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Biomedical subjects

C E Stager

Publications and source records attributed to C E Stager.

26 records · Page 2Linked to original sources
PubMed1981

Clinical laboratory evaluation of the automicrobic system Enterobacteriaceae biochemical card.

The AutoMicrobic System Enterobacteriaceae Biochemical Card (AMS-EBC; Vitek Systems, Inc.) was evaluated in two clinical microbiology laboratories. A total of 502 consecutive clinical isolates representing members of the family Enterobacteriaceae were tested in parallel with the AMS-EBC, API 20E, and Enterotube II systems. Discrepancies between systems were resolved with the conventional methods of Edwards and Ewing (P. R. Edwards and W. H. Ewing [ed.], Identification of Enterobacteriaceae, 1972) and Ewing and Martin (W. H. Ewing and W. J. Martin, in Manual of Clinical Microbiology, 1974) AMS-EBC correctly identified 96.6% and incorrectly identified 3.4% of the isolates. When 12 or more isolates of a species were evaluated, Serratia marcescens, Proteus mirabilis, and Enterobacter cloacae posed the greatest challenge to the systems, with 92.6, 95.2, and 95.3%, respectively, being correctly identified. To confirm the accuracy of identification when all systems agreed, 93 randomly selected isolates were identified by conventional methods. The percent agreement was 100%. The reproducibility of triplicate determinations on 93 randomly selected isolates with the AMS-EBC was 99.6%. The AMS-EBC was found to be an easy, rapid, and accurate method for identification of Enterobacteriaceae.

Bacteriological Techniques↗
PubMed1979

Evaluation of a modified dye pour-plate auxanographic method for the rapid identification of clinically significant yeasts. Comparison with two commercial systems.

A modified dye pour-plate auxanographic (DPPA) method for the presumptive identification of medically important yeasts was evaluated, in a comparative study with a conventional procedure, the API 20C clinical yeast system (Analytab Products Inc.), and the Uni-Yeast-Tek (UYT) system. The 174 coded clinical isolates were members of the genera Candida, Cryptococcus, Rhodotorula, Saccharomyces, Torulopsis, and Trichosporon. The identification accuracies with DPPA, API, and UYT were 95%, 93%, and 99% respectively. DPPA and API required more time to inoculate but gave rapid identification profiles. UYT was simple to inoculate and both UYT and DPPA were easy to read. Cost analysis of the three rapid methods demonstrated DPPA to be the most economical making it a feasible alternative for small clinical laboratories as well as large laboratories possessing the facilities to make their own media.

Carbohydrate Metabolism↗
PubMed1978

Comparison of several culture media used for studies on mycobacteriophages.

The value of RVA, N-1, 7H10, 7H11 and Sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. Experiments were made with mycobacteriophages BGI, BKI, CRI-3, G37, and LG, all of which lyse Mycobacterium smegmatis strain 607B, and with mycobacteriophage DS6A which lyses Mycobacterium tuberculosis strain H37Rv. The methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. It was found that N-1, 7H10 and 7H11 media gave better overall results than RVA medium for M. smegmatis strain 607B and its phages, and that RVA medium was generally the most useful for M. tuberculositems employed.

Culture Media↗
PubMed1978

Phage typing of mycobacteria using paper discs.

The suitability of phage-impregnated paper discs for the phage typing of mycobacteria was studied. The relevance of the routine test dilution, the volume of the phage used, the mode of incubation, and the effect of prolonged storage of phage-impregnated paper discs were considered. By using paper discs, each impregnated with one of 5 different mycobacteriophages (BG1, BK1, G37, CRI-3 and LG) that lyse Mycobacterium smegmatis 607B, it was determined that 100 x the routine test dilution in a volume of at least 20 microliter was required for phage lysis. Soaked and dried paper discs produced larger areas of lysis than those with 20-microliter volumes. Soaked discs were found to be stable even after storage for 8 weeks at 4 degrees C.

Bacteriological Techniques↗
PubMed1973

Differential medium for Vibrio cholerae.

A differential medium designed for rapid presumptive identification of Vibrio cholerae was described and shown to be useful for enumeration of viable cholera vibrios in the presence of other intestinal bacteria.

Cholera↗
PubMedDate not supplied

Evaluation of the FiltraCheck-UTI for detection of bacteriuria.

The FiltraCheck-UT1 (FC) is a disposable colorimetric urine screen for bacteriuria that requires less than 1 min to perform. The results of the FC and two other urine screens, the Bac-T-Screen (BTS) and the Chemstrip LN test strip (LN), were compared with quantitative culture method. A total of 551 urine specimens were tested. The sensitivity of FC, BTS, and LN for probable pathogens at greater than or equal to 10(5) CFU/ml was 94.8%, 97.4%, and 77.9% respectively. These values for specimens with probable pathogens at greater than or equal to 10(4) CFU/ml were 91.1%, 92.1%, and 74.3%, respectively. When the LN was combined with the FC or BTS as a urine screen, the sensitivity for probable pathogens was improved.

Bacteria↗
PubMedDate not supplied

Pulmonary coccidioidal pseudomycetoma.

Coccidiomycosis is rarely associated with a pulmonary mycetoma. We report a patient with progressive cavitary coccidiomycosis, whose initial radiographic and clinical appearance simulated a mycetoma. Examination of the surgically resected lung showed necrotizing Coccidioides immitis granulomas with spherules and arthroconidialike structures, but no evidence of a mycetoma. We propose the term pulmonary coccidioidal pseudomycetoma as the best descriptor for this patient's clinical, radiographic, pathologic, and microbiologic presentation.

Adult↗