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Biomedical subjects

C D Baker

Publications and source records attributed to C D Baker.

8 recordsLinked to original sources
PubMed1991

Urokinase binding and receptor identification in cultured endothelial cells.

Recombinant human single-chain urokinase (rscu-PA), two-chain urokinase (tcu-PA), and diisopropyl-fluorophosphate-treated tcu-PA (DFP-tcu-PA) bound to cultured human and porcine endothelial cells in a rapid, saturable, dose-dependent and reversible manner. Analysis of specific binding results in cultured human umbilical vein endothelial cells (HUVECs) gave the following estimated values for Kd and Bmax: 0.57 +/- 0.08 nM (mean +/- S.E.) and 188,000 +/- 18,000 sites/cell for 125I-labeled rscu-PA; 0.54 +/- 0.10 nM and 132,000 +/- 23,900 sites/cells for 125I-labeled tcu-PA; 0.89 +/- 0.14 nM and 143,000 +/- 30,300 sites/cell for 125I-labeled DFP-tcu-PA, respectively. Values for Kd were similar for primary and subcultured (six passages) HUVECs, but Bmax values were lower in subcultured HUVECs. Similar Kd values were found in cultured porcine endothelial cells; however, Bmax values varied depending on the endothelial cell type. All 125I-labeled urokinase forms yielded similar cross-linked approximately 110-kDa ligand-receptor complexes with cultured HUVECs, and 125I-labeled DFP-tcu-PA bound to a single major approximately 55-kDa protein in whole-cell lysates (ligand blotting/autoradiography), suggesting the presence of a single major approximately 55-kDa urokinase receptor in cultured HUVECs. The approximately 55-kDa urokinase receptor, isolated from several separate batches of cultured HUVECs (3-5 micrograms of protein, approximately 1 x 10(9) cells), by ligand affinity chromatography, exhibited the following properties: retained biologic activity as evidenced by its ability to bind 125I-labeled rscu-PA by ligand blotting/autoradiography and formation of a cross-linked 125I-labeled approximately 110-kDa rscu-PA-receptor complex; single-chain approximately 55-kDa protein, following reduction; complete conversion to and formation of a single major deglycosylated approximately 35-kDa protein, following treatment with N-glycanase.

Animals↗
PubMed1991

Long-term postoperative dysphagia in oral/pharyngeal surgery patients: subjects' perceptions vs. videofluoroscopic observations.

Dysphagia commonly results from surgical resection of various structures within the oral, pharyngeal, and esophageal areas. The type and severity of swallowing dysfunction are based largely on the number and quantity of structures removed as well as the reconstructive procedure. Short term-recovery has been addressed in many studies. However, follow-up studies of long-term functional results and continuing swallowing problems following oral/pharyngeal surgery are unavailable. In this study, perception of swallowing dysfunction was compared with actual videofluoroscopic findings in subjects at least 1 year after oral/pharyngeal surgery. The comparative results of a questionnaire developed to evaluate patients' perception of continuing swallowing difficulty and an analysis of actual videofluoroscopic tapes indicated that the degree or type of dysphagia could not be determined from patients' subjective descriptions of the swallowing problem.

Adult↗
PubMed1987

Testing the validity of categories in a theoretical perspective on chronic illness.

The purpose of this study was to analyze differences in future time perspective, loneliness and perceived maternal expressiveness between adolescents who were chronically ill with cystic fibrosis and adolescents who were reportedly healthy. The sample consisted of 30 adolescents with cystic fibrosis who were matched on selected variables with 30 healthy adolescents. They responded to the Heimberg Future Time Perspective Inventory, the Revised UCLA Loneliness Scale, and the Perceived Maternal Expressiveness Scale. The three hypotheses were tested using the t-test. Since none of the hypotheses was supported, the validity of the categories was questioned. Theoretical and practical implications are addressed.

Adolescent↗
PubMed1979

Effect of general practitioners' advice against smoking.

During four weeks all 2138 cigarette smokers attending the surgeries of 28 general practitioners (GPs) in five group practices in London were allocated to one of four groups: group 1 comprised non-intervention controls; group 2 comprised questionnaire-only controls; group 3 were advised by their GP to stop smoking; and group 4 were advised to stop smoking, given a leaflet to help them, and warned that they would be followed-up. Adequate data for follow-up were obtained from 1884 patients (88%) at one month and 1567 (73%) at one year. Changes in motivation and intention to stop smoking were evident immediately after advice was given. Of the people who stopped smoking, most did so because of the advice. This was achieved by motivating more people to try to stop smoking rather than increasing the success rate among those who did try. The effect was strongest during the first month but still evident over the next three months and was enhanced by the leaflet and warning about follow-up. An additional effect over the longer term was a lower relapse rate among those who stopped, but this was not enhanced by the leaflet and warning about follow-up. The proportions who stopped smoking during the first month and were still not smoking one year later were 0.3%, 1.6%, 3.3%, and 5.1% in the four groups respectively (P <0.001).The results suggest that any GP who adopts this simple routine could expect about 25 long-term successes yearly. If all GPs in the UK participated the yield would exceed half a million ex-smokers a year. This target could not be matched by increasing the present 50 or so special withdrawal clinics to 10 000.

Adolescent↗
PubMed1976

Non-attenders in general practice.

Non-attenders for five years or longer (77 men and 27 women; i.e. 1.9 per cent of the practice) in a general practice of 5,750 patients were identified and paired with controls in the same practice. A survey by questionnaire was carried out.The conclusions were:(1) A higher proportion of non-attenders are self-employed,(2) A lower proportion of them had retired than their attending counterparts,(3) They are spread over the whole age-range, but with a higher concentration in the ages 35 and over,(4) They are evenly spread over the social classes,(5) They seldom seek other sources of medical advice or treatment,(6) They are significantly slimmer than their fellows,(7) Their non-attendance does not, on the whole, cloak serious but remediable illness.

Adolescent↗
PubMedDate not supplied

The feasibility of bench tests for disposable ECG electrode adhesiveness.

The feasibility of recommending one standard bench test that adequately qualifies electrode adhesives for clinical use was examined by UBTL. Small strips, cut from whole, commercially available disposable ECG electrodes, were attached to three types of surfaces, and samples of each type of electrode were peel tested at 15 minutes, 8 hours, and 48 hours after attachment. A program was developed to average, display, and calculate peel strength parameters. Analysis of the data showed that (a) the peel strength of electrodes varied by more than a factor of 10 when measured on human skin, and by more than a factor of 3 when measured on bench test surfaces, and (b) the peel strength of the electrode adhesive strips did not correlate with intact electrode loss on ambulatory subjects. Therefore, UBTL could not recommend one bench test that would measure adhesion performance for all electrode types appropriate for inclusion in an AAMI ECG electrode standard.

Adhesiveness↗