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Biomedical subjects

C Eichler

Publications and source records attributed to C Eichler.

24 records · Page 2Linked to original sources
PubMed1986

[In vitro sensitivity of T lymphocytes to theophylline in healthy adults and patients following cadaver kidney allotransplantation].

In the peripheral blood of healthy adults the number of theophylline-resistant T-lymphocytes (T-res) is 51 +/- 4% and 1,161 +/- 326/microliter, respectively, and the number of theophyllins-sensitive 11 +/- 2% and 252 +/- 95/microliters. The membrane markers show a heterogeneous distribution. The quotient from T-res/T-sens is 4.9 +/- 1.3. Within the T-sens 14% are CD4+ and 30% CD8+, 29% carry Fc-IgC and 34% Fc-IgM-receptors. Thus the T-res in their netto-function were helper cells and the T-sens suppressor/cytotox cells. Before the transplantation the preoperative ratio of the two subpopulation is significantly diminished (Q = 3.66 +/- 1.53), however, prognostic statements cannot be deduced from this. Activations of the immune system (rejection crises, cytomegalovirus infections) are accompanied by significant diminutions of the T-sens, whereby the T-res/T-sens-Quotient increases. Thus they are unequivocally included into immunoregulatory processes.

Adolescent↗
PubMed1985

[incidence of cytomegalovirus infections following transplantation of kidneys from seropositive and seronegative donors].

Serum samples of 110 recipients of kidney transplants and the 100 donors belonging to them taken before the transplantation were examined for complement-binding antibodies against cytomegaloviruses. A clear influence of the cytomegalovirus antibody state of donor and recipient on the cytomegalovirus infection appearing after transplantation, serologically ascertained by cytomegalovirus-IgM-antibody proof or seroconversion and fourfold increase of the titre in the complement-binding reaction, respectively, could be made evident. Cytomegalovirus negative recipients of kidneys of cytomegalovirus positive donors had significantly more infections than seronegative recipients of kidneys of seronegative donors (50% vs. 16%, p less than 0.05). According to the infection rate the seropositive recipients stand with about 30% between these two groups. It seems that in these recipients the serological state of the donors has no influence. These findings make evident that seronegative recipients receive the cytomegaloviruses essentially with the transplant, whereas the cytomegalovirus infections in seropositive recipients are endogenous cytomegalovirus reactivations caused by therapy.

Adolescent↗
PubMed1985

[Frequency of infection with cytomegaloviruses (CMV) following kidney transplantation and initial experiences with CMV gamma globulin prevention].

In a retrospective study we investigated 364 patients who had received cadaveric kidney transplants between 1978 and 1984 as to cytomegalovirus (CMV) infections using complement-fixation test and indirect immunofluorescent test (IgM, IgG). Before transplantation, 194/364 patients were seropositive and 170/364 were seronegative. After transplantation, seroconversion or an increase in titre (greater than or equal to 4 fold) was found in 26% of seropositive recipients and in 30% of seronegative recipients. In contrast to the secondary infections (approximately 40% asymptomatic or oligosymptomatic), the primary infections were regularly connected with a clinical symptomatology. As to the recipient's and donor's pre-transplant antibody status the highest infection rate (62%) was seen in the group of seronegative recipients of kidneys from seropositive donors. Preformed CMV antibodies seem to be useful in preventing severe CMV-diseases. Therefore, in April 1985 we started a randomized study in order to investigate the efficacy of an i.v. cytomegalovirus-immunoglobulin (CMV-Polyglobin/Cutter). There were no side effects. Already 4 h after infusion we were able to detect CMV-IgG-antibodies (IFT) in peripheral blood. This passive immunization, however, was not capable to prevent a CMV infection in each case; there were 5/8 seroconversions and 4/8 symptomatic CMV infections. Nevertheless, we think the application of CMV immunoglobulin has a beneficial effect.

Antibodies, Viral↗
PubMed1985

[Serologic diagnosis of primary cytomegalovirus infections in patients following kidney transplantation using the indirect immunofluorescence technic and the complement-fixation test].

Sera of 22 recipients of kidney transplants with clinically and serologically (complement binding reaction) ascertained cytomegalovirus infection were retrospectively examined for virus-specific antibodies of the class IgM by means of the indirect immunofluorescence test. Already two to five days after the appearance of the adequate clinical symptoms (leucopenia, fever, T-lymphocytopenia, increase of creatinine and thrombocytopenia) CMV-IgM-antibodies could be proved. On average a positive complement binding reaction was found only two weeks later. In other 13 patients of the actual control programme the CMV-IgM-antibody findings first obtained were compared with the results of the complement fixing reaction obtained later. Neither falsely positive nor falsely negative results could be found.

Antibodies, Viral↗
PubMed1978

[Detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)].

The macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (BGG) in guinea pigs. The test was performed with peritoneal cells (PC) in hemocytometer chambers. 10 mg/ml BGG were added as antigen. The washing step was performed with a burette. An average standard deviation of 5% was obtained with this washing method. Day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated immunity against BGG. Supernatants of sensitized spleen cells or lymph node cells produce adherence inhibition in PC of nonimmunized guinea pigs too.

Animals↗