Biomedical subjects
Publications and source records attributed to C Cochet.
Incorporation of ouabain (G-strophantin) in the culture medium 24 h after that of Sandai virus, at a final concentration of 2.10(-6)M/1, increases considerably the yield of man/mouse or primate/mouse cellular hybridization.
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The karyotype of Cercopithecus aethiops was analysed by RHG, GTG, and C banding, and a band nomenclature is proposed.
Cytogenetic and biochemical analysis of 10 independant Chimpanzee-Mouse cell hybrids and of 18 subclones of one of these showed that PPH, PGM1 and Pep-C are localized on the Chimpanzee chromosome homologous to the human chromosome No. 1.
Ten independant cellular hybrids were obtained from Chimpanzee (Pan troglodytes) fibroblasts and the murine cell line C11D. The comparison of electrophoretic and cytogenetic studies showed that 9 markers with known localizations in Man could also be localized on the homologous chromosomes of the Chimpanzee: pyrophosphate hydratase (PPH), phosphoglucomutase-1 (PGM1), and peptidase-C (Pep-C) on the No. 1; malate dehydrogenase MDH(NAD) on the No. 2; lactico dehydrogenase-A (LDH-A) on the No. 11; lactico dehydrogenase-B (LDH-B) on the No. 12; thymidine kinase (TK) on the No. 17; superoxide dismutase-1 (SOD-1) on the No. 21; glucose-6-phosphate dehydrogenase (G6PD) on the X. The localization of mannose phosphate isomerase (MPI) on the No. 7 could be excluded. One discrepancy between Chimpanzee and Man was noted: the localization of superoxide dismutase-2 (SOD-2) on the 6 is excluded.
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The various protein kinase activities characterized in adrenal cortex from different mammalian species do not differ in their molecular and catalytic properties from similar types of enzymes described in other tissues. The cyclic AMP-dependent system has been the most thoroughly investigated in adrenocortical tissue and is generally accepted as an obligatory intermediate in the intracellular response to ACTH action. In addition, adrenocortical cells contain two types of messenger independent protein kinases, one of which being selectively activated by polyamines in vitro and possibly related to the control of adrenocortical growth processes. The definition of intracellular phosphorylatable targets related to specific adrenocortical functions might have been obscured by the pleiotypic effects displayed by ACTH, the major effector of these cell activities. Cholesterol esterase appears one of these targets and 11 beta-hydroxylase cytochrome P-450 has been found activated in vitro by a cAMP dependent process. Future research should provide a better understanding of the role of protein phosphorylation-dephosphorylation processes as a regulatory device in adrenocortical cell functions. This includes possible complex modulation by multisite phosphorylations of a given target protein, involving several different protein kinase systems under the dependence of separate intracellular effectors.