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Biomedical subjects

C F Fox

Publications and source records attributed to C F Fox.

At least 91 records · Page 5Linked to original sources
PubMed1974

Selective inhibition of Newcastle disease virus-induced glycoprotein synthesis by D-glucosamine hydrochloride.

d-Glucosamine selectively affected the synthesis of virus-induced glycoproteins in Newcastle disease virus-infected chick fibroblasts, but had no effect upon post-translational cleavage of the glycoprotein precursor for one of the two major glycoproteins of the mature virion. In response to glucosamine, at least one of the two virus-induced glycoproteins is formed in reduced quantity and the second is either not formed or has an electrophoretic mobility identical to that of another virus-induced protein.

Animals↗
PubMed1973

Manipulation of fatty acid composition in animal cells grown in culture.

The fatty acid composition of animal cells cultured in serum-free medium can be manipulated when the synthesis of endogenous fatty acids is inhibited by a biotin analog and fatty acids are supplied in the medium as detergent esters of Tween. When mouse LM cells were grown in medium supplemented with Tween-19:0 (an ester of Tween and nonadecanoic acid), odd chain fatty acid content of cellular phospholipids and neutral lipids increased from 1% to 75%. Concurrently, the saturated fatty acid content increased from 27% to 85%. Similar alterations in fatty acid content have been observed when BHK(21) cells are subjected to the same enrichment regime. The ability to control the fatty acid composition of cultured animal cells is a prerequisite to investigations into the role of the membrane lipid physical state in processes unique to these cells.

Animals↗
PubMed1973

Lateral phase separations in membrane lipids and the mechanism of sugar transport in Escherichia coli.

Changes in slope of Arrhenius plots for transport can, in some instances, be detected at two different temperatures for cells that have a relatively simple fatty-acid composition in the membrane lipids. These characteristic temperatures correlate with the characteristic temperatures that define changes of state in membrane phospholipids as revealed by the paramagnetic resonance of the spin label TEMPO (2,2,6,6-tetramethylpiperidine-1-oxyl). The higher of these characteristic temperatures is that at which the formation of solid patches of membrane lipids is first detected. The lower is the end point of the course of lateral phase separations, at which all the membrane lipids are in a solid phase. For cells enriched for elaidic acid, the rate of transport increase by as much as 2-fold as the temperature is decreased by less than 1 degrees , at the higher characteristic temperature. At this characteristic temperature, lateral phase separations begin in the membrane phospholipids. This is also the temperature where one predicts a striking increase in the lateral compressibility of the membrane lipids. These data are thus interpreted to indicate that a component of the transport system vertically penetrates one or both monolayer faces of the membrane during transport, or that some other event involving the lateral compression of the phospholipids is important for transport.

Biological Transport, Active↗
PubMed1973

Precursor protein for Newcastle disease virus.

The course of viral protein synthesis during infection of chicken embryo fibroblasts with Newcastle disease virus (NDV) L. Kansas has been followed by using sodium dodecyl sulfate polyacrylamide gel electrophoresis. Of the three major virion polypeptide molecular weight classes, I (78,400 daltons), II (53,500 daltons), and III (37,600 daltons), only II, having the same electrophoretic mobility as nucleocapsid polypeptide, appears to be the cleavage product of a precursor polypeptide PII (64,800 daltons) detected in NDV-infected cells after brief labeling with radioactive amino acids. Nucleocapsids were isolated from NDV-infected cells which had been pulse-labeled with radioactive amino acids or pulse-labeled and further incubated with unlabeled amino acids. Gel electrophoretic analysis of proteins derived from nucleocapsids showed that an increase in the period of incubation with unlabeled amino acids resulted in an increase in the amount of radioactivity in nucleocapsid protein. Polypeptide PII was not detected as a transient component of the isolated nucleocapsid fraction. These results are consistent with two interpretations. The product of PII cleavage is (i) nucleocapsid polypeptide, or (ii) a nonvirion or minor envelope polypeptide having the same electrophoretic mobility as nucleocapsid polypeptide.

Animals↗