Biomedical subjects

C E Price

Publications and source records attributed to C E Price.

36 records · Page 2Linked to original sources

PubMed1976

Metabolism of [4C]crufomate (4-t-butyl-2-chlorophenyl methyl methylphosphoramidate) by the sheep.

Twenty-five metabolites were isolated from the urine, feces or plasma of sheep given single oral doses of [14C]crufomate (4-t-butyl-2-chlorophenyl methyl methylphosphoramidate). These metabolites resulted from one or more of the following transformations: oxidatin of a methyl group in the t-butyl moiety to yield either an alcohol or a carboxylic acid; hydrolysis of the phosphate and phosphate and phosphoramidate bonds; oxidatin of the N-methyl group to yield N-formyl phosphoramidates; methylation of the N-formyl group to yield N-methyl-N-formyl phosphoramidates; oxidative N-demethylation; conjugation with glucuronic acid. No ring-hydroxylation of dechlorination was observed, and no crufonate was isolated from the urine, feces or plasma.

Animals↗

PubMed1976

Mass spectral characterization of the glucuronide conjugates of crufomate (4-t-butyl-2-chlorophenyl methyl methylphosphoramidate) metabolites.

Seven sheep metabolites of crufomate (4-t-butyl-2-chlorophenyl methyl methylphosphoramidate) were characterized as glucuronide conjugates by interpretation of mass spectral data obtained from ths of the methyl esters.

Amides↗

PubMed1976

Metabolism of O,O-dimethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate in sheep and rats and of 3,5,6-trichloro-2-pyridinol in sheep.

Sheep and rats metabolized single oral doses of O-O-dimethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate (I) to three major metabolites that were excreted in the urine (approximately 70% of the 14C). These were the glucuronide of 3,5,6-trichloro-2-pyridinol, O-methyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate, and 3,5,6-trichloro-2-pyridinol. The latter two metabolites and the parent compound were isolated from sheep feces. Sheep plasma contained the same metabolites that were found in sheep urine, and no parent compound was detected in the plasma. Tissue residues from I were determined. Visceral fat contained the highest concentration of I-equivalents (11.8 ppm). Sheep excreted a single oral dose of 3,5,6-trichoropyridinol (II) unchanged in the feces and as II-glucuronide in the urine.

Animals↗

PubMed1976

Rat urinary metabolites from O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl) phosphorothioate.

Rats metabolized single oral doses of O,O-diethyl-O(3,5,6-trichloro-2-pyridyl-2,6-14C) phosphorothioate to at least six radiolabeled urinary metabolites. The urine contained about 90 percent of the dose. Three of these metabolites were identified as the glucuronide of 3,5,6-trichloro-2-pyridinol (80% the urinary 14C), a glycoside of 3,5,6-trichloro-2-pyridinol (4%), and 3,5,6-trichloro-2-pyridinol (12%).

Animals↗

PubMed1969

Purine metabolism in germinating wheat embryos.

1. Both the acid-soluble fraction and the nucleic acid fraction of wheat embryos were extensively labelled after incubation for 6hr. in the presence of [8-(14)C]adenine. Subsequent incubation in the absence of labelled adenine resulted in no loss of radioactivity to the medium during a 48hr. period. Radioautography indicated that during this period there was a continuous increase in the radioactivity present in the acid-insoluble fractions of the root and leaf tissues relative to that present in the coleorhiza and coleoptile. 2. During incubation at 25 degrees there was a 26-fold increase in the activity of 3'-nucleotidase between 4hr. and 24hr.; the activities of enzymes hydrolysing AMP and IMP increased to a smaller extent. The activities of adenine phosphoribosyltransferase and hypoxanthine phosphoribosyltransferase increased three- to five-fold during incubation at 25 degrees for 24hr. 3. Adenosine kinase, inosine phosphorylase and 5-phosphoribosyl pyrophosphate synthetase activities were high in extracts from dry embryos and did not increase during 48hr. at 25 degrees . 4. The increase in 3'-nucleotidase activity was prevented by cycloheximide, cryptopleurine or incubation at 4 degrees , but not by actinomycin D; these treatments did not depress the activity of the other enzymes measured. 5. The results are discussed in relation to RNA translocation within the wheat embryo during germination.

Adenine↗

PubMed1969