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Biomedical subjects

C E Davis

Publications and source records attributed to C E Davis.

205 records · Page 12Linked to original sources

Frequency of early-morning rise in blood glucose in children with diabetes at camp.

To estimate the frequency of an early-morning glucose rise (EMR) in relatively unselected children with insulin-dependent diabetes mellitus (IDDM), we assessed capillary blood glucose (CBG) at midsleep (0200-0430) and prebreakfast (0700-0800) in 97 children with diabetes at camp. The EMR (prebreakfast CBG-midsleep CGB) was inversely related to the midsleep CBG level (r = -.45, P less than .001). Of the 49 children with midsleep CBG less than 200 mg/dl, the mean EMR was 34 +/- 60 mg/dl, and 18 of these children had rises of greater than 40 mg/dl. In conclusion, when midsleep glycemia is less than 200 mg/dl, a rise in blood glucose from midsleep to prebreakfast, often greater than 40 mg/dl, is a common element of glycemic control among children with IDDM. The relative importance of the Somogyi phenomenon, the dawn phenomenon, and mere insulin insufficiency in the early-morning hours cannot be determined from these data.

Adolescent↗

[The transmission of maternal measles antibodies to newborn infants in the Ivory Coast].

Authors studied the disappearance of mothers measles antibodies among 286 babies from Ivory Coast 250 of which were living in Adzopé, a town with 30,000 inhabitants at 100 km up North from Abidjan and the 36 other babies in Abidjan. The study of antibodies by IHA showed a higher serum incidence rate of mother antibodies among 4 months aged babies in Abidjan. The more sensitive study of antibodies by the Mann serum neutralisation, shows that when 6 months aged, 60% babies from Adzope still have antibodies and when 10 months aged, none of the babies is positive. Serum negativation is late compared to Brazzaville when antibodies level is lower.

Age Factors↗

[Epidemiological aspects of meningitis in the north of the Ivory Coast].

Classically, the North of Ivory Coast is not part of the "meningitis belt". However, recent health statistics put in light some recent outbreaks in this part of the country, the last one in the years 1983-1985. The authors have studied this last outbreak and it can be foreseen that these epidemics will appear again as in the more Northern countries. Therefore it is desirable to set up a monitoring system to better organize the fight against this disease.

Adolescent↗

Effect of pH on absorbance of azo dye formed by reaction between nitrite and sulfanilamide/N-(1-naphthyl)ethylenediamine in residual nitrite methods for foods.

Current methods used for determining residual nitrite concentration in foods involve forming an azo dye that is measured spectrophotometrically. Conventional procedures do not specify control of pH for the final colored solution. Because many indicator dyes are pH-dependent, absorbance of the azo dye may vary not only because of nitrite concentration differences, but also because of pH variation. Thus, erroneous results could arise from a standard curve developed at one pH and used for a test sample at a different pH. This would cause an inaccurate concentration conversion from the standard curve. In this study, absorbances at lambda max wavelengths were recorded at 28 pH values (range 1.0-4.5) for each of 5 nitrite concentrations. A mathematical equation was fitted to these data and its plot gives a 3-dimensional response surface showing the relationships between pH, nitrite concentration, and absorbance. A modification for the spectrophotometric measurement of nitrite is proposed.

Azo Compounds↗

Fluorometric determination of acid phosphatase in cooked, boneless, nonbreaded broiler breast and thigh meat.

This method is applicable for determining activity of acid phosphatase (ACP), a heat-labile enzyme, in cooked, boneless, nonbreaded broiler marinated (83.65% meat) and nonmarinated (100% meat) breast and thigh and in a 50:50 blend of breast and thigh meat. The assay uses a self-indicating substrate that, when acted upon by ACP, loses a phosphate radical and becomes a highly fluorescent compound. Cooked meat is added to deionized distilled water in a 1:3 ratio, blended with a hand-held homogenizer, and then centrifuged at 2500 relative centrifugal force for 5 min. ACP activity in the filtrate is measured after shaking on a Vortex mixer 75 microL of the extract with a pH 5.00 acetate buffer containing a nonfluorescent aromatic monophosphoric ester substrate. The rate of fluorophore formation is monitored during a 3 min incubation period (38 degrees C) in a fluorometer, and ACP enzyme activity (mU/kg sample) is calculated. Three laboratories analyzed 6 cooked poultry products (marinated and nonmarinated breast, thigh, and 50:50 breast/thigh blend). Five cooking temperatures were used to generate different ACP activity levels, which were replicated twice with duplicate samples and duplicate sample tests representing 720 data points. Log10 ACP activity (mU/kg sample) performance repeatability and reproducibility standard deviations (sr and sR) and relative standard deviations (RSDr and RSDR) over 5 cooking treatments for 6 products were as follows: marinated breast: sr = 0.02, sR = 0.08, RSDr = 0.60%, RSDR = 2.12%; nonmarinated breast: sr = 0.02, sR = 0.04, RSDr = 0.66%, RSDR = 1.29%; marinated thigh: sr = 0.01, = 0.01, RSDr = 0.37%, RSDR = 0.37%; nonmarinated thigh: sr = 0.02, sR = 0.05, RSDr = 0.53%, RSDR = 1.43%; marinated 50:50 breast/thigh blend: sr = 0.01, sR = 0.05, RSDr = 0.36%, RSDR = 1.31%; nonmarinated 50:50 breast/thigh blend: sr = 0.01, sR = 0.04, RSDr = 0.32%, RSDR = 1.12%.

Acid Phosphatase↗