Biomedical subjects
C Finaz
Publications and source records attributed to C Finaz.
[Increasine in the rate of cell hybridization by the cytotoxic action of strophanthin (ouabain)].
Incorporation of ouabain (G-strophantin) in the culture medium 24 h after that of Sandai virus, at a final concentration of 2.10(-6)M/1, increases considerably the yield of man/mouse or primate/mouse cellular hybridization.
Assignment of the ABO-N-P-AK1 linkage group to chromosome 9 in man-hamster hybrids.
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[Karyotyping of the cercopithecus (Cercopithecus aethiops). Banding and nomenclature].
The karyotype of Cercopithecus aethiops was analysed by RHG, GTG, and C banding, and a band nomenclature is proposed.
Chromosome no. 1 of man and chimpanzee: identity of gene mapping for three loci: PPH, PGM1, and Pep-C.
Cytogenetic and biochemical analysis of 10 independant Chimpanzee-Mouse cell hybrids and of 18 subclones of one of these showed that PPH, PGM1 and Pep-C are localized on the Chimpanzee chromosome homologous to the human chromosome No. 1.
[Gene localization in the chimpanzee (Pan troglodytes). Comparison with the factor mapping of man (Homo sapiens)].
Ten independant cellular hybrids were obtained from Chimpanzee (Pan troglodytes) fibroblasts and the murine cell line C11D. The comparison of electrophoretic and cytogenetic studies showed that 9 markers with known localizations in Man could also be localized on the homologous chromosomes of the Chimpanzee: pyrophosphate hydratase (PPH), phosphoglucomutase-1 (PGM1), and peptidase-C (Pep-C) on the No. 1; malate dehydrogenase MDH(NAD) on the No. 2; lactico dehydrogenase-A (LDH-A) on the No. 11; lactico dehydrogenase-B (LDH-B) on the No. 12; thymidine kinase (TK) on the No. 17; superoxide dismutase-1 (SOD-1) on the No. 21; glucose-6-phosphate dehydrogenase (G6PD) on the X. The localization of mannose phosphate isomerase (MPI) on the No. 7 could be excluded. One discrepancy between Chimpanzee and Man was noted: the localization of superoxide dismutase-2 (SOD-2) on the 6 is excluded.
[Probable existence of hexosaminidase C locus located on chromosome 7 in man].
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Identification of individual chromosomes in the human karyotype by their banding pattern after proteolytic digestion.
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Comparison of banding patterns of human chromosomes obtained with heating, fluorescence, and proteolytic digestion.
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[2 familial translocations occurring together in each of 2 sisters, one balanced, the other partial trisomic 10q].
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[Study of cellular metachromasia in fibroblast cultures].
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[Demonstration of the fine structure of human chromosomes by enzymatic digestion (especially pronase)].
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[The human karyotype after treatment with alpha-chymotrypsin].
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In vivo regulation of rat epididymal proteins by retinoids: analysis by two-dimensional electrophoresis.
The role of retinoids in the regulation of epididymal fluid protein expression was investigated. We compared the patterns of two-dimensional electrophoretic gels of proteins from luminal fluids, cytosols and spermatozoa (from control rats only) of control, retinoid-depleted, retinoid-depleted retinoic acid-complemented and retinoid-depleted testosterone-supplemented rats. This study compared the luminal fluid patterns from the 4 diets and observed 13 proteins whose expression was dependent on nutritional status. Eight were either absent or very weakly expressed in retinoid-depleted animals only, while their presence was obvious in control rats and in the retinoid-deficient retinoic acid- and testosterone-complemented groups. The expression of 8 proteins was greatly enhanced in retinoid-depleted testosterone-supplemented fluids as compared to control fluids. Five of the regulated proteins seemed to be captured by spermatozoa as they were observed in sperm protein patterns of control rats. These results clearly show that the synthesis of several epididymal proteins is influenced by retinoids. Since testosterone-supplemented animals on retinoid-free diet elicited the same response as retinol and retinoic acid ones, testosterone is likely to be the mediator of retinoid action on epididymal protein synthesis. Nevertheless, the observation of one protein whose expression is stimulated by retinoic acid only and is totally independent of testosterone also favors the direct influence of this retinoid.
[Gamete recognition in mammals: sperm and zona pellucida interactions].
Association of sperm with the acellular protective envelope of the oocyte, the zona pellucida, and their penetration is a determinant step in fertilization process. It is at this stage that species barriers take place to prevent cross fertilizations. This association is dependent upon binding of ZP3 oligosaccharides to specific sperm receptors. Their activation triggers the acrosome reaction via transduction pathways and release of proteolytic enzymes that dissociate the zona pellucida network. Then, secondary binding to zona pellucida components allows sperm to penetrate and cross the zona pellucida barrier. Several sperm surface proteins are putative receptors for zona pellucida partners. Repercussion of these studies on human fertility are discussed.