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Biomedical subjects

C D Hufford

Publications and source records attributed to C D Hufford.

61 records · Page 4Linked to original sources
PubMedDate not supplied

Microbial transformation studies on arteannuin B.

The microbial transformation of the sesquiterpene lactone arreannuin B [3] using Aspergillus flavipes produced dihydroarteannuin B [4] as the main transformation product. Preparative-scale fermentation of 3 with Beauveria bassiana, on the other hand, has resulted in the production of two metabolites, 3 beta-hydroxyarteannuin B [5] and 13-hydroxy-11-epi-dihydroarteannuin B [6]. The structure of these metabolites, all of which are new compounds, was established using chemical and spectroscopic techniques. The isomeric dihydrocompound, 11-epi-dihydroarteannuin B [7] and an isomer of arteannuin B [8] were also prepared chemically. All compounds were subjected to 2D-nmr experiments and full 1H- and 13C-nmr assignments were made.

Artemisinins↗
PubMedDate not supplied

Antifungal activity of Trillium grandiflorum constituents.

EtOH extracts of the rhizomes and aboveground portion of Trillium grandiflorum showed significant antifungal activity. Bioassay directed fractionation has led to the identification of the active components as the saponin glycosides 1 and 3.

Animals↗
PubMedDate not supplied

Microbial metabolism studies of the antimalarial sesquiterpene artemisinin.

Microbial metabolism of the sesquiterpene lactone antimalarial drug artemisinin [1] was studied. Screening studies have shown a number of microorganisms capable of metabolizing artemisinin [1]. Scale-up fermentation with Nocardia corallina (ATCC 19070) and Penicillium chrysogenum (ATCC 9480) have resulted in the production of two major microbial metabolites that have been characterized with the use of 2D-nmr techniques. These metabolites have been identified as deoxyartemisinin [2] and 3 alpha-hydroxydeoxyartemisinin [3].

Antimalarials↗
PubMedDate not supplied

Preparation, characterization, and antiviral activity of microbial metabolites of stemodin.

Screening studies for microbial transformation products of stemodin [2] have identified a number of microbial metabolites. Scale-up fermentation with Rhizopus arrhizus ATCC 11145 and Streptomyces sp. NRRL 5691 have resulted in the production of five metabolites that have been characterized with the use of 2D nmr and X-ray techniques. These metabolites have been identified as 18-hydroxystemodin [6], 16,18-dihydroxystemodin [7], 8 beta-hydroxystemodin [8], 8 beta, 18-dihydroxystemodin [9], and 7 beta, 8 beta-dihydroxystemodin [10]. The antiviral activity and cytotoxicity of the isolated metabolites have been evaluated.

Animals↗
PubMedDate not supplied

Antimicrobial activities of constituents of Uvaria chamae.

The antimicrobial activities of a number of cytotoxic C-benzylated flavonoids from Uvaria chamae have been determined. The minimum inhibitory concentration values of these flavonoids and certain of their derivatives against Straphylococcus aureus, Bacillus subtilis, and Mycobacterium smegmatis compare favorably with those of streptomycin sulfate.

Antibiotics, Antineoplastic↗
PubMedDate not supplied

Two metabolites from Aspergillus flavipes.

Two novel fungal metabolites, N-benzoyl-L-phenylalaninol (1a) and asperphenamate (2) were isolated from the culture filtrate and mycelium of Aspergillus flavipes ATCC 11013. N-benzoyl-L-phenylalaninol was identified by direct comparison with an authentic sample. The structure of asperphenamate is proposed as (S)-N-benzoyl-phenylalanine-(S)-2-benzamido-3-phenyl propyl ester, based on chemical and spectroscopic evidence.

Aspergillus↗