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Biomedical subjects

C Glad

Publications and source records attributed to C Glad.

5 recordsLinked to original sources
PubMed1989

Cross-reactivity of mouse monoclonal antibodies produced by in vitro or in vivo immunization.

The effect of different immunization schemes on the resulting antibody specificity was investigated. The cross-reactivity of monoclonal antibodies produced by in vitro vs. in vivo immunization was tested, using a solid phase enzyme immunoassay. Ten different monoclonal antibodies were tested against 15 different antigens. There was no difference in cross-reactivity between the two types of antibodies when tested against antigens coated onto the plastic wells in a buffer solution. When the antigens were dried onto the plastic wells the IgM monoclonal antibodies produced by in vitro immunization exhibited a somewhat different reactivity pattern. However, the assay design was shown to be of more importance than the immunization procedure when determining antibody specificities.

Animals↗
PubMed1986

The relationship between metal ion content and mitogenicity of PHA-L4 isolectin as determined by agarose electrophoresis.

The lectin (PHA) from Phaseolus vulgaris is a tetrameric metalloprotein which exists as 5 different isolectins. An agarose electrophoretic assay is described that can distinguish between metallized and demetallized PHA. The assay has been employed for the analysis of 5 different preparations of the isolectin PHA-L4, and the preparations have subsequently been tested for their ability to induce lymphocyte transformation. A reverse correlation was found between the relative amount of irreversibly demetallized isolectin, as determined by agarose electrophoresis and the mitogenic activity of the preparation. The degree of irreversible demetallization seemed to be dependent on the preparation method used. One method gave 25% and another 100% demetallization of the isolectins. The assay was found to be a rapid and simple way to test the relative amount of mitogenically active lectin present in a preparation.

Calcium↗
PubMed1986

Streaming potential--a general affinity sensor.

Electrokinetic phenomena, such as streaming potential or streaming current, have so far been used for studies of unspecific adsorption of ionic compounds on various materials. This paper shows that streaming potential measurements can also be used for studies of biospecific interactions. Several different interactions were studied, e.g. lectin-carbohydrate, IgG-Protein A. Regardless of the type of interaction an increasing change in streaming potential was obtained with increasing concentration of the interacting molecule. The authors also observed a correlation between streaming potential and affinity constants for carbohydrate-induced desorption of Concanavalin A from partially hydrolyzed Sephadex G50. In conclusion, it is shown that streaming potential measurements can be used to study molecular interactions, and to determine concentrations and relative binding constants of interacting molecules.

Antigen-Antibody Complex↗