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Biomedical subjects

C Farmer

Publications and source records attributed to C Farmer.

At least 37 records · Page 2Linked to original sources
PubMed1998

Improving the care of patients treated with hemodialysis: a report from the Health Care Financing Administration's ESRD Core Indicators Project.

To determine the impact of a quality improvement intervention on dialysis care delivered to hemodialysis patients, we studied 213 hemodialysis facilities in North Carolina, South Carolina, and Georgia. Dialysis adequacy measurements made on two random samples of 30 patients per treatment center, or all patients if fewer than 30 were treated, selected in October 1994 (preintervention) and October 1995 (postintervention) were used to estimate the facility mean urea reduction ratio (URR) and the proportion of patients with a mean URR less than 50%. The 10% of facilities (n = 22) with the highest proportion of patients with a mean URR less than 50% in the facility at preintervention were selected for an intervention that included feedback of facility-specific mean URR, educational programs, a quality improvement workshop, and monitoring until improvement was attained. Changes between preintervention and postintervention facility mean URR and proportions of patients with a URR less than 60% and 65% were used to assess the impact of the intervention. After 1 year, the mean URR had increased an average of 7% in intervention centers compared with an increase of 1.4% (P < 0.001) in the remainder of the treatment centers in the Network. There was an average reduction of 17.2% in the proportion of patients with a URR less than 65% in intervention centers compared with 4.8% in the other facilities (P < 0.001). Comparable reductions in the proportion of patients with a mean URR of less than 60% were 16.2% in intervention centers and 2.0% in comparison facilities (P < 0.001). After controlling for facility case mix and other characteristics, the intervention was independently associated with an absolute 2.4% increase in facility-specific mean URR. We conclude that the intervention was associated with improvement in hemodialysis care.

Ambulatory Care Facilities↗
PubMed1998

Air-breathing during activity in the fishes amia calva and lepisosteus oculatus

Many osteichthyan fishes obtain oxygen from both air, using a lung, and water, using gills. Although it is commonly thought that fishes air-breathe to survive hypoxic aquatic habitats, other reasons may be more important in many species. This study was undertaken to determine the significance of air-breathing in two fish species while exercising in oxygen-rich water. Oxygen consumption from air and water was measured during mild activity in bowfin (Amia calva) and spotted gar (Lepisosteus oculatus) by sealing a fish in an acrylic flume that contained an air-hole. At 19-23 degreesC, the rate of oxygen consumption from air in both species was modest at rest. During low-level exercise, more than 50 % of the oxygen consumed by both species was from the air (53.0+/-22.9 % L. oculatus; 66.4+/-8.3 % A. calva).

Journal Article↗
PubMed1998

Bromocriptine given orally to periparturient of lactating sows inhibits milk production.

Gilts were assigned as controls (CTL, n = 6) or received orally 10 mg of bromocriptine thrice daily from d 110 of gestation until farrowing, (BRPP, n = 7), from d 1 to 6 postpartum (PP) (BRL1, n = 6), from d 7 to 13 PP (BRL2, n = 7), from d 14 to 20 PP (BRL3, n = 6), or from d 21 to 27 PP (BRL4, n = 6). Weights of pigs were recorded at birth, 24 h later, and on d 7, 14, 21, 28, 31 (weaning), 42, and 56 PP. Jugular blood samples were collected from sows on d 109 of gestation and every other day until farrowing, as well as on d 1, 6, 13, 20, and 27 PP. Behavioral observations of sows and litters were taken every 3 min for a 24-h period beginning 48 h after the onset of the treatments. In experimental sows, bromocriptine induced marked reductions in prolactin levels during treatment (P < .001). Compared to CTL sows, concentrations of IGF-I were higher at d 21 (P = .01) and 28 (P = .003) PP in BRL3 and BRL4 sows, respectively. In bromocriptine-treated sows, weight gain of litters was either drastically reduced or abolished (P < .001) during the week of treatment. Treatments also altered significantly the suckling behavior of pigs at all stages studied. Therefore, the present results strongly suggest that prolactin is essential for the initiation and the maintenance of milk production in sows. Results also indicate that prolactin does not seem to be involved in the maintenance of lactational anestrus during a 4-wk lactation.

Administration, Oral↗
PubMed1997

Continuous ambulatory peritoneal dialysis. Experience in a single renal unit in the UK.

The outcome of 106 patients started on continuous ambulatory peritoneal dialysis (CAPD) in a single renal unit over a 3-year period was reviewed in order to compare our experience with that of other centres. The incidence of peritonitis was not different in diabetics compared with non-diabetics, in the elderly compared with younger patients, and in non-compliant compared with compliant patients. The patients' level of education had a significant effect on the number of days required to train for CAPD. Diabetics and non-compliant patients required more hospitalization. The need for community support increased the incidence of peritonitis.

Adult↗
PubMed1997

High-frequency oscillatory ventilation for adult respiratory distress syndrome--a pilot study.

OBJECTIVE: To evaluate the safety and effectiveness of high-frequency oscillatory ventilation using a protocol designed to recruit and maintain optimal lung volume in patients with severe adult respiratory distress syndrome (ARDS). SETTING: Surgical and medical intensive care units in a tertiary care, military teaching hospital. DESIGN: A prospective, clinical study. PATIENTS: Seventeen patients, 17 yrs to 83 yrs of age, with severe ARDS (Lung Injury Score of 3.81 +/- 0.23) failing inverse ratio mechanical conventional ventilation (PaO2/FiO2 ratio of 68.6 +/- 21.6, peak inspiratory pressure of 54.3 +/- 12.7 cm H2O, positive end-expiratory pressure of 18.2 +/- 6.9 cm H2O). INTERVENTIONS: High-frequency oscillatory ventilation was instituted after varying periods of conventional ventilation (5.12 +/- 4.3 days). We employed lung volume recruitment strategy that consisted of incremental increases in mean airway pressure to achieve a PaO2 of > or = 60 torr (> or = 8.0 kPa), with an FiO2 of < or = 0.6. MEASUREMENTS AND MAIN RESULTS: High-frequency oscillator ventilator settings (FiO2, mean airway pressure, pressure amplitude of oscillation [delta P] frequency) and hemodynamic parameters (cardiac output, oxygen delivery [DO2]), mean systemic and pulmonary arterial pressures, and the oxygenation index (oxygenation index = [FiO2 x mean airway pressure x 100]/PaO2) were monitored during the transition to high-frequency oscillatory ventilation and throughout the course of the high-frequency protocol. Thirteen patients demonstrated improved gas exchange and an overall improvement in PaO2/FiO2 ratio (p < .02). Reductions in the oxygenation index (p < .01) and FiO2 (p < .02) at 12, 24, and 48 hrs after starting high-frequency oscillatory ventilation were observed. No significant compromise in cardiac output or DO2 was observed, despite a significant increase in mean airway pressure (31.2 +/- 10.3 to 34.0 +/- 6.7 cm H2O, p < .05) on high-frequency oscillatory ventilation. The overall survival rate at 30 days was 47%. A greater number of pretreatment days on conventional ventilation (p < .009) and an entry oxygenation index of > 47 (sensitivity 100%, specificity 100%) were associated with mortality. CONCLUSIONS: High-frequency oscillatory ventilation is both safe and effective in adult patients with severe ARDS failing conventional ventilation. A lung volume recruitment strategy during high-frequency oscillatory ventilation produced improved gas exchange without a compromise in DO2. These results are encouraging and support the need for a prospective, randomized trial of algorithm-controlled conventional ventilation vs. high-frequency oscillatory ventilation for adults with severe ARDS.

Adolescent↗
PubMed1997

Presence of a bioactive and immunoreactive growth-hormone-releasing-factor-like substance in porcine placenta.

The present study was conducted to establish whether growth hormone (GH) releasing factor (GRF) is present in porcine placenta. Various concentrations of placental extracts from sows at 90 and 110 days of gestation were added to porcine anterior pituitary cells in culture. The extract-induced GH secretion was compared to that of GRF(1-29)NH2. Experiments were also performed in the presence of the GRF receptor antagonist N alpha-acetyl(D-Arg2-Ala15) rat GRF(1-29)NH2 (10(-7) M). Significant effects on GH production were obtained with increasing concentrations of placental extracts (p < 0.001). This response was significantly diminished in the presence of the GRF receptor antagonist (p < 0.001). The effect of gestational age at the time of collection of the placenta was nonsignificant (p > 0.1). In addition, using a placental extract from 110 days of gestation, an immunoreactive GRF content of 52-63 ng/mg dry weight was detected. Altogether, the detection of an immunoreactive GRF-like substance in porcine placental extracts and the ability of these extracts to stimulate GH release in a concentration-dependent manner, likely by triggering a GRF-receptor-mediated mechanism, support the presence of GRF in this tissue.

Analysis of Variance↗
PubMed1996

Fluorescence spectra of DNA dyes measured in a flow cytometer.

The spectral properties of fluorescent dyes often vary with experimental conditions. Spectral changes contain information about the particles to which the dye is bound and can have practical consequences for the design of flow cytometry experiments. It is therefore desirable to have spectral information obtained under conditions that approach those under which the measurements in a flow cytometer are performed. This report describes the measurement of fluorescence spectra in a flow cytometer. We have attached a monochromator to one of the detector ports of our flow cytometer. The monochromator scans continuously over a range of wavelengths. For each measured particle, the monochromator wavelength is recorded along with the fluorescence intensity at that wavelength. Other fluorescence and scatter signals can also be recorded to distinguish different particle populations within the sample. This method for measurement of fluorescence spectra has advantages over conventional fluorimetry in that it distinguishes between bound and unbound dye and that spectral properties of different particle populations within a single sample can be separately reconstructed. Fluorescence spectra of common DNA dyes bound to chromatin are presented. A shift to the red in Hoechst 33258 fluorescence with increasing dye concentration is demonstrated. The spectra of DNA-bound Hoechst and ethidium bromide are compared with those of the free dyes in solution. Energy transfer between several pairs of DNA dyes is demonstrated using spectral measurements. We also find slight spectral variations between the different human chromosomes dualstained with Hoechst and chromomycin.

Bisbenzimidazole↗
PubMed1996

Tracheal gas insufflation combined with high-frequency oscillatory ventilation.

OBJECTIVES: To determine the efficacy of tracheal insufflation delivered by two different catheter designs on CO2 elimination when used in conjunction with high-frequency oscillatory ventilation. DESIGN: A nonrandomized before and after trial. Each animal served as his own control. SUBJECTS: Ten mongrel dogs weighing 20.9 +/- 1.9 kg. Four animals were assigned to a normal lung group and six animals underwent lung injury by large volume saline lavage. INTERVENTION: Permissive hypercapnia was allowed to occur by selecting oscillator settings that would lead to alveolar hypoventilation. Proximal mean airway pressure was kept constant. Tracheal gas was insufflated at 1 cm above the carina for 30 min periods at gas flows of 5 to 15 L/min. MEASUREMENTS AND MAIN RESULTS: Carinal pressure, hemodynamic parameters (cardiac output, mean arterial pressure, pulmonary artery occlusion pressure), and gas exchange parameters (PaCO2, PaO2, PaO2/FIO2, shunt fraction, D O2) were measured. For the normal dogs, at catheter flow of 15 L/min; the forward thrust catheter increased carinal pressure and Pao2/FIO2 BY 30% (p<.003) and 105% (p<.005), respectively. The forward thrust catheter reduced Paco2 by 40% (p<.04). The reverse thrust catheter increased PaO2/FIO2 by 102% (p<.001) and decreased pressure and PaCO2 by 44% (p<.001) and 34% (p<.003), respectively. For the injured dogs, at catheter flow rate of 15 L/min, the forward thrust catheter increased carinal pressure, PaO2, and PaO2/FIO2 by 6% (p<.001), 23% (p<.001), and 24% (p<.02), respectively. The forward thrust catheter reduced PaCO2 by 29% (p<.002). The reverse thrust catheter increased PaO2 and PaO2/FIO2 both by 11% (p<.02) and reduced carinal pressure and PaCO2 by 23% (p<.001) and 18% (p<.002), respectively. CONCLUSIONS: Tracheal gas insufflation is capable of improving oxygenation and ventilation in acute lung injury when combined with high-frequency oscillatory ventilation. The addition of this second gas flow at the level of the carina raises or lowers distal airway pressure, the magnitude of which is dependent on the direction and rate of gas flow. The beneficial effects of tracheal gas insufflation may be tempered by the long-term effects of altering distal airway pressure; lowering distal airway pressure may lead to atelectasis, whereas raising distal airway pressure may lead to an auto-positive end-expiratory pressure (auto-PEEP) effect.

Animals↗
PubMed1996

Sulindac and indomethacin inhibit formation of aberrant crypt foci in the colons of dimethyl hydrazine treated rats.

Aberrant crypt foci are microscopic lesions found in the colons of rodents treated with carcinogens, and in patients with premalignant colorectal conditions. They consist of single or multiple abnormal crypts and show cellular changes ranging from dysplasia to microscopic adenomacarcinoma. It is thought that these lesions represent the initial stages of the adenomacarcinoma path that results in the development of colorectal neoplasia. We have examined the effect of sulindac and indomethacin on the formation of aberrant crypt foci in rats treated with dimethylhydrazine (DMH). Aberrant crypt foci were induced in male Sprague-Dawley rats with two oral doses of dimethyl hydrazine at 25 mg/kg per dose. Rats were randomized to receive sulindac at 3 mg/kg (n = 20) or 10 mg/kg (n = 18) b.d., indomethacin at 1 mg/kg per day (n = 18) or 2 mg/kg per day (n = 19) or control (n = 37). Drug treatment was started on the day following the first dose of carcinogen and continued for 3 weeks. Colons were fixed flat overnight in 10% formalin and stained with 0.2%. Methylene Blue solution before being studied. There was a significant reduction in the number of aberrant crypt foci in rats treated with 10 mg/kg b.d., sulindac (P = 0.001) and indomethacin at 2 mg/kg per day (P = 0.0002). Sulindac, at 3 mg/kg b.d., and indomethacin, at 1 mg/kg per day, did not have a statistically significant effect (P = 0.089 and P = 0.052, respectively). None of the drug treatments affected the relative frequency of single crypt vs multiple crypt foci. Previous studies have shown that sulindac and indomethacin will significantly inhibit the growth and development of tumours in DMH treated rats. The current data suggest that one of the pathways of action of NSAID is to inhibit the formation of early preneoplastic lesions.

Animals↗
PubMed1996

Lactation performance of sows fed a bulky diet during gestation and receiving growth hormone-releasing factor during lactation.

Forty-eight crossbred gilts were used in an experiment with a 2 x 2 x 2 factorial arrangement of treatments. Treatments were control or bulky diet during gestation (4.1 vs 15.3% crude fiber), number of meals served daily in lactation (2 vs 4), and three daily s.c. injections of saline or of 1 mg of a human growth hormone-releasing factor analog (GRF-AN) from d 3 to 29 of lactation. Weights of pigs were recorded weekly until weaning on d 33. On d 29 of lactation, milk yield was estimated and a milk sample was obtained the next day. Jugular blood samples were collected from the sows on d 3 and 28 of lactation. Feed and water intake of sows were recorded throughout the study. Feeding the bulky diet decreased water intake in gestation (P < .001) and increased feed intake in lactation (P < .03). The number of meals served daily had no effect on feed intake during lactation (P > .1). Injections of GRF-AN increased IGF-I concentrations in blood (P < .01) and in milk (P < .001) and decreased backfat thickness (P < .001) and feed intake (P < .001) of sows during lactation. Pigs from sows injected with GRF-AN weighed more on d 14 (P < .05) and had a tendency to weigh more on d 7 (P = .09) and d 21 (P = .06). The incidence of sows showing estrus within 10 d of weaning was reduced with exogenous GRF (P < .01). The use of exogenous GRF in lactating sows may therefore have beneficial effects on growth of the pigs, yet the optimal nutritional and hormonal conditions needed for GRF to exert its full potential still need to be determined.

Animals↗
PubMed1996

Serum concentrations of micronutrients, packed cell volume, and blood hemoglobin during the first two gestations and lactations of sows.

The objective of the present work was to describe the changes in serum concentrations of some micronutrients during the first 2 gestations and lactations of 33 gilts in order to establish blood reference values for a rapid assessment of nutritional status. In both parities, blood samples were taken from the jugular vein at mating, 5, 10 and 15 wk of gestation and l d and 4 wk after parturition (weaning). Reference values (mean, standard deviation, minimum, maximum) for serum folates, vitamin B12, vitamin B6 metabolites (pyridoxal and pyridoxal-5-phosphate), calcium, phosphorus, sodium, zinc, copper and iron, as well as blood hemoglobin and packed cell volume are reported for each studied time. Differences between parities and between each time are also reported. Results from the present report demonstrate that knowledge of the physiological state of the sows is critical for the assessment of nutritional status of an individual or a breeding herd by interpretation of analyses of blood constituents.

Animals↗
PubMed1995

Mycoses of the breast: diagnosis by fine-needle aspiration.

Fungal infections of the breast are unusual and may clinically mimic carcinoma. When studied by fine-needle aspiration (FNA), such masses may yield necrosis, granulomatous inflammation, reactive histiocytes, and atypical epithelial cells. Cohesive groups of atypical epithelial cells featured nuclear enlargement and overlapping, as well as prominent nucleoli. The organisms may be widely scattered, so that careful evaluation was required for their identification. In concert with provocative clinical findings, these features may lead to an erroneous diagnosis of malignancy. We describe three women with mycotic masses of the breast initially studied by FNA. The first patient presented at age 31 with a large, firm breast mass, chest wall extension, and radiographic evidence of vertebral bone involvement. FNA was requested to confirm the clinical diagnosis of advanced breast carcinoma. In addition to the atypia described above, the smears showed yeast forms indicative of blastomycosis surrounded by neutrophils. She remains well, following antifungal treatment. The second case of Blastomycosis was diagnosed by FNA of a breast mass in a 64-yr-old woman, who also responded to treatment. The third patient's preoperative needle aspiration showed granulomas, but no organisms were identified, even with special stains; silver stains of surgically excised tissue showed histoplasmosis.

Adult↗
PubMed1994

Effect of bulky diets based on wheat bran or oat hulls on reproductive performance of sows during their first two parities.

The aim of the present study was to determine the effect of different degrees of bulkiness in the gestation diet on reproductive performance of sows during their first two parities. The density of the diets was modified by incorporating a high proportion of fibrous ingredients (wheat bran and corn cobs in Diet 2 and oat hulls and oats in Diet 3). A diet without fibrous ingredients was used as a control (corn and soybean meal in Diet 1). Although all the gestation diets were formulated to provide the same daily intake of the major nutrients, sows fed Diet 3 were the lightest and had the lowest backfat thickness (P < or = .05) during both parities, and those fed Diet 2 were the heaviest and the fattest (P < or = .05) during the second parity. These results seem to indicate an overestimation of the calculated nutritive value of Diet 3, whereas those obtained with Diet 2 seemed to be linked to its marked effect on behavior of sows during the second parity. There was no marked effect (P > .11) of treatments on the number of pigs born alive, on preweaning mortality, or on the weaning-to-estrus interval. At parturition, total litter weight was similar among treatments in the first parity, whereas in the second parity it was greater (P < or = .05) in sows fed Diet 2 than in those fed Diets 1 and 3. In both parities, feed intake of the common lactation diet given during lactation was 5% higher (P < or = .05) for sows fed Diet 3 during gestation than for sows fed the other diets.(ABSTRACT TRUNCATED AT 250 WORDS)

Adipose Tissue↗
PubMed1993

Aerobic versus strength training for risk factor intervention in middle-aged men at high risk for coronary heart disease.

To compare the effects of strength training (ST) to those of aerobic training (AT) for coronary heart disease (CHD) risk factor intervention, we studied 37 previously untrained males (aged 50 +/- 9 years, mean +/- SD) before and after 20 weeks of either ST (N = 14), AT (walk/jog, N = 13), or no exercise (inactive controls, N = 10). Lipoprotein and lipid profiles, blood pressure, and glucose and insulin responses to an oral glucose tolerance test (OGTT) were assessed before and after the training period in all three groups. The ST program produced significant reductions in plasma glucose levels at 60, 90, and 120 minutes (P < .05) after glucose ingestion, whereas the AT program resulted in significant reductions only at 90 and 120 minutes (P < .05). ST also decreased insulin levels during fasting (P < .05) and at 90 and 120 minutes (P < .01) after glucose ingestion. AT decreased insulin levels at 90 and 120 minutes (P < .01) after glucose ingestion. Both training programs reduced the total area under the glucose tolerance curve for glucose (both P < .05) and insulin (both P < .05), but there were no significant differences in these changes between the two groups. None of the glucose or insulin values were significantly altered in the control group. There were no significant changes in lipoprotein and lipid profiles or blood pressure in any of the three groups. These results suggest that ST and AT have comparable effects on risk factors for CHD.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1993

Age-related changes in secretion rate and post-secretory metabolism of growth hormone in swine.

The effect of age on growth hormone (GH) metabolism and GH-releasing factor (GRF)-induced GH concentrations were studied in 7 young (3 mo, 39 kg) and 7 old (30 mo, 156 kg) Yorkshire x Landrace female pigs. Jugular catheters were surgically inserted and 60 hr later total serum volume was determined. The following day, all animals were infused for 3 hr with GH (30.3 ng.min/kg B.W.) in order to calculate GH metabolic clearance rate (MCR), secretion rate (SR) and half-life (t 1/2). Two days later, 15 micrograms/kg of GRF was injected i.v. into all pigs. On a per animal basis, aging increased (P < .01) MCR (299 vs 132 ml/min), SR (714 vs 422 ng/min) and serum volume (6.6 vs 2.01), whereas t1/2 was unaltered (P > .1). Basal GH concentrations were lower in older pigs (P < .10) but the GRF-induced GH concentrations (measured as GH peak or area under the curve, AUC) were not affected by age (P > .1). Yet, when induced total GH secretion (AUC x MCR) and average total serum GH (mean GH post-injection x serum volume) were calculated per pig, these variables significantly increased between 3 and 30 mo of age. Basal IGF-I concentrations were lower in older pigs (P < .01), yet, there was a tendency (P = .10) for these pigs to show a greater IGF-I response to GH infusion. The present data therefore indicate that age alters both SR and post-secretory metabolism of GH.(ABSTRACT TRUNCATED AT 250 WORDS)

Aging↗
PubMed1993

Escape from in vivo restriction of Moloney mink cell focus-inducing viruses driven by the Mo+PyF101 long terminal repeat (LTR) by LTR alterations.

Mo+PyF101 M-MuLV is a variant Moloney murine leukemia virus containing polyomavirus F101 enhancers inserted just downstream from the M-MuLV enhancers in the long terminal repeat (LTR). The protein coding sequences for this virus are identical to those of M-MuLV. Mo+PyF101 M-MuLV induces T-cell disease with a much lower incidence and longer latency than wild-type M-MuLV. We have previously shown that Mo+PyF101 M-MuLV is defective in preleukemic events induced by wild-type M-MuLV, including splenic hematopoietic hyperplasia, bone marrow depletion, and generation of recombinant mink cell focus-inducing viruses (MCFs). We also showed that an M-MCF virus driven by the Mo+PyF101 LTR is infectious in vitro but does not propagate in mice. However, in these experiments, when a pseudotypic mixture of Mo+PyF101 M-MuLV and Mo+PyF101 MCF was inoculated into newborn NIH Swiss mice, they died of T-cell leukemia at times almost equivalent to those induced by wild-type M-MuLV. Tumor DNAs from Mo+PyF101 M-MuLV-Mo+PyF101 MCF-inoculated mice were examined by Southern blot analysis. The predominant forms of Mo+PyF101 MCF proviruses in these tumors contained added sequences in the U3 region of the LTR. The U3 regions of representative tumor-derived variant Mo+PyF101 MCFs were cloned by polymerase chain reaction amplification, and sequencing indicated that they had acquired an additional copy of the M-MuLV 75-bp tandem repeat in the enhancer region. NIH 3T3 cell lines infected with altered viruses were obtained from representative Mo+PyF101 M-MuLV-Mo+PyF101 MCF-induced tumors, and mice were inoculated with the recovered viruses. Leukemogenicity was approximately equivalent to that in the original Mo+PyF101 M-MuLV-Mo+PyF101 MCF viral stock. Southern blot analysis on the resulting tumors now predominantly revealed loss of the polyomavirus sequences. These results suggest that the suppressive effects of the PyF101 sequences on M-MuLV-induced disease and potentially on MCF propagation were overcome in two ways: by triplication of the M-MuLV direct repeats and by loss of the polyomavirus sequences.

3T3 Cells↗
PubMed1993

Digestive enzyme development in newborn piglets born of sows immunized against somatostatin and/or receiving growth hormone-releasing factor during gestation.

Thirty-eight second parity sows were either immunized (IMM) against somatostatin (SRIF) and/or injected with growth hormone-releasing factor (GRF) during gestation. Treatment effects on pancreatic, gastric and duodenal development as well as on digestive enzyme activity of piglets at birth (before suckling) or at 24 h postpartum were investigated. Birth weights of piglets were similar across treatments (p > 0.1). Weight, DNA, RNA, total protein content and enzyme activity for all three organs increased between birth and 24 h postpartum (p < 0.01), except for pancreatic RNA and chymotrypsin which decreased (p < 0.01), and protein content of the pancreas which was unaltered (p > 0.1). Gastric RNA, pancreatic weight:DNA, RNA:DNA and amylase:DNA ratios were increased in 1-day-old piglets from SRIF-IMM sows (p < or = 0.05). GRF only had significant effects (p < 0.05) on the maltase:DNA ratio, which it decreased. Yet, there were tendencies (p < 0.1) for duodenal weight, DNA and total protein content to be increased in piglets from GRF-injected dams. It is therefore apparent that major developmental changes of the pancreas, stomach and duodenum of piglets take place during the first 24 h postpartum. Injections of GRF and/or immunization against SRIF during gestation in swine also have several effects on digestive enzyme activity of neonatal pigs. Yet, the physiological implications of these early changes are not clear at the present time.

Animals↗