Biomedical subjects

C Czerkinsky

Publications and source records attributed to C Czerkinsky.

96 records · Page 6Linked to original sources

PubMed1985

Simultaneous induction of rheumatoid factor- and antigen-specific antibody-secreting cells during the secondary immune response in man.

The production of anti-tetanus toxoid antibody and rheumatoid factor (RF) by individual peripheral blood mononuclear cells (PBMC) was studied at various times after booster immunization of healthy volunteers with tetanus toxoid (TT). TT-specific antibody-secreting cells and RF-secreting cells were assayed by the enzyme-linked immunospot (ELISPOT) assay. Cells obtained 5-9 days after immunization were found to spontaneously secrete TT-specific antibodies. This response was specific for the immunogen, was abrogated by cycloheximide and accounted for most of the immunoglobulins produced. TT-specific antibody-secreting cells were found concomitantly with cells secreting RF, mainly of the IgM isotype. Elevated levels of circulating immune complexes (CIC) containing IgG and C3 were observed shortly before the appearance of both TT-specific antibody-secreting cells and IgM RF-secreting cells. Thus, after booster immunization with TT, two major subpopulations of immunoglobulin-secreting cells appear concomitantly in the peripheral circulation: a population of cells secreting TT-specific antibody and a population of cells secreting IgM RF. It is suggested that RF production in man represents a physiological event which might be triggered by IgG-containing immune complexes formed during a secondary immune response.

Adult↗

PubMed1984

Solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of IgG rheumatoid factor-secreting cells.

Although IgG rheumatoid factor may play an essential role in the pathogenesis of rheumatoid arthritis, there is no precise method for its specific detection at the cellular level. A modification of the recently developed enzyme-linked immunospot assay has been devised for enumeration of cells secreting IgG rheumatoid factor (IgG RF) and simultaneous quantitation of the IgG RF secreted. Specific, sensitive and simple, this new assay should provide a valuable tool for study of isotype-specific RF secretion by single cells.

Animals↗

PubMed1984

Detection of single antibody-secreting cells generated after in vitro antigen-induced stimulation of human peripheral blood lymphocytes.

Culture and assay procedures are described for the generation and the subsequent detection of single antigen-specific antibody-secreting cells after in vitro stimulation of human peripheral blood lymphocytes with tetanus toxoid. Simple, specific and sensitive, this new assay-culture system is well suited for the analysis of specific antibody production in man.

Adult↗

PubMed1984

Detection of IgG rheumatoid factor secreting cells in autoimmune MRL/1 mice: a kinetic study.

The kinetics of appearance of cells secreting IgG rheumatoid factor (IgG RF) has been studied in MRL/l mice by means of the ELISPOT assay, a new immunoenzyme procedure. Mice of the MRL/l strain spontaneously develop an autoimmune disease associated with arthritic manifestations. IgG RF secreting splenocytes were first detected at the clinical onset of the disease at the age of 3 months. Peak frequencies of IgG RF secreting cells amounting to almost 10% of the total number of IgG secreting cells were observed at later stages. The appearance of IgG RF secreting cells was preceded by at least 1 month by elevated levels of circulating immune complexes and abnormally high numbers of IgG secreting cells. The frequencies of IgG RF secreting cells and of IgG secreting splenocytes did not follow each other at least not during the first 3 months preceding the onset of clinical disease. The data presented suggest that IgG RF production in MRL/l mice is not triggered as part of an early stage of polyclonal activation but could be induced by IgG containing immune complexes.

Aging↗

PubMed1984

Natural antibodies in man to Streptococcus mutans: specificity and quantification.

Antibodies to whole cells of Streptococcus mutans were examined in 108 subjects by a solid-phase radioimmunoassay and quantified by reference to isotype-specific affinity-purified antibodies. Serum antibodies of each isotype were present in all subjects examined. The mean concentration of serum antibodies to S. mutans was calculated as about 84 micrograms/ml of IgG (range 33-140 micrograms/ml), 26 micrograms/ml of IgA (range 12-43 micrograms/ml) and 9 micrograms/ml of IgM (range 4-15 micrograms/ml). The mean antibody values accounted for about 0.7, 1 and 0.8% of the total IgG, IgA, and IgM, respectively. Overall the antibody binding to whole cells of S. mutans accounted for about 0.8% of the total immunoglobulin. Inhibition experiments using a variety of purified cell wall antigens revealed that the binding of antibodies to whole cells could be inhibited by about 30% with a purified protein antigen (SA I/II) and with glucosyltransferase (GTF), by 25% with c polysaccharide and by 16% with lipoteichoic acid. The protein antigens GTF and SA I/II appear to be major immunogenic cell wall antigens, but natural antibodies in man that bind to S. mutans whole cells have been induced by several antigens, some of which are specific to S. mutans and some of which are shared with other Gram-positive bacteria.

Adolescent↗

PubMed1983

The detection and specificity of class specific antibodies to whole bacterial cells using a solid phase radioimmunoassay.

A solid phase radioimmunoassay has been developed which can be used for the detection of isotype specific antibodies to whole bacteria and other particulate antigens, and is applicable to a variety of species. Bacteria are bound to the solid phase by the use either of antibodies, or of methyl glyoxal. Both methods result in a sensitive and reproducible assay, and bacteria do not appear to desorb from the solid phase. The specificity of antibodies to whole bacteria was examined by absorption of antisera with various species of bacteria and retesting, or by determining the binding of antisera to various bacteria bound to the solid phase. Both methods revealed specificity for the bacteria examined. Inhibition studies showed that antibodies to Streptococcus mutans whole cells could be inhibited by purified cell surface antigens glucosyltransferase and antigen I/II, but only minimally by lipoteichoic acid, c polysaccharide or dextran. In murine antisera antibodies of the IgG, IgM, and IgA classes could be detected at amounts of less than 1 ng/ml.

Actinomyces↗