Biomedical subjects

C Chen

Publications and source records attributed to C Chen.

At least 397 records · Page 22Linked to original sources

PubMed2000

[Prenatal diagnosis of fetal congestive heart failure by color flow imaging and pulse Doppler ultrasound].

OBJECTIVE: To investigate prenatal diagnosis of fetal congestive heart failure (CHF) by Color Doppler Flow Imaging (CDFI) and pulse Doppler Ultrasound (PU). METHODS: 3,841 pregnant women were detected by ultrasound. Maternal-placental-fetal circulation surveillance by high resolution Doppler ultrasound was performed in 302 high-risk pregnancies. The diagnosis of CHF is dependent upon (1) The cardiac area/chest area ratio > 0.33; (2) An abnormal umbilical vein pulsation; and (3) Tricuspid regurgitation. RESULTS: There were six deaths that all had abnormal umbilical vein pulsation and five cases who had C/T area ratio > 0.33. CHF accounted 0.17% in all the pregnancies and 2% in high-risk pregnancies. CONCLUSIONS: The results suggest that blood velocity recording abnormal pulsed sign in the umbilical veins and C/T area ratio of the fetus can give valuable clinical information with regard to the presence of fetal CHF. Fetus with CHF almost always had poor outcome. CDFI and PU may have implications for fetal diagnostic work-up and prognosis.

Adult↗

PubMed2000

[Posttransplantation diabetes mellitus].

OBJECTIVE: To study the clinical rules of posttransplantation diabetes mellitus (PTDM). METHODS: 21 patients treated from Dec. 1992 to Sept. 1998 were analysed. RESULTS: The morbidity of PTDM was 16.3% of total patients in the corresponding period. Between the groups of PTDM and NON-PTDM, there was statistical significance in age, kidney function and family diabetic history. It was not related to CSA bottom concentration and acute rejection. In this group 2 cases were complicated by hyperosmolar nonketotic diabetic comas (9.5%) and 11 various infections (52.4%). CONCLUSIONS: PTDM as a secondary diabetes due to antirejection drugs and allograft kidney function may cause serious consequences if it is treated improperly.

Adult↗

PubMed2000

[The ultrastructure of human and mouse cataractous lens epithelial cell apoptosis].

OBJECTIVE: To observe the relation between human senile cataract, mouse congenital cataract and lens epithelial cell apoptosis. METHODS: Transmission electron microscope was used to observe the apoptotic morphology of the lens epithelial cells in 4 cases with senile cataract and in 2 mice with congenital cataract. The comparisons were made between the cataractous and normal transparent lens epithelial cells in human being and mice. RESULTS: The apoptotic cells were found in senile cataract and mouse congenital cataract lens epithelial cells, while in all control lens epithelial cells they were few. CONCLUSION: Lens epithelial cells apoptosis is related to senile cataract and mouse congenital cataract formation.

Animals↗

PubMed2000

[The histogenesis, clinical features and histopathological analysis on 52 cases of ciliary body neoplasms].

OBJECTIVE: To analyze the histogenesis, classification, incidence, clinical feature, the key point of differential diagnosis and histopathologic characteristics of ciliary body neoplasms to provide the basis for clinical diagnosis and treatment. METHOD: 52 specimens of ciliary body neoplasms on file were analyzed and studied for the characteristics of clinical presentation and histopathology. RESULTS: Of the 52 specimens, 38 were located in the ciliary body (73.1%), and 14 lesions involved the iris (26.9%). According to the results of histogenetic classification, the tumors of ciliary sensory epithelium were ranked the first place (20 cases, 38.5%) while malignant melanoma, the second one (14 cases, 26.9%) followed by melanocytoma (9 cases, 17.3%) and granulomatous lesions (5 cases, 9.6%). CONCLUSION: The clinical features and histopathologic characteristics of ciliary body neoplasms are systemically and deeply recognized, that may help clinical ophthalmologists to make correct diagnosis and proper treatment.

Adolescent↗

PubMed2000

[An experimental research of taurine on H2O2-induced bovine lens epithelial cell apoptosis].

OBJECTIVE: To study the inhibitory role of taurine on H(2)O(2)-induced bovine lens epithelial cell apoptosis. METHODS: By terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling (TUNEL) detection and bovine lens organ culture, we examined the affect of taurine on the number of H(2)O(2)-induced bovine lens epithelial cells with apoptosis, the related comparisons and statistic analyses were carried out. RESULTS: (1) Lens epithelial cell apoptosis began before lens opaqueness (lens became opaque after 6 hours of incubation, while the lens epithelial cell apoptosis was detected after 3 hours of incubation); (2) Taurine could apparently inhibit H(2)O(2)-induced bovine lens epithelial cell apoptosis. CONCLUSION: Taurine has an inhibitory role on H(2)O(2)-induced bovine lens epithelial cell apoptosis and can delay and ameliorate the occurrence and development of cataract.

Animals↗

PubMed2000

[A cross-sectional study on the effects of microcystin in drinking water to the health of human beings].

OBJECTIVE: To understand the effects of microcystin (MC) in drinking water on health of human beings. METHODS: In Taixing, a cross-sectional study was conducted in two middle schools with different types of drinking water, from which 111 and 92 students were randomly selected and investigated. Blood samples from all individuals were collected and tested for serum HBsAg, HBeAg, anti-HBe, anti-HBs, anti-HBc with enzyme linked immunoabsorbent assay and for serum alanine aminotransferase (ALT), gamma-glutamyltransferase (gamma-GT) and alkaline phosphatase (ALP) contents by colorimetry. These levels of enzymes in sera were compared after the students were stratified according to the status of HBV infection. RESULT: With the exclusion of the effect of HBV infection, three enzymes levels in sera between two groups of students showed significant differences (P < 0.01), much higher in exposure group than those in control group. CONCLUSION: MC was possibly harmful to human beings in drinking water and induced the contents of some enzymes from the liver of human beings to increase in sera.

Adolescent↗

PubMed2000

[Study on beta-adrenoceptors mechanism of cadmium immunotoxicity].

OBJECTIVE: To study if the beta-adrenoceptors mechanism plays important role in the immunotoxicity of cadmium. METHODS: The beta-adrenoceptor density, cellular cyclic adenosine 3',5' monophosphate (cAMP) levels, and DNA damage of rat spleen lymphocytes were studied. Beta-antagonist propranolol was used to block the beta-adrenoceptor. RESULTS: In the single Cd administration group: beta-adrenoceptor density of spleen lymphocytes in the middle and high Cd concentration groups was higher than that in the non-Cd treated control group (P < 0.05), the levels of cAMP increased with the increase of Cd doses, and the result of the high Cd group was higher than that of the control group (P < 0.05). The proliferation function of T lymphocytes was lower in each Cd group than that in the control group (P < 0.05). Single cell gel electrophoresis (SCGE) detected that DNA damage of spleen lymphocytes in each Cd group were more severe than the control (P < 0.05). Pretreated with Pro group: After blocked with propranolol, beta-adrenoceptor density increased in the Pro control group and the density in all Cd treated groups was higher than the control group (P < 0.05), but all Cd treated groups showed no statistical significance than the Pro control group (P > 0.05). The cAMP levels, MTT and the subsets of T lymphocytes showed no statistical significance among all the groups (P > 0.05). The results of SCGE showed that DNA damage was most severe only in the high Cd group than other groups (P < 0.05). CONCLUSION: The results suggest that the nerve-internal secretion system plays an important role in the immunotoxicity of cadmium, and the beta-adrenoceptor mechanism is important in the immunotoxic mechanism of cadmium.

Adrenergic beta-Antagonists↗

PubMed2000

[General report on food and nutrition surveillance in 1998 and policy recommendation].

Based on the data of collection, nutrition status of children, food consumption and nutrition of urban and rural residents are analyzed, and related policy recommendations are presented. The improvement of the nutritional status of children in China is the consequence of socioeconomic development. The strategy for child nutrition improvement should focus on rural area and complementary feeding should be a key approach as important as breastfeeding to be stressed on. Immediate set priority on approaches promoting consumption production and processing of soybean. Nutrition problem of the poor should and could be resolved to consolidate the achievement of the National Poverty Alleviation Program.

Adult↗

PubMed2000

[Current situation and trend of child growth in China].

Data of Food and Nutrition Surveillance in 1990, 1995 and 1998 was analyzed; it indicated the evident improvement of weight and height of children under 5 in the past eight years. The prevalence of underweight were reduced from 8.0% in 1990 to 2.7% in 1998 in the urban, and in the rural, the prevalence were 22.0% and 12.6% respectively. The reduction of prevalence of stunting in the urban decreased from 9.4% to 4.1%, and in the rural from 41.4% to 22.0% in the same period. During 1990-1995, weight of children under 5 went up evidently, but linear growth improvement was very slow and did not match the weigh gain. Height of children increased in a higher speed during 1995-1998. Height and weigh of age groups indicated that 6-18 month was the critical period for prevention of malnutrition of children under 5. The paper further analyzed the favorable changes of the attributable factors of malnutrition, such as diarrhea, safe drinking water, breastfeeding, education level of mothers, household diet and income during this period. The conclusion is the remarkable nutrition improvement of children under 5 in 1990s is the consequence of the rapid socio-economic development of China.

Adult↗

PubMed2000

[Relationship between growth of young children and complementary feeding].

Description analysis, correlation regression analysis and logistic regression analysis were applied to evaluate the impact of complementary food on the growth of children from the aged 4 to 24 months, results on the effect of the complementary feeding were presented. Among children under 2 years in poor rural areas, 42% of them got complementary food containing animal food, 30.5% got milk, 57.8% got starchy foods, and 48.6% got vegetables and fruits. The coverage of complementary food containing animal food, vegetables/fruits was only half of that in the urban areas. Analysis revealed significantly positive correlation of HAZ, WAZ of all age group of children under 2 years with the appearance of animal foods, vegetable and fruit in the complementary food, but there was no significantly correlation with starchy foods. According to the regression coefficient, it was estimated that the prevalence of stunting aged 18-24 months could be reduced 2.6 percentage points if 10% increase of coverage of animal food containing complementary food in the CFNSS site was achieved. Logistic regression analysis showed the odds ratio (OR) of non-animal food complementary food for stunting was 2.21 for children aged 4-6 months, 1.73 for 6-12 months, 2.81 for 12-18 months, 2.58 for 18-24 months, OR for underweight was 2.87 for 12-18 months, and 2.08 for 18-24 months.

Child Development↗

PubMed2000

[Status and economic losses of respiratory infection and diarrhea of children under 6 years].

Based on the data collected from the CFNSS in 1998, the status and trends of respiratory infection and diarrhea of children and the economic losses caused by the two diseases were analyzed. The prevalence of two-week respiratory infection of urban children under 6 was 23.75% and 21.97% in the general rural, and 26.03% in poor rural. The peak of respiratory infection of rural children was at 6-18 months, but in the urban, that was at age of 3-4 years. Before 2 year of age, the respiratory infection was higher in the rural, and after that age it was higher in the urban. Since 1990, the prevalence of respiratory infection went up and increased 9.5 percentage points over 8 years in the urban, the percentage point of increase was 6.9 in the rural during the same period. In 1998, the prevalence of diarrhea of rural children under 6 was 7.0%, higher than in the urban, which was 4.3%. The prevalence reduced from 9.4% to 4.3% during 1990-1998 in the urban, and in the rural from 13.0% to 7.0%. Weight and height of children with diarrhea incidence were significantly lower than children without diarrhea. Calculation based on the cost for the episodes of the two diseases and the disease prevalence was made and estimate of the economic losses was 11,793 yuan (RMB) for urban, 5458 yuan (RMB) for rural areas.

Child↗

PubMed2000

[Food consumption and dietary pattern in China during 1990-1998].

Based on data of food consumption collected in the household survey of State Statistics Bureau in 1990 and 1995, consumption of soybeans and its production decreased in all household. Urban population over-consumed animal food, intake of cereals reduced and energy share of cereals was dropped to less than 60%. Rural population consumed mainly cereals, animal food, fruit and oil consumption was lower. 1998 data showed that the animal food consumption increased along with income increase, but vegetable oil consumption kept stable. Dietary pattern of urban household were still relatively rational, but animal food consumption still needed to be increase in the rural households, and more attention should be paid to the poor households. Enhancement of nutrition education and promotion of soybean consumption and production for nutrition improvement for both urban and rural population was recommended to make urban diet to meet the goals of a balanced diet and the rural diet to be nutritionally adequate.

China↗

PubMed2000

[Assessment of dietary pattern in China with desirable dietary pattern].

Applying desirable dietary pattern (DDP) to analyze the quality of the diet in urban and rural household in 1995 and 1998 was presented. The results showed that: urban people should adjust the structure of their diet, to level down the increasing trend of animal food, as a replacement, more soybean consumption should be encouraged. Rural people should increase moderate amount of animal food and oils, and increase of vegetable/fruits and overall non-staple foods could keep the diet quantitatively and qualitatively improved and balanced. Promotion of soybean consumption in the rural households was the most feasible and effective way of improving nutritional quality of the diet in the rural in the near future.

China↗

PubMed2000

[Nutrition and economic development of poor areas].

The relationship between nutrition and economic development is discussed, it illustrates that nutrition is not only the consequence of economic development, but also as one of the motive power, playing important role in economic development. The authors reviewed the scientific insight of the impact of malnutrition on disease prevalence, mental development, adult physical capability and productivity. Data on nutrition status of population in the poor areas and the analysis on the risk of chronic disease poverty population facing to are presented. In order to protect the health of the poor, and to guarantee the physical efficiency and productivity of the current labor force in poor areas, and to provide a mentally and physically well developed manpower for the future economy, the authors appeal to the policy-makers to integrate nutrition component into poverty alleviation program. Several low cost and effective approaches for nutrition improvement are listed.

China↗

PubMed2000

[Studies on gene knocking out of 2-keto aldose reductases from Erwinia sp. SCB125].

Based on the reported gene sequences, the segments containing 2-keto aldose reductase (2-KRA and B) genes were amplified by PCR from the plasmids and Erwinia sp. SCB125 each for gene expression and gene knocking out. Then cloning them into expression vector pBL and successfully expressing them with high enzyme activity in E. coli DH5 alpha. After their enzyme activities were proved, the work on gene knock out followed. Introducing the knock-out vector which distribute unstably during the cell division to the host strains Erwinia sp. SCB125. Screening firstly by the positive marker, one resistance which resulted from the expression of the resistance gene inserting inside the reductase genes and the negative marker, another resistance which outside the reductase genes in the vector. The strains selected out will be tested by further study. This work was the bases of blocking the pathway metabolism and constructing a recombinant strain that can produce 2-KLG directly from D-glucose by one-step fermentation.

Aldehyde Reductase↗

PubMed2000

[Gene cloning and expression of prolyl endopeptidase from Aeromonas punctata].

Prolyl endopeptidase gene was cloned from Aeromonas punctata subsp. Punctata(ST-78-3-3) using activity screening method and the 3.3 kb DNA fragment containing PEP gene was sequenced. DNA sequence from 20-2092 bp was ORF region coding 690 amino acids of prolyl endopeptidase. It was a new PEP gene through gene search. The genetic engineered E. coli BL21/pGEM-PEP overexpressing recombinant Aeromonas punctata prolyl endopeptidase(apPEP) was constructed. Cultured in YH medium, the expressed apPEP was about 30% of total cellular protein, the activity was 112 times more than that of wild strain. Expressed apPEP was mainly soluble intracellular protein, about 5% of apPEP was secreted to medium. Non-reduced SDS-PAGE analysis showed it's monomer with molecular weight about 76 kD, which corresponded with prediction by gene sequence. Recombinant apPEP was purified after tube culture, the purity reached 90% and specific activity was 67 U/mg.

Aeromonas↗

PubMed2000

[The degradation performance of bioabsorbable acylchitin fiber reinforced PLA composite materials in vitro and in vivo].

The present authors have investigated the degradation performance of acylchitin fiber reinforced polylactide composite materials plates both in vitro and in vivo. The initial flexural strength and the initial flexural modulus of this plate are 114.72 MPa and 3980.05 MPa, respectively. The flexural strength of this plate decreases to 31.42 MPa after the plate has been submerged in injectio natrii lactatic ringeri tissue fluid for a period of 16 weeks at 37 degrees C. Both the in vitro degradation performance and the strength retention of this plate are better than those of the self-reinforced PGA/PLA and PGA, though the initial strength of the latter two being much higher than that of the former. The degradation products of the chitin/PLA composite materials can be absorbed by metabolic pathway.

Animals↗

PubMed2000

[Studies on the continuous culture and pathogenicity of Entamoeba gingivalis].

OBJECTIVE: To establish a method for continuous culture of Entamoeba gingivalis (E. g.) and study the pathogenicity of E. g.. METHODS: The culture conditions of E. g. were compared by observing its growth and reproduction. The pathogenicity of E. g. was studied by investigating its infection rate in humans and observing the relationship between E. g. infection and periodontal abscess in rats and the in vitro effect of some drugs. RESULTS: The optimal culture condition of E. g. was modified LES medium containing Locke's solution with 20% bovine serum, penicillin, streptomycin and rice starch. Reproduction of E. g. peaked at the fourth day of incubatoin, E. g. could live continuously for 120 to 168 h. The infection rate of E. g. was higher in patients with periondontal diseases than in healthy groups. The animal test showed that E. g. could cause periondontal abscess in rats. The levels of acid phosphatase and MDA were higher in patients with E. g.-positive periondontal disease than in healthy individuals. Metronidazole and SBF are effective against E. g.. CONCLUSION: E. g. could grow very well in modified LES medium, and cause periondontal abscess in patients with low immunity.

Adult↗