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Biomedical subjects

C F Shield

Publications and source records attributed to C F Shield.

87 records · Page 5Linked to original sources
PubMed1983

Stability of human lymphocyte differentiation antigens when stored at room temperature.

The development of monoclonal antibodies to human differentiation antigens and cytofluorographs have added a new dimension to immune monitoring. However, the technology is such that regionalization will most likely occur. We have demonstrated that human lymphocyte antigens OKT3, OKT4, OKT8 and OKT11 are stable in blood samples stored in ACD at room temperature for less than 72 h. This will allow samples to be analyzed in regional centers.

Antibodies, Monoclonal↗
PubMed1981

Humoral immunity in renal transplantation Detection of cytotoxic antibodies to lymphocyte antigens other than HLA-A,B,C and Ia-like antigens in patients with vascular graft rejection.

By using a Fab2 fragment blocking assay with cultured human B lymphoid cells and peripheral lymphocytes as targets, cytotoxic antibodies to Ia-like antigens, to HLA-A,B,C antigens, and to lymphocyte structures other than these two types of histocompatibility antigens were identified in serial post-transplant sera from 18 renal allograft recipients. Multiple combinations of these three types of antibodies were seen in 78% of the sera. A higher reactivity as well as higher titers of antibodies were found in patients with graft failure from rejection. The occurrence of anti HLA-A,B,C or anti Ia-like antibodies did not show any relationship to graft outcome. However, cytotoxic antibodies to other antigens (referred to as non-HLA antibodies) were detected in five of the six patients with graft failure and in only one patient with graft survival. In this latter patient, non-HLA antibodies occurred with a chronic rejection episode and were no longer detectable when the rejection ceased. This study shows that the Fab2 fragment blocking assay is a useful method to identify antibodies to multiple specificities in sera from kidney graft recipients, and suggests that non-HLA cytotoxic antibodies may be associated with graft rejection.

Antibody Formation↗
PubMed1979

Safe, cost-effective postoperative nutrition. Defined formula diet via needle-catheter jejunostomy.

To assess the safety and cost-effectiveness of needle-catheter jejunostomy for the purpose of feeding defined formula diet, we studied 199 consecutive patients who underwent major elective and emergency abdominal operations between July 1975 and June 1978 and in whom a needle-catheter jejunostomy was inserted. The complication rate was 2.5 per cent (1 per cent major and 1.5 per cent minor) during 7,238 patient-days of catheter exposure during which over 6 million calories were administered. There were no catheter-related deaths, bowel obstructions, bowel perforations, or intraperitoneal administration of feeding formula. The presence of a route of intestinal access and the use of defined formula diet in 111 patients who were unable to eat for over 10 days postoperatively resulted in a gross cost savings of almost $50,000 and a net savings of almost $33,000 by avoiding the necessity for central total parenteral nutrition. We belive the technique of needle-catheter jejunostomy is both safe and cost-effective in the administration of defined formula diet in the postoperative period, and we suggest that other surgeons gain experience with the technique to define its role in their own therapeutic armamentarium.

Adolescent↗
PubMed1976

The VIPoma: further confirmation of VIP as the hormonal agent in the WDHA syndrome.

The WDHA syndrome characterized by watery diarrhea, hypokalemia, and achlorhydria is being diagnosed with increasing frequency. The diagnosis has been made to date only due to severe clinical symptomatology. In a review of the literature gastrin, secretin, glucagon, enteroglucagon, gastric inhibitory peptide (GIP), vasoactive intestinal peptide (VIP), and prostaglandins have been variously suggested as a possible etiologic agent for this syndrome. A case of the WDHA syndrome is reported in which hormonal assays of the serum preoperatively and two years postoperatively and tumor for many of the proposed agents is performed. A discussion of possible cross-reactivity among these similary structured polypeptides in the radioimmunoassays systems is used to explain the multitude of possible hormonal agents presented in the literature. Standardization of the VIP assays will result in increasing diagnosis of this diseases state prior to its fulminant clinical presentation.

Achlorhydria↗
PubMed1975

Pseudoaneurysm of the brachiocephalic arteries: a complication of percutaneous internal jugular vein catheterization.

Three cases of arterial pseudoaneurysm formation as a complication of percutaneous internal jugular vein catheterization are presented. Laceration of the posterior common carotid artery or thyrocervical trunk was the source for these lesions. The common antecedent event was the attempted cannulation of the internal jugular vein with the lateral approach. A transcervical rather than transthoracic approach for treatment of these lesions was selected because of its low morbidity rate. This report documents another complication of internal jugular vein cannulation and recommends methods for prevention and successful treatment should it occur.

Adult↗