988

[Compartment syndrome of the legs and scurvy].

Old people living alone and in poverty are most at risk for developing scurvy, but the diagnosis may be missed unless the physician is aware of it. A 42-year-old immigrant living in poverty was treated surgically at the Hôtel-Dieu Hospital in Montreal for a compartment syndrome of the leg. She had ecchymoses on the skin of the leg and an interstitial hematoma in both posterior compartments. Three weeks later, she was readmitted with more swelling in the leg, gingivitis, hemorrhagic folliculitis, petechiae and gastrointestinal hemorrhage. Her response to vitamin C was remarkable and confirmed the diagnosis of scurvy.

Adult↗
PubMed1986

Chondromalacia of the fabella: a case report.

A 46-year-old man suffered from chondromalacia of the fabella after minor trauma to the back of the knee. The condition was misdiagnosed initially as fasciitis, tendinitis, a Baker's cyst and a herniated lumbar intervertebral disc. Pathognomonic signs were pain behind the lateral femoral condyle on palpation and compression of the fabella and also on passive extension of the knee. Conservative management was unsuccessful. Excision of the fabella gave quick and lasting relief.

Cartilage Diseases↗
PubMed1985

Complementary role of wrist arthrography in non-union of scaphoid fractures.

Plain radiographs and wrist arthrography of 11 patients with non-united fractures of the scaphoid were retrospectively studied. The arthrographic feature of non-union was found to be opacification of the fracture line and, secondly, of the midcarpal compartment, whereas in patients with fibrous or cartilaginous union no opacification of the fracture line was obtained. There was no single plain radiographic feature that could distinguish non-union from fibrous or cartilaginous union.

Adolescent↗
PubMed1982

Proteins synthesized by rabbit reticulocyte membrane-bound ribosomes.

Rabbit reticulocyte membrane-bound ribosomes liberated by deoxycholate treatment contain degraded forms of ribosomal and messenger RNA. This degradation occurs after the liberation of the ribosomes from the membranes by the detergent because intact birosomal and messenger RNA can be extracted from washed membranes by phenol treatment. Increasing the ionic strength of the detergent buffer prevents this RNA degradation and allows the recovery of membrane-bound ribosomes capable of protein synthesis. Comparison of the proteins synthesized in vitro by the polyribosomes shows that the main protein produced by both free and membrane-bound ribosomes is globin. However, the two types of polyribosomes could be distinguished by the nonglobin proteins they produce.

Animals↗
PubMed1981

Turnover rates of phosphoryl groups in ribosomal proteins of Physarum polycephalum. Evidence for two different mechanisms.

The rate of phosphate exchange in individual ribosomal proteins of Physarum polycephalum was determined in vivo. It was observed that the phosphoryl groups of S3, the major phosphoprotein, had a turnover rate of 1.5% per minute. The phosphoryl groups of proteins L1, L20 and L24 were stable. These results show that the phosphorylation of ribosomal proteins is regulated by at least two different mechanisms. The rapid turnover of phosphoryl groups of the major phosphoprotein is in agreement with the general observation that the phosphate content of this protein is modulated by the physiological state of the cells and possibly involved in the regulation of ribosome activity. The absence of phosphate exchange in acidic proteins suggests that these groups could play a structural role in the ribosome functions.

Adenosine Triphosphate↗
PubMed1976

Free and membrane-bound ribosomes. VI. Distribution of free sulphydryl groups in rabbit reticulocyte ribosomes.

When free and membrane-bound ribosomes were titrated with N-ethyl[14C]-maleimide or 5,5'-dithio-bis(2-nitrobenzoic acid), 25 free SH groups were detected in polysomes, 10 in the 60 S subunit and 14 in the 40 S subunits. When individual ribosomal proteins were analysed, one large subunit protein L14 was found to be strongly labelled compared to the other ribosomal proteins, in polysomes, monosomes and subunits. However, two additional protein spots from the small subunit were more radioactive in monosomes and subunits than inpolysomes. In all these studies, it was found that the distribution of free SH groups in free and membrane-bound ribosomes was identical except for the few proteins which were reported before to be specific for free or membrane-bound ribosomes (Fehlmann, ., Bellemare, G. and Godin, C. (1975) Biochim. Biophys. Acta 378, 119-124 and Fehlmann, M., Bellemare, G. and Godin, C. (1975) FEBS Lett. 59, 8-12).

Animals↗
PubMed1975

Free and membrane-bound ribosomes. II. Two-dimensional gel electrophoresis of proteins from free and membrane-bound rabbit reticulocyte ribosomes.

Free and membrane-bound polysomes were isolated from rabbit reticulocytes. The membrane-bound polysomes were liberated form the membrane with deoxycholate. Monosomes were prepared from the two types of polysomes by incubation with puromycin. The ribosomal proteins were extracted and analyzed by two-dimensional gel electrophoresis. Two proteins of the large subunit, L11 and L17 present in the free monosomes were not found in the membrane-bound monosomes. On the other hand, four additional spots were found in the protein pattern of the membrane-bound monosomes.

Animals↗
PubMed1975

Comparison of the effect of various methods of dissociation on the protein composition of rat liver ribosomal subunits.

Rat liver ribosomes were dissociated into subunits using EDTA, sodium pyrophosphate, high concentrations of KC1, as well as by incubation with puromycin in presence of 0.5 M KC1. The subunits obtained were analyzed using the density gradient centrifugation technique and their ribosomal proteins were separated by means of two-dimensional polyacrylamide gel electrophoresis. The ribosomal protein patterns of the two subunits isolated using each of the dissociating method were compared to the protein patterns of monosomes prepared by puromycin treatment alone. Our results revealed that the use of chelating agents to dissociate the ribosomes resulted in the loss of some ribosomal proteins from the small subunit. On the other hand, the use of KC1 in high concentrations to dissociate the ribosomes did not appear to cause any major loss of proteins from the ribosomes except for some acidic proteins.

Animals↗
PubMed1971

The Fate of l-Phenylalanine Fed to Germinating Pea Seeds, Pisum sativum (L.) var. Alaska, during Imbibition.

Radioactive l-phenylalanine-l-(14)C or -U-(14)C was fed to pea seeds during imbibition. More than 95% was imbibed. Less than 1% of the radioactivity was respired as CO(2). Of the radioactivity taken into the embryos, 80% was still in the cotyledons by 3 days. About half of this was unchanged phenylalanine: 5% free, 10 to 20% in soluble proteins, 1 to 6% in cell wall proteins, and 14% released by mild acid hydrolysis. No other radioactive amino acid was found. About 0.3% of the radioactivity was identified as free caffeic, ferulic, and coumaric acids or their glycosides, and a further 5% was released by mild acid hydrolysis into a phenolic acid fraction. About half of the radioactivity in the cotyledons was lost in the fractionation procedures.About 20% of the radioactivity was found in the shootroot axes to which it had been translocated. Phenylalanine accounted for nearly all the identifiable radioactivity: about 1% free, 1 to 3% in soluble proteins, 1 to 5% in cell wall proteins, and 1% bound to insoluble fractions from which it was released by mild acid hydrolysis. About 1% was released into a phenolic acid fraction. About half of the radioactivity in the shoot-root axes was lost in the fractionation procedures.

Journal Article↗