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Biomedical subjects

C Doglioni

Publications and source records attributed to C Doglioni.

At least 127 records · Page 7Linked to original sources
PubMed1991

Immunohistochemical evidence of abnormal expression of the antioncogene-encoded p53 phosphoprotein in Hodgkin's disease and CD30+ anaplastic lymphomas.

The gene encoding p53 phosphoprotein, originally believed to be an oncogene, recently has been proposed as a candidate antioncogene (tumor-suppressor gene). Abnormalities of the p53 gene expression have been demonstrated in different human malignancies including carcinomas and sarcomas, but little information concerning p53 immunoreactivity in human lymphomas is so far available. In this study immunohistochemical staining for p53-protein was performed on frozen- and paraffin-embedded samples from patients with Hodgkin's (HD) and non-Hodgkin's lymphomas (NHL). No p53 immunoreactivity could be demonstrated in any cell type in nonneoplastic lymphoid samples, including germinal center cells in reactive lymph nodes and cortical thymocytes. On the other hand, a significant proportion of p53+ neoplastic cells was observed in 23 of 31 cases of HD and 17 of 68 cases of NHL. All positive lymphoma cases were diagnosed as high-grade or CD30+ anaplastic NHL. The demonstration of abnormal expression of p53 protein in these diseases can contribute to addressing unresolved issues regarding the origin and pathogenesis of HD and CD30+ anaplastic lymphomas.

Antibodies, Monoclonal↗
PubMed1990

Cytokeratin-immunoreactive cells of human lymph nodes and spleen in normal and pathological conditions. An immunocytochemical study.

The occurrence and the distribution of cytokeratin (CK)-immunoreactive reticulum cells in a series of normal and pathological human lymph nodes and spleens are documented. The immunoreactive cells exhibit morphological and immunophenotypic features of so-called fibroblastic reticulum cells, with or without myoid differentiation. They invariably co-express vimentin and, to a lesser extent, desmin and muscle-specific actin isoforms. These CK-immunoreactive cells are apparently a normal subpopulation of reticulum cells, being detectable from the early stages of spleen and lymph node development. They are distributed mainly in the paracortical and medullary regions of the lymph nodes and at the periphery of the white pulp in the spleen. Their number and distribution are highly variable in different neoplastic and non-neoplastic pathological conditions but the changes are not disease specific. CK-immunoreactive reticulum cells are easily identifiable in both frozen and fixed lymphoid tissue and in cytological smears of fine-needle aspirates, provided that monoclonal antibodies whose spectrum of reactivity includes cytokeratins 8 and 18 are used. The awareness of the occurrence of CK-immunoreactive cells in normal lymphoid tissues is of particular relevance in the search for micrometastatic foci using anti-CK antibodies.

Adult↗
PubMed1990

Immunocytochemical localization of progesterone receptors in endocrine cells of the human pancreas.

Progesterone receptors (PgR) have been immunocytochemically localized in the nuclei of several (40% to 75%) endocrine cells of the human pancreas and in a more variable number of neoplastic cells of 7 of 18 endocrine pancreatic tumors. Conversely the exocrine epithelial cells of the pancreas did not exhibit any PgR immunoreactivity in normal as well as in different pathologic conditions, including pancreatic adenocarcinomas. Estrogen receptors were not detected in any of the pancreatic samples investigated. Double immunocytochemical experiments have documented that PgR immunoreactivity in normal Langerhans islets is a consistent feature of most (75%) glucagon-producing A cells, of approximately 5% to 20% of insulin-producing B cells, and of a variable percentage of pancreatic polypeptide (PP)-producing cells, ranging from 5% to 70%. These figures were not affected by the sex, age, or underlying disease of the patients. The reported findings corroborate previous clinical and experimental evidence indicating that sex steroid hormones may have some regulatory effects on the functional activity of the endocrine pancreas.

Adolescent↗
PubMed1988

Infantile digital fibromatosis-like tumour (inclusion body fibromatosis) of adulthood: report of two cases with ultrastructural and immunocytochemical findings.

Two adult cases of a cutaneous lesion indistinguishable from typical infantile digital fibromatosis are added to the unique similar case so far reported in adulthood. The immunocytochemical localization of vimentin and muscle actin in the proliferating cells confirms their myofibroblastic nature and establishes closer relationships between the adult and the infantile variants of this entity. These two variants, however, appear to be clinically different, since all the adult cases were extradigital and did not recur after surgical excision. The term inclusion body fibromatosis underlines the histological hallmark of the lesion and should be used to identify this entity in place of recurrent infantile digital fibromatosis which does not seem any longer appropriate.

Actins↗
PubMed1988

Glial fibrillary acidic protein immunoreactivity in human respiratory tract cartilages and pulmonary chondromatous hamartomas.

Immunocytochemical investigation was performed on a large series of adult, neonatal, and fetal respiratory tract cartilages to ascertain their immunoreactivity for glial fibrillary acidic protein (GFAP) and vimentin. Two polyclonal and six different monoclonal antibodies were used to document the presence of GFAP-immunoreactive chondrocytes in all the fetal and neonatal cartilages as well as in the adult epiglottis, arythenoids, and lobar, segmental, and subsegmental bronchi. The number of chondrocytes showing GFAP immunoreactivity decreased from fetal life to adulthood. Simultaneous immunoreactivity for GFAP and vimentin has also been ascertained in chondrocytes and in perichondrial stellate or elongated cells of the 25 chondromatous hamartomas investigated. These findings document yet another "inappropriate" pattern of intermediate filament immunoreactivity in normal and neoplastic human cells, and contradict the widely held supposition that the expression of GFAP is restricted to cells of glial origin.

Adult↗
PubMed1988

Ependymoma of the mediastinum.

A case of primary ependymoma of the mediastinum is reported. The tumor was adherent to the lung and metastasized to adjacent mediastinal lymph nodes. An autopsy showed no evidence of tumor in the central nervous system. The diagnosis of ependymoma was confirmed by the immunohistochemical positivity for glialfibrillary acidic protein. To the best of our knowledge, this is the first reported example of an ependymoma in this location.

Ependymoma↗
PubMed1987

Growth fraction in human brain tumors defined by the monoclonal antibody Ki-67.

The monoclonal antibody Ki-67, which reacts with cells in the active part of the cell cycle, was used to evaluate immunocytochemically the growth fraction in 22 primary brain neoplasms. The percentage of labelled cells reflected the histological grade of malignancy of each neoplasms. High percentage of Ki-67-positive cells were observed in one choroid plexus carcinoma (60%), one primary melanoma of meninges (40%), three medulloblastomas (40%-50%), one anaplastic astrocytoma and six glioblastomas (10%-40%). One ependymoma had 7% positive cells. Rare positive cells (1%) were present in one pilocytic astrocytoma and one ganglioglioma. Except one negative case, the meningiomas (five cases) had values of positivity ranging from 1% to 6%. Two acoustic schwannomas were negative. These results suggest that immunocytochemical staining with the Ki-67 may be a useful method for measuring the growth fraction in brain neoplasms.

Antibodies, Monoclonal↗
PubMed1987

Choroid plexus tumors. An immunocytochemical study with particular reference to the coexpression of intermediate filament proteins.

Sixteen choroid plexus tumors (CPTs) have been investigated for the localization of different immunocytochemical markers of epithelial and nonepithelial nature, namely, simple epithelial-type cytokeratins, vimentin, glial fibrillary acidic protein (GFAP), a panepithelial antigen defined by the lu-5 monoclonal antibody (lu-5 antigen), S-100 protein, and epithelial membrane antigen (EMA). Intermediate filament proteins have been identified in paraffin sections of 14 of 16 cases (87.5%). In all these tumors, cytokeratins and vimentin were constantly coexpressed by the neoplastic cells, in a manner similar to that of the cells lining normal choroid plexus. In 7 of these 14 cases, in addition to cytokeratins and vimentin, the neoplastic cells were shown to coexpress GFAP, which is not synthesized by their normal cell counterpart. The appearance of GFAP immunoreactivity in CPTs might be related to an ependymal differentiation of the neoplastic cells, because normal ependyma and ependymomas constantly coexpress GFAP and vimentin. The simultaneous expression of three distinct intermediate filament proteins by the same neoplastic cells is an exceedingly rare phenomenon, which has never been reported by double labeling technique in neoplasms of the central nervous system. Despite the complex antigenic profile of the CPT, which includes immunoreactivity for lu-5 antigen, S-100 protein, and EMA in most of the cases, positivity for three different epithelial markers indicates that these tumors have an epithelial nature. Moreover, the immunocytochemical typing of CPT with the panel of antibodies used in the current investigation allows differentiation from other primary and metastatic central nervous system tumors.

Antibodies, Monoclonal↗
PubMed1987

Secondary amyloidosis and cystic fibrosis. A morphological and histochemical study of five cases.

The pathological findings of five cases of amyloidosis associated with Cystic Fibrosis are reported. Rectal biopsy led to the diagnosis of amyloidosis in four patients. In three cases the diagnosis was confirmed at autopsy, with involvement of spleen, liver, kidneys, adrenal glands, thyroid and other organs. It seems that Secondary Amyloidosis provokes a significant, although rare, complication of Cystic Fibrosis as greater numbers of these patients survive into adulthood.

Adult↗
PubMed1986

Mediastinal large-cell lymphoma of B-type, with sclerosis: histopathological and immunohistochemical study of eight cases.

Eight cases of mediastinal non-lymphoblastic large-cell lymphoma have been studied by histopathological and immunohistochemical methods. A common clinical, morphological and immunophenotypic pattern was identified. Six of eight cases proved to be of B-cell origin by the expression of B-associated antigens detected by specific monoclonal antibodies. Cells of large size with nuclei of varying morphology and a peculiar type of fine compartmentalizing fibrosis were observed in all specimens. Clinically the disease was characterized by the young age of the patients, primary mediastinal involvement, aggressive behaviour and spread to unusual sites (kidneys in four cases). This seems to be a hitherto unrecognized entity in the field of non-Hodgkin's lymphomas, often misdiagnosed because of location and a morphology uncommon for B-cell malignancies. Immunohistochemical analysis on frozen tissue sections appears to be mandatory for a correct diagnosis. Nevertheless, this type of lymphoma could be suspected also on the basis of its peculiar clinicopathological characteristics.

Adolescent↗
PubMed1984

Metachronous coexistence of laryngeal pseudolymphoma and squamous cell carcinoma. An unreported case.

An unreported case of metachronous coexistence of laryngeal pseudolymphoma and squamous cell carcinoma is described. The long follow-up of the patient (above 11 years), without any evidence of lymphoreticular disease elsewhere, was in favor of a diagnosis of lymphoid hyperplasia of the larynx. The occurrence in the same organ of a squamous cell carcinoma has allowed the thorough investigation of the reactive lesion. The additional cases of laryngeal pseudolymphoma reported in the English literature are reviewed.

Aged↗
PubMed1984

[Echographic study of the structure of a malignant melanoma of the choroid].

The case of a 74 yr. old male affected by a mixed cell choroidal melanoma is reported. Ophthalmoscopic examination suggested a choroidal melanoma; this diagnosis was confirmed by fluorescein angiography, ultrasonography and CT-scan. In particular, contact ultrasonography (A-mode and B-mode), following topographic, kinetic and quantitative diagnostic criteria, revealed a solid lesion with the typical features of a choroidal melanoma. Ultrasonography by differential selective echographic technique revealed that the lesion consisted of two components with different acoustic features : the first characterized by a 41 dB reflectivity (peripheral and superior portions), the second by a 23 dB reflectivity (central and inferior portions). Attenuation values were 1.2 dB/mm and 0.7 dB/mm, respectively. CT-scan did not show any significant change in density within the mass. Histopathologic evaluation confirmed a clear-cut edge between the epitheloid and the spindle-cell components. The more reflecting lesion (23 dB), located in the central-inferior portion of the lesion, was histologically less dense and was due to the epitheloid component. The superiorally located more attenuating lesion (1.2 dB/mm) was histologically more dense and due to the spindle-cell component.

Aged↗
PubMed1983

Sclerosing stromal tumor of the ovary. A hormonal, histochemical and ultrastructural study.

Three new cases of sclerosing stromal tumor of the ovary have been studied by hormonal, immunohistochemical and electron microscopic analysis. The results confirm that this tumor may have hormonal activity. Ultrastructural study shows findings different from those previously reported. The authors propose an origin from the ovarian stroma with luteinization of stromal tumor cells.

Adolescent↗
PubMedDate not supplied

Gastric non-Hodgkin's lymphoma: analysis of 252 patients from a multicenter study.

AIMS AND BACKGROUND: The stomach is the most common site of primary extranodal non-Hodgkin's lymphoma (NHL) and no agreement has been reached so far on the best therapeutic approach. The main objects of this study were to report the long-term results and to evaluate the importance of some possible prognostic factors in a large series of patients. NHL was considered primary gastric if the main symptoms at presentation were those of gastric disease. METHODS AND STUDY DESIGN: We analyzed 252 consecutive patients treated between 1980 and 1993 in five hospitals in north-east Italy. According to the Working Formulation, 98 patients had low grade lymphoma, 59 intermediate grade (D to F), 81 G or high grade and 14 were not classified. The patients were divided into two groups: one including patients with limited disease (localized to the stomach or perigastric lymph nodes: 165 patients) and one including those with advanced disease (87 patients). The treatment consisted of surgery, chemotherapy, radiotherapy or combinations of these. Sixteen patients received only supportive therapy. RESULTS: The five-year overall survival was 65.4%: 80.3% for patients with limited disease and 36.7% for those with advanced disease (P < 0.0001). Among the limited disease patients the five-year survival was 84.4% for those treated with gastrectomy alone and 88.7% for those who received also adjuvant chemotherapy (P = 0.11). However, while chemotherapy did not improve survival in low grade NHL, it seemed to produce a better survival in the intermediate and high grade groups (P = 0.06). Twelve patients were treated with primary chemotherapy and the five-year survival was 71.2%. In multivariate regression analysis the most important variable for overall survival was surgery for the whole group of 252 patients (P < 0.0001), while it was age for the group with limited disease (P = 0.0008). CONCLUSIONS: Surgery alone can be curative for most patients with gastric lymphoma limited to the stomach or to the perigastric lymph nodes; surgery followed by chemotherapy seems to produce better results than surgery alone in intermediate and high grade lymphomas. Also a non-surgical approach with first-line chemotherapy is associated with a high rate of complete remissions and five-year survival. In advanced disease the five-year survival is similar to that of nodal NHL.

Adolescent↗