b-->s gamma decay and right-handed top-bottom charged current.
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Journal Article↗
Biomedical subjects
C Gao
Publications and source records attributed to C Gao.
Glueball production from the quark-gluon plasma.
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Journal Article↗
[Inclusion compounds of jiuxin oil with beta-cyclodextrin].
The inclusion compounds of Jiuxin Oil with beta-cyclodextrin were prepared. The influence of temperature, ratio of host and guest molecules, stirring time on inclusion were observed. The quantitative determination was performed by gas chromatography. Inclusion compounds of Jiuxin Oil were confirmed by differential scanning calorimetry and powder X-ray diffractometry.
Cyclodextrins↗
[The diagnosis and treatment of benign esophagotracheo-bronchial fistula: a report of 26 cases].
Twenty six patients with esophagotracheal fistula or esophagobronchial fistula were treated from 1960 to 1991. There were 18 males and 8 females with age ranging from 19 to 69. Trauma and complication of esophageal diverticulum were the main causes of fistula. Among 23 patients surgically treated, 10 underwent direct repair, and 13 either closure of esophageal defect or tracheal or bronchial defect. The concomitant procedures were permanent tracheostomy, tracheal resection and reconstruction, pulmonary resection, thoracoplasty esophagectomy, and esophagogastric anastomosis. All patients resumed normal eating. Complications included paralysis of recurrent nerves, empyema, injury and ligation of subclavian artery, dehiscence of tracheal anastomosis, and contralateral pneumohydrothorax in each patient. Prognosis of 3 nonsurgical treatments of fistulas was poor. Surgical intervention should be done as soon as the diagnosis is established in order to minimize pulmonary complication.
Adult↗
LHRH neuronal subtypes have multiple origins in chickens.
Previous studies indicate that LHRH neurons of the septal-preoptic area originate in the olfactory epithelium. In the current study, we found that LHRH neurons entering the telencephalon from the olfactory nerve were not continuous with LHRH neurons in the thalamus. In addition, ablation of the olfactory placode eliminated LHRH neurons in the telencephalon but did not eliminate LHRH neurons in the thalamus. We propose that there are at least two sites of LHRH neuron production, the olfactory epithelium and the diencephalon.
Animals↗
[A brief history of cardiologoy in Western medicine].
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Cardiology↗
[Effects of moxibustion on the function of MDR gene product, P-glycoprotein (P-170)].
The experiments were performed on BABL/c mice with S-180R adriamycin resistant tumor cells. This animal model was used to analyze the drug accumulation in the S-180R cells by flow cytometer. The drug accumulation presents the pump activity of multidrug resistance (MDR) gene product P-glycoprotein (P-170) in the cell membrane, A weak inhibition was found when moxibustion at Guan-Yan point alone. And a very significant inhibition was observed in the presence of low-dosage of verapamil, but not at high dose. This study may develop a new way to research the mechanism of acupuncture and moxibustion at molecular level, and may be useful to overcome the anticancer drug resistance.
ATP Binding Cassette Transporter, Subfamily B, Mem↗
[Microdissection of human chromosome 1].
This work began with chromosome preparation and microdissection. Booster IRS-PCR was developed to amplify DNA dissected form human chromosome 1. The accuracy of microdissection was demonstrated by chromosomal in situ suppression (CISS)-hybridization. The amplified DNA fragments were ligated to pUC 18 vector at SmaI site and the average size of the inserts was about 450bp.
Chromosomes, Human, Pair 1↗
[Case report of pulmonary histoplasmosis].
A case of pulmonary histoplasmosis in Beijing was reported Patient's CT scan showed naly patchy consolidation partly with small cavitations and hilar silhouette enlarged in both lungs. On pathologic examination, Histoplasma Capsutatums were found in the lung tissures by percutaneous lung biopsy. Skin rash had developed in the course. After treated by fluconaxole, most of the lung lesions and skin rash disappeared.
Adult↗
[Chromosome abnormality and dominant lethality among the F1 generation of mice after gamma-ray irradiation].
This paper reports on the relationship between chromosome abnormality or dominant lethality and dosage of 0.15-1.56 Gy 60Co gamma-ray irradiation in the F1 generation of mice. The irradiated female mice were mated at 7-12 days after irradiation with unirradiated or irradiated at 22-28 days after irradiation. The fetuses aged 9.5-11 days were recovered from the uterus and lethality and chromosome aberrations were investigated. Results suggested that the rate of fetus with chromosome abnormality increased with dose, and so did dominant lethality rate, and both could fit linear relationship. Increase of translocation carriers in the F1 mice was observed at the dose level of over 1.0 Gy.
Animals↗
Effects of low level prenatal 60Co gamma-irradiation on postnatal growth and behavior in mice.
Pregnant Kun Ming strain mice were exposed to a total dose of 0, 0.1, 0.2, or 0.4 Gy from 60Co gamma-rays from the 13th to the 18th days of gestation. An overall delay of the appearance of two physiologic markers (pinna detachment, eye opening) and the age of acquisition of four reflexes (surface righting, air righting, auditory startle, visual placing) was observed in offspring exposed to 0.2 or 0.4 Gy in utero. Postnatal growth retardation, shortened length of hanging time, inhibited exploratory activity in the hole board test (decreased number of head-dipping), and hyperactivity in the open field test (shortened latency to leave the center area and increased number of squares entered) were also found among the offspring exposed to 0.2 Gy or more in utero. The results indicate that 0.1-0.2 Gy may represent a threshold range in mice for certain physiologic and behavioral effects resulting from continuous exposure to 60Co gamma-rays on the 13th-18th days of gestation.
Animals↗
[Cytogenetic and molecular genetic study on a female with complex translocation of t(Y;15) and t(14;21)].
A 27-year-old female having a baby with Down's syndrome was found to have a translocation t(14;21) and an acrocentric marker chromosome 15. The short arm of the marker was darkly stained in both G- and C-banded preparations, and had the normal Ag-NOR, but showed a homogeneously stained region in the distal part of the marker. By means of QM staining, the variant was found fluorescent, and Y-body like was found in about 9% interphase nuclei, which suggested that there might exist Y chromosome material in genomic DNA of proband. Dot blot hybridization using DNA probe pY3.4 derived from the heterochromatic region on the long arm of Y chromosome demonstrated that the proband carried Y chromosome material in her genome. Further, by in situ hybridization with the same probe, it showed that the variant of the marker had specific autoradiographic silver grains. So, the karyotype of the proband indicated that the combination of conventional banding analysis with chromosome-specific probe was of great significance in studying the micro-aberration of human chromosome.
Adult↗
[Molecular and clinical cytogenetic studies of a family with a 22p+ marker chromosome].
A male with gonadal dysgenesis and a 22p+ was observed. Molecular and Clinical Cytogenetic studies have been carried out on the members of the family. The results showed that there was a 22p+ marker chromosome transmitted from the maternal grandmother of the proband to 6 members of this family. Its short arm showed a homogeneously dull stained region in C-banded preparations and a narrow dark or light stained band in R-, G-banded preparations respectively. A large Ag-band or double NORs was also observed on p+. The chromosomal in situ hybridization with tritium labelled rRNA gene probe demonstrated that the distribution of the silver grains was along the entire p+ of the marker chromosome. The number of silver grains on the short arm of the p+ was 3.9 times as that of any other normal acrocentric chromosomes. Two cases of female with repeated spontaneous abortions and 2 cases of male with gonadal dysgenesis were found by family study. Our studies, combining with previous literatures suggested that these abnormalities were probably in association with p+ marker chromosome.
Adult↗
Yuan, Shao, and Gao reply.
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Journal Article↗
New plasmid vector and host system for genetic engineering in Bacillus sphaericus.
A new plasmid, pNQ116, was constructed in Bacillus sphaericus by cloning a promoter fragment from B. sphaericus Ts-1 into pNQ112. The plasmid (CmrKmr, 5.23 kb) contains a restriction endonuclease polylinker used for cloning foreign genes, and its cat-86 gene is expressed at high levels from the Ts-1 promoter. This plasmid vector has been transformed into B. sphaericus AS 1.270, AS 1.465, AS 1.469, and 2362, at frequencies of 10(2)-10(3) transformants per microgram of DNA, and is maintained stably under nonselective conditions in these host strains. The presence of pNQ116 in B. sphaericus 2362 does ot interfere with the mosquito larvicidal activity of the organism.
Animals↗
[A modified staining method for examining Y chromatin].
A modified staining method for Y chromatin is reported here which proved to be rapid and efficient for sex differentiation diagnosis. Samples were fixed in methanol: acetic acid (3:1) solution and stained with dyes in McIlvaine buffer. Y chromatin in buccal smears from normal males could be clearly revealed by this method. With this modified method and X-chromatin examination, one testicular feminization was found during examination of 1342 athletes for the 11th Asian Games.
Cheek↗
[Effect of electroacupuncture on myocardial oxygen metabolism and pH of coronary sinus blood during experimental angina pectoris].
The experiments were performed on 30 healthy mongrel dogs, intubation was taken in the left anterior descending coronary (LAD), the blood in carotid was transported to LAD through a peristalic pump, the blood flow was reduced to 3-5 ml/min, thus acute myocardial ischemia was produced. Basing on this condition, 0.1-0.16 ml bradykinin (2 ug/ml) was given into LAD coronary before recording to produce angina pectoris. The effects of electroacupuncture (EA) at "Neiguan" area on myocardial oxygen metabolism, pH of coronary sinus blood and myocardial contractile force were observed (EA intensity 5 volts, frequency 1-20 Hz). The results are as follows: 1. EA could reduce obviously A-V difference of blood oxygen capacity (Ca-vO2) and the rate of myocardium extracting oxygen (O2E), thus reduced obviously oxygen consumption of ischemic myocardium. 2. EA could reduce V-A difference of carbon dioxide partial pressure (Pv-aCO2), prevent the decrease of pH of coronary sinus blood (PHv), this indicated that EA could prevent accumulation of acidic metabolic products. 3. EA could increase myocardial developed tension (DT) of ischemic area, strengthen myocardial contractile force of ischemic area. Above results indicated that EA could reduce oxygen consumption of ischemic myocardium, prevent the decrease of pH of coronary sinus blood, thus myocardial cell acidosis was prevented, myocardial contractile force was strengthened. It might be the mechanism of acupuncture treating coronary heart disease.
Angina Pectoris↗
Molecular cytogenetic study of short arm aberrations in human D, G group chromosomes.
Six cases of Dp+/Gp+, 10 cases of D/G translocation, 1 case of supernumerary marker chromosome, and 1 case of Yqs were studied using molecular and cytogenetic techniques. The Ag-NOR frequencies of the Dp+ and Gp+ groups were found to be higher than those of normal controls, while their satellite association frequencies were lower. Autoradiographic silver grains were not significantly distributed along the p+ part of the marker chromosome as revealed by chromosomal in situ hybridization using an rRNA probe. This result differs from our previous report. It is suggested that there might be different mechanisms for the formation of p+ on acrocentric chromosomes. D/G translocation cases were found to have lost their NOR. A study of supernumerary marker chromosomes and Yqs cases suggested that the marker chromosome and Yqs exerted no phenotypic effect. The mechanism of their formation is discussed.
Adolescent↗