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Biomedical subjects

C G Cumming

Publications and source records attributed to C G Cumming.

26 records · Page 2Linked to original sources
PubMed1981

Isolation of Bordetella pertussis from swabs.

Four varieties of swabs held in plastic containers and three swab transport media kits, all obtainable in the United Kingdom, were tested to assess their effectiveness in the isolation of Bordetella pertussis. The highest isolation rates up to 48 hours were obtained with Dacron swabs held in plastic containers. Swab-transport media kits proved unsatisfactory. B pertussis may have a stronger affinity to Dacron than to cotton-wool, and pernasal swabs should be made of Dacron to improve the recovery of B pertussis.

Bacteriological Techniques↗
PubMed1981

Optimal methods for the isolation of groups A, B, C and G streptococci.

The recovery of beta-haemolytic streptococci on laboratory media over a 48-hour period was studied from a variety of swabs held in plastic containers only, and from those held in transport media, stored either at room temperature or at 4 degrees C. The best results were obtained from swabs not held in transport medium and of these plain cotton-wool swabs consistently produced optimal results. The percentage recovery over 48 hours was maintained satisfactorily only when the swabs were kept at 4 degrees C.

Bacteriological Techniques↗
PubMed1981

Immunochemical studies on the cell-wall antigen of group B streptococcus, type Ib.

A polysaccharide antigen of group B streptococcus type Ib was extracted from sodium dodecyl sulphate-treated cell walls by trichloroacetic acid. In crossed immunoelectrophoresis the polymer reacted with specific antisera to serotypes Ia, Ib and Ic of group B streptococci and with commercial grouping antiserum to give one precipitin line. However, the antigen did not react with antisera to types II and III. The evidence suggests that this polymer confers the group specificity to type Ib of group B streptococci. The polysaccharide, after purification on DEAE-cellulose, was shown to consist of rhamnose, glucosamine, galactose, glucose, sialic acid and a trace of phosphorus.

Antigens, Bacterial↗
PubMed1980

Use of the Streptosec test for grouping beta-haemolytic streptococci.

The Streptosec test, which embodies the coagglutination principle for grouping beta-haemolytic streptococci, was used against 72 streptococci previously grouped by precipitin methods. Only two of the 72 strains failed to react. The test is easy to manipulate, represents a considerable saving in time and effort, and produces results with an acceptable degree of accuracy.

Agglutination Tests↗
PubMed1979

Evaluation of bacteriological swabs and transport media in the recovery of group B streptococci on laboratory media.

The survival of group B streptococci on a variety of swabs, held either in their containers or in transport media for periods of up to 48 hours, at room temperature and at 4 degrees C, has been assessed. Results indicated that holding swabs in transport media did not favour prolonged survival of the streptococci and that the yield of organisms was much greater from swabs held in their ordinary plastic tubes. A holding temperature of 4 degrees C rather than room temperature is recommended if any delay in plating out swabs is anticipated.

Bacteriological Techniques↗