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Biomedical subjects

C D Miller

Publications and source records attributed to C D Miller.

At least 73 records · Page 4Linked to original sources
PubMed1982

Fetal rat development as influenced by maternal lead exposure.

The teratogenic and neurologic effects of lead acetate on fetal rat development were investigated. Thirty-six female hooded rats were assigned to 4 treatment groups (0, 50, 75, and 100 mg/kg) and given daily oral doses of lead acetate. Animals were treated for 3 weeks prior to breeding and continuing throughout gestation. Rats were euthanized at day 20 of gestation. Blood sampling indicated that maternal blood lead concentrations in treated dams were maintained during gestation. Lead exposed groups had significant (at least p less than .01) maternal kidney and liver as well as fetal kidney lead content. Conceptus weight was significantly reduced in treatment groups. It was concluded that although significant amounts of lead crossed the placenta, as exemplified by fetal kidney values, no teratogenic response or reduction in fetal brain DNA content was produced in the rat.

Animals↗
PubMed1980

A technique developed for isolated canine papillary muscle function in various plasmas; and utility ascertained with plasma containing myocardial depressant factors.

Isolated papillary muscles from young dogs were studied to determine the feasibility of utilizing plasma, without added foam depressants, as a test solution to support muscle function. Diluted canine plasma, undiluted canine plasma, plasma with adjusted protein content, monkey plasma, and plasma containing myocardial depressant factors (MDF) were evaluated. For each test, each papillary muscle served as its own control, with either Krebs-Henseleit solution or a control plasma to establish a basis for evaluation of the test plasma. A technique was implemented to oxygenate the muscle, in plasma, without foam production and to monitor pH continuously. These studies indicated that plasma (diluted, undiluted, or with protein adjustments to accommodate experimental procedural requirements) was a satisfactory test solution. Furthermore, plasma from species unrelated to the dog could be utilized. In utilizing plasma, it was essential to establish or adjust the pH and osmolarity for normal muscle function. These studies with plasma containing MDF indicated that MDF depressed canine papillary muscle function. The Vmax values and Po values were decreased and latency was increased. The effects of MDF in canine papillary muscle preparations were completely reversible in short-term studies (up 10 10 hours' duration).

Animals↗
PubMed1975

Toxic effects of a new boron containing heterocycle: 4,4,8,8-tetraethyl-3,3a,4,8-tetrahydro 3a,4a,4-diazabora-S-indacene.

LD50 values in mice for 4,4,8,8-tetraethyl-3,3a,4,8-tetrahydro-3a,4a,4-diazabora-S-indacene (Myborin) were determined by the ip, po, and sc routes. The LD50 value for ip was 69.5 mg/kg found by the method of Litchfield and Wilcoxon, with upper and lower confidence limits of 77.8 and 62.1 mg/kg. Oral and sc LD50's were approximated after the method of Deichmann and LeBlanc and were found to be 180 mg/kg (po) and 420 mg/kg (sc). Each of these values has a confidence range of +/- 30%. Myborin induced convulsions, hyperreflexia, and death accompanied by tetany when given by either the ip or oral routes. Moreover, Myborin induced porphyria in animals surviving for 24 hr after these routes of administration and in virtually all animals dosed sc. Death by the sc route is probably a result of acute porphyria. Hepatomegaly and skin photosensitivity were demonstrated to be profound. Boron levels in the livers of mice were determined colorimetrically 24 hr after ip injections of Myborin and in untreated control mice. The quantity of boron found in the experimental group was 15.46 mug/g wet liver as compared to 8.11 mug/g wet liver for controls (P less than 0.01). The difference corresponds to 23% of the injected quantity of boron.

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