Reference method for serum total cholesterol measurement: does substituting enzymatic step for Liebermann-Burchard reaction improve specificity?
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Biomedical subjects
Publications and source records attributed to C Ferrero.
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The development of a curved crystal monochromator of the Laue type for energy-dispersive X-ray absorption spectroscopy is presented. The quality of the X-ray absorption spectra at high photon energies is compared with spectra measured with silicon crystals in the more frequently used Bragg geometry. In the Bragg case, an asymmetric broadening of the reflectivity profile leads to strong distortions of the near-edge fine structure and to a reduction in spectral resolution. The reflectivity profiles of fiat and curved crystals for Laue and Bragg geometry have been calculated using dynamical theory and are compared with experimental data. The new optics have been used for in situ time-resolved X-ray absorption spectroscopy. An example of the application of the technique for the characterization of a Pd catalyst is given. The X-ray absorption fine structure at the Pd K-edge has been measured during the activation and during the heterogeneous catalytic oxidation of carbon monoxide.
The aim of this study is to present a relatively rare case of paradoxical arterial embolism, found in a patient who was sent to us for serious pulmonary embolism. Taking into account that the foramen ovale, despite being functionally competent, remains anatomically patent in 30% of the adult population, we cannot neglect the possibility of a paradoxical embolism, in the presence of a sudden embolic limb ischemia unless heart pathology or aortic lesions can be held responsible. Furthermore it must not be forgotten that deep venous thrombosis in the lower limbs or in the pelvic plexus may go unobserved on a purely clinical evaluation.
The purpose of the present study is to underline the importance of the systematic search for iliocaval venous compression on the part of an aortoiliac aneurysm in the face of a clinical picture of suspected deep venous thrombosis. Early diagnosis of this syndrome, although rare in everyday experience, is of decisive importance, as is every other clinical sign of aneurysmal pathology prior to rupture. Correct, systematic diagnostic exclusion procedure, which is capable of leading to certain diagnosis in all cases is therefore necessary.
A toxoplasma gondii antigen, has been obtained by sonication, separated by SDS gel electrophoresis system in 10% acrylamide and blotted onto nitrocellulose paper (Western Blot). The antigen has been tested against: serum samples from subjects T. gondii negative; serum samples from patients with acute Toxoplasma infection; serum samples from subjects who had serologic evidence of infection with T. gondii, but IgM negatives. IgG and IgM immune response has been evaluated. Gel stained with Coomassie Blue revealed 48 protein fractions; only twenty fractions reacted as antigen. In particular, we have observed that only one protein fraction has been recognized by IgM antibodies present in the serum of patient with acute Toxoplasma infection (with lymphoadenopathy); some protein fractions have been recognized only by IgG antibodies; some protein fractions have been recognized in the same time by IgG and IgM antibodies in the same serum sample.
The results obtained from serologic diagnosis on patients with suspicion of toxoplasmic lymphadenitis are referred. The data concerning specific IgM, obtained on whole serum and after gel filtration, are compared. The IgM fraction obtained after gel filtration has been used for AD, ELISA IgM and IFA test; the reliability of the different tests has been evaluated.
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In 88 cases out of 126 (70%), antibodies to rubella were found by inhibition of viral hemagglutination in wild pigeons collected in the area of Nice. When experimentally infected with live virus, the rubella negative pigeons develop HI antibodies, thus showing that the virus replicates in these birds. The epidemiological meanings of these findings are considered.
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In a series of 38 complete right bundle branch blocks (RBBB) (17 "idiopathic" and 21 cardiopathies), auscultation and phonocardiograms disclosed a normal split second heart sound (S2 = A2-P2 less than or equal to 0.045 sec) in 27 and a wide split S2 (A2-P2 greater than or equal to 0.06 sec) in only 11 cases. Analysis of the initial and terminal vectors constructed from ECGs recorded at 250 mm/sec shows no valid correlation between a transseptal mode of depolarization of the right ventricle and the presence of either a normal or wide split of the 2nd heart sound. A review of the mechanisms generating the 2nd sound and discussion of the additional factors which may influence the duration of A2-P2 in RBBB (start of activation, length and quality of right ventricular systole, impedance of pulmonary arterial bed, possible delay of 2nd aortic sound) explain why wide splitting of the 2nd sound in RBBB is the exception rather than the rule.
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