[Amylase determination in urine].
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Biomedical subjects
Publications and source records attributed to C D Koch.
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Results obtained with the commercial test-set "GOD-UV" (Dr. Haury, München) are reported. This method permits the determination of serum-glucose within 3 minutes. The procedure is satisfactory in normal and hyperglycaemic patients. In the hypoglycaemic state, however, there is a danger of misinterpretation. The glucose oxidase-UV is compared with the hexokinase reaction.
A new determination of esterases and arylesterases (E. C. 3.1.1.2.) with 4-nitrophenylacetate is described. The results are compared with those of the method described by Pilz using phenylacetate. 4-nitrophenylacetate instead of phenylacetate allows a kinetic reaction. The precision of the determination is good, the method easy to handle. Normal values: 1100--2600 U/1 (total esterases), 700--2100 U/1 (arylesterases) at +25 degrees C.
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