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C Giaume

Publications and source records attributed to C Giaume.

58 records · Page 4Linked to original sources
PubMed1982

Ammonium sulfate induced uncouplings of crayfish septate axons with and without increased junctional resistance.

Cytoplasmic pH (pH1) of crayfish lateral giant axons was monitored using antimony microelectrodes placed near septate junctions and variations of internal pH was induced by short applications of ammonium sulfate in the perfusing bath of the preparation. This treatment produced a rapid cell alkalinization followed, after wash, by acidification rebound. Simultaneously, two successive phases of uncoupling of the septate junction occurred; they had the same time course as those of their associated pH1 movements. Calculation of the electronic coupling parameters indicated that, during alkalinization, coupling was accompanied by an increased axonal membrane conductance (the intimate origin of which was beyond the scope of this study) and resulted from a shunt of the gap junctions; the resistance proper of the latter was unaffected; thus involvement of Ca2+ was ruled out and uncoupling was only an indirect consequence of the electrotonic junction's network configuration. In contrast, and as expected from previous investigations, the junctional membrane resistance was increased during the second phase of cytoplasmic acidification. Evidence that uncoupling can be brought about by a non-junctional membrane increased permeability raises questions about some of the criteria commonly used during investigations of electrotonic transmission.

Ammonium Sulfate↗
PubMed1980

Uncoupling of invertebrate electrotonic synapses by carbon dioxide.

Cell acidification was obtained by exposing to carbon dioxide the lateral giant axon of the crayfish and the buccal ganglion of Navanax inermis; electrophysiological measurements show that the resulting intracellular pH uncouples neurons and that this uncoupling is the consequence of increased junctional resistance at the level of the gap junctions. Conditions under which the uncoupling is reversible are also described.

Animals↗
PubMedDate not supplied

Microglia and astrocytes may participate in the shaping of visual callosal projections during postnatal development.

In the adult cat, axons running through the corpus callosum interconnect the border between the visual cortical areas 17 and 18 (A17 and A18) of both hemispheres. This specific pattern emerges during postnatal development, under normal viewing conditions (NR), from the elimination of initially exuberant callosal projections. In contrast, if the postnatal visual experience is monocular from birth (MD), juvenile callosal projections are stabilised throughout A17 and A18. The present study aimed at using such a model in vivo to find indications of a contribution of glial cells in the shaping of projections in the developing CNS through interactions with neurones, both in normal and pathological conditions. As a first stage, the distribution and the morphology of microglial cells and astrocytes were investigated from 2 weeks to adulthood. Microglial cells, stained with isolectin-B4, were clustered in the white matter below A17 and A18. Until one month, these clustered cells displayed an ameboid morphology in NR group, while they were more ramified in MD animals. Their phenotype thus depends on the postnatal visual experience, which indicates that microglial cells may interact with axons of visual neurones. It also suggests that they may differentially contribute to the elimination and the stabilisation of juvenile exuberant callosal fibres in NR and MD animals respectively. Beyond one month, microglial cells were very ramified in both experimental groups. Astrocytes were labelled with a GFAP-antibody. The distributions of connexins 43 (Cx43) and 30 (Cx30), the main proteic components of gap junction channels in astrocytes, were also investigated using specific antibodies. Both in NR and MD groups, until 1 month, GFAP-positive astrocytes and Cx43 were mainly localised within the subcortical white matter. Then GFAP, Cx43 and Cx30 stainings progressively appeared within the cortex, throughout A17 and A18 but with a differential laminar expression according to the age. Thus, the distributions of both astrocytes and connexins changed with age; however, the monocular occlusion had no visible effect. This suggests that astrocytes may contribute to the postnatal development of neuronal projections to the primary visual cortex, including visual callosal projections.

Animals↗